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Fish Physiology and Biochemistry

, Volume 38, Issue 2, pp 469–482 | Cite as

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

  • Anurag Dabas
  • N. S. Nagpure
  • Ravindra KumarEmail author
  • B. Kushwaha
  • Pavan Kumar
  • W. S. Lakra
Article

Abstract

The effects of different concentrations of cadmium chloride on the extent of lipid peroxidation (LPO) and alterations in the antioxidant enzyme activities were studied in liver, kidney and gill tissues of freshwater murrel, Channa punctatus. The fish specimens were exposed to 6.7, 13.4 and 20.1 mg l−1 sublethal concentrations of cadmium chloride and the oxidative stress was assessed after 24, 48, 72 and 96 h post-exposure. The biomarkers selected for the study were thiobarbituric acid reactive substances for assessing the extent of lipid peroxidation and antioxidant defense system such as reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) activities. In general, the cadmium exposure elevated the LPO in subject tissues of treated group and modulated the activities of GPx, GST, SOD, CAT, GR and level of GSH after given exposure as compared to the control. All enzymes activities, except CAT (in kidney and gills), and amount of LPO elevated significantly (P < 0.05) in treated group with respect to control in all tissues, while significant difference was not observed between the exposed concentrations and within exposure duration. The results indicated that increase in LPO level and the fluctuation in antioxidant defense system in fish could be due to cadmium-induced increase in the production of reactive oxygen species (ROS). The potential role of these parameters as biomarkers of heavy metal pollution in aquatic system is discussed.

Keywords

Antioxidant enzymes Cadmium Channa punctatus Lipid peroxidation Oxidative stress 

Notes

Acknowledgments

The authors are thankful to the Director, National Bureau of Fish Genetic Resources, Lucknow, India, for providing support materials and laboratory facility for conducting this study.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Anurag Dabas
    • 1
  • N. S. Nagpure
    • 1
  • Ravindra Kumar
    • 1
    Email author
  • B. Kushwaha
    • 1
  • Pavan Kumar
    • 1
  • W. S. Lakra
    • 2
  1. 1.National Bureau of Fish Genetic Resources, Indian Council of Agricultural ResearchLucknowIndia
  2. 2.Central Institute of Fisheries EducationMumbaiIndia

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