Fish Physiology and Biochemistry

, Volume 37, Issue 3, pp 567–572 | Cite as

Serum biochemical characteristics of Beluga, Huso huso (L.), in response to blood sampling after clove powder solution exposure

  • Seyyed Morteza Hoseini
  • Seyed Abbas Hosseini
  • Ali Jafar Nodeh


In order to investigate the effect of anesthesia on serum parameters, Beluga, Huso huso (L.) were blood-sampled immediately without anesthesia (control) or subjected to following anesthesia procedure: 40, 120, and 240 s exposure to 3,000, 700, and 500 mg l−1 clove solution, respectively. Blood samples were collected after these periods, when fish were immobile and reached stage 4 anesthesia. Results showed that cortisol and glucose levels were significantly high in 700 and 500 but not 3,000 mg l−1 group compared to control. Serum lactate levels were significantly high in 500 mg l−1 group compared to control group. Lactate levels were not significantly differed between control, 3,000, and 700 mg l−1 groups. There were no significant differences in serum levels of cholesterol, total protein, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, Na+, Cl, K+, and Ca2+. Results suggest that rapid anesthesia with higher dose is better than slow anesthesia with lower dose for blood sampling in Beluga.


Beluga Clove solution Stress Biochemical parameters Anesthesia 


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Seyyed Morteza Hoseini
    • 1
  • Seyed Abbas Hosseini
    • 1
  • Ali Jafar Nodeh
    • 1
  1. 1.Faculty of Fisheries and Environment, Department of FisheriesGorgan University of Agricultural Sciences and Natural ResourcesGorganIran

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