Fish Physiology and Biochemistry

, Volume 37, Issue 1, pp 177–186 | Cite as

Haemato-immunology and histo-architectural changes in Labeo rohita fingerlings: effect of dietary aflatoxin and mould inhibitor

  • S. Mohapatra
  • N. P. Sahu
  • A. K. Pal
  • A. K. Prusty
  • Vikas Kumar
  • Shivendra Kumar


A feeding trail of 60 days was conducted for delineating the effect of dietary aflatoxin (AFB1) with or without supplementation of a mixture of mould inhibitor (0.25% clove oil + 0.32% sodium propionate) on haematology, respiratory burst activity and histology of Labeo rohita fingerlings. Three hundred and sixty fishes (avg. wt. 1.48–1.54 g) were randomly distributed into eight treatment groups. Eight experimental diets with four different levels of aflatoxin (0, 10, 20 and 40 ppb) with or without mould inhibitor were prepared. Haematological parameters like total serum protein, albumin, globulin and A:G ratio were significantly (P < 0.05) reduced with increasing levels of aflatoxin in the diet. However, supplementation of mould inhibitor showed enhanced values when compared to non-supplemented counter parts suggesting ameliorating effects of mould inhibitor on aflatoxin. Total leucocyte count was higher in aflatoxin-treated groups. Histological observations were complementary to haematological parameters. Respiratory burst activity was significantly (P < 0.05) decreased in higher aflatoxin-treated groups but not affected significantly (P > 0.05) due to inclusion of inhibitor alone and/or interaction of aflatoxin level and inhibitor in the diet. From this study, it was concluded that up to 20 ppb aflatoxin level in the diet the haemato-immunological parameters are protected.


Aflatoxin Haematology Respiratory burst activity Histology Labeo rohita Clove oil Sodium propionate 



The authors are grateful to The Director, Central Institute of Fisheries Education, Mumbai, for providing facilities for carrying out the research work. The first author is grateful to Central Institute of Fisheries Education, Mumbai, for financial support by awarding Institutional Fellowship.


  1. Abo-Norag M, Edrington TS, Kubena LF, Harvey RB (1995) Influence of a hydrated sodium calcium aluminosilicate and virginiamycin on aflatoxicosis in broiler chickens. J Poult Sci 74:626–632Google Scholar
  2. Alpert M, Hutt MSR, Wogan NG, Davidson CS (1971) Association between aflatoxin content of food and hepatoma frequency in Uganda. Cancer 28:253–260PubMedCrossRefGoogle Scholar
  3. APHA (1998) Standard methods for the examination of water and wastewater. In: Clesceri LS, Greenberg AE, Eaton AD (eds) 20th edn. American Public Health Association, American Water Works Association, Water Environment Federation, Washington DCGoogle Scholar
  4. Ashley LM (1965) Histopathology of rainbow trout aflatoxicosis. In: Halver JE, Mitchel IA (eds) Trout research conference papers. U.S.D.H.E.W. and U.S.D. international fish/wildlife research report 70, pp 103–120Google Scholar
  5. Bullerman LB (1986) Mycotoxins and food safety. Food Technol 40:59–66Google Scholar
  6. CAST (Council for Agricultural Science and Technology) (1989) Mycotoxins: economic and health risks. Task force report 116. November 1989. Council for Agricultural Science and Technology, Ames, IAGoogle Scholar
  7. Chávez-Sánchez MC, Martínez Palacios CA, Osorio MorenoI (1994) Pathological effects of feeding young Oreochromis niloticus diets supplemented with different levels of aflatoxin B1. Aquaculture 127:49–60CrossRefGoogle Scholar
  8. Doumass BT, Watson W, Biggs HG (1971) Albumi standards and measurement of serum albumin with bromocressol green. Clinica Chimica Acta 31:87–96CrossRefGoogle Scholar
  9. El-Enbaawy M, Adel M, Marzouk MS, Salem AA (1994) The effects of acute and chronic aflatoxicosis on the immunological function of Oreochromis niloticus in Egyptian Veterinary Medical Journal Giza, pp 47–52Google Scholar
  10. El-Far F, Aziz NH, Hegazy S (1992) Inhibition by gamma-irradiation and anti microbial food additives of aflatoxin B1 production by Aspergillus flavus in poultry diet. Nahrung 36:143–149CrossRefGoogle Scholar
  11. Ghittino P (1976) Nutritional factors in trout hepatoma. Prog Exp Tumor Res 20:317–338PubMedGoogle Scholar
  12. Ghosh MK, Chhabra A, Atreja PP, Chopra RC (1996) Effect of treating with propionic acid, sodium bisulfite and sodium hydroxide on the biosynthesis of aflatoxin on groundnut cake. Anim Feed Sci Technol 60:43–49CrossRefGoogle Scholar
  13. Gowda NKS, Malathi V, Suganthi RU (2003) Screening for aflatoxin and effect of moisture, duration of storage and form of feed on fungal growth and toxin production in livestock feeds. Anim Nutr Feed Technol 3:45–51Google Scholar
  14. Haller RD, Roberts RJ (1980) Dual neoplasia in a specimen of Sarotherodon spiluris spiluris (Gunther) (Tilapia spiluris). J Fish Dis 3:63–66CrossRefGoogle Scholar
  15. Halver JE (1969) Aflatoxicosis and trout hepatoma. In: Goldblatt LA (ed) Aflatoxin: scientific background, control and implications. Academic Press, New York, pp 265–306Google Scholar
  16. Hendricks JD (1994) Carcinogenicity of aflatoxins in nonmammalian organisms. In: Eaton DL, Groopman JD (eds) Toxicology of aflatoxins: human health. Veterinary and agricultural significance. Academic Press, San Diego, pp 103–136Google Scholar
  17. Hendricks JD, Bailey GS (1989) Adventitious Toxins. In: Halver JE (ed) Fish nutrition, 2nd edn. Academic Press Inc., New York, USA, pp 605–651Google Scholar
  18. Hussein H, Brasel JM (2001) Toxicity, metabolism and impact of mycotoxins on humans and animals. Toxicology 167:101–134PubMedCrossRefGoogle Scholar
  19. IARC (1993) IARC Monographs on the evaluation of carcinogenic risks to humans. Some naturally occurring substances: food items, constituents, heterocyclic aromatic amines, mycotoxins. International agency for research on cancer, Lyon 56:249–395Google Scholar
  20. Jantrarotai W, Lovell RT (1990) Subchronic toxicity of dietary aflatoxin B1 to channel catfish. J Aquat Anim Health 2:248–256CrossRefGoogle Scholar
  21. Jindal N, Mahipal SK, Mahajan NK (1994) Toxicity of aflatoxin B1 in broiler chickens and its reduction by activated charcoal. Res Vet Sci 56:37–40PubMedCrossRefGoogle Scholar
  22. Kaneko JJ (1989) Serum proteins and the dysproteinemias. In: Kaneko JJ (ed) Clinical chemistry of domestic animals, 4th edn. Academic Press, San Diego, CAGoogle Scholar
  23. Kennedy D, Delaney KA, Koren G (1998) Mutagens, carcinogens and teratogens. In: Goldfrank L (ed) Goldfrank’s toxicologic emergencies, 6th edn. Appleton & Lange, Stanford, CT, pp 262–273Google Scholar
  24. Kubena LF, Harvey RB, Huff WE, Elissalde MH, Yersin AG, Phillips TD, Rottinghaus GE (1993) Efficacy of hydrated sodium calcium aluminosilicate to reduce the toxicity of aflatoxin and diacetoxyscirpenol. J Poult Sci 72:51–59Google Scholar
  25. Kurtz RS, Czuprynski CJ (1992) Effect of aflatoxin B1 on invitro production of interleukin-1 by bovine macrophages. Vet Immunol Immunopathol 34:149–158PubMedCrossRefGoogle Scholar
  26. Lovell T (1989) Non-nutient diet components. In: Lovell T (ed) Nutrition and feeding of fish. Van Nostrand Reinhold, New York, pp 93–105Google Scholar
  27. Marin ML, Murtha J, Dong W, Pestka JJ (1996) Effects of mycotoxins on cytokine production and proliferation in EL-4 thymoma cells. J Toxicol Environ Health 48:379–396PubMedCrossRefGoogle Scholar
  28. Reinhold JG (1953) Manual determination of serum total protein, albumin and globulin fractions by Biuret method. In: Reiner M (ed) Standard method of clinical chemistry. Academic Press, New York, p 88Google Scholar
  29. Roberts RJ (1989) Nutritional pathology of teleosts. In: Roberts RJ (ed) Fish pathology. London, Bailliere Tindall, p 337e62Google Scholar
  30. Sahoo PK, Mukherjee SC (2001) Immunosuppressive effects of aflatoxin B1 in Indian major carp (Labeo rohita). Comp Immunol Microbiol Infect Dis 24:143–149PubMedCrossRefGoogle Scholar
  31. Sahoo PK, Mukherjee SC (2002) Effect of dietary immunomodulation upon Edwardsiella tarda vaccination in healthy and immunocompromised Indian major carp (Labeo rohita). Fish Shellfish Immunol 12:1–16PubMedCrossRefGoogle Scholar
  32. Sahoo PK, Mukherjee SC, Jain AK, Mukherjee A (1998) Light and ultrastructural changes in opisthonephros of rohu, Labeo rohita during acute and subchronic aflatoxin B1 toxicity. In: International conference on fisheries and food security beyond the year 2000. Chiang Mai, ThailandGoogle Scholar
  33. Sato S, Matsushima N, Tanaka T, Sugimura T, Takashima F (1973) Hepatic tumors in the guppy (Lebistes reticulatus) induced by aflatoxin B1, dimethylnitrosamine and 2-acetaminofluorene. J Natl Cancer Inst 50:765–778Google Scholar
  34. Schreck CB (1996) Immunomodulation: Endogenous Factors. In: Iwama G, Nakanishi T (eds) The fish immune systems: organism. Pathogen and Environment, Academic Press, pp 311–337Google Scholar
  35. Secombs CJ (1990) Isolation of Salmonid macrophage and analysis of their killing activity. In: Stolen JSTC, Fletcher DP, Anderson BS, Van WB, Winkel M (eds) Techniques in fish immunology. SOS Publication, New Jersey, pp 137–152Google Scholar
  36. Stasiack AS, Bauman CP (1996) Neutrophil activity as a potential indicator immunomodulator Ecteinascida turbinata extract on Edwardsiella ictaluri infection of channel catfish. J Aquat Anim Health 7:141–146Google Scholar
  37. Van Vleet JF, Ferrans VJ (1992) Etiology factors and pathologic alterations in selenium-Vitamin E deficiency and excess in animals and humans. Biol Trace Elem Res 33:1–12PubMedCrossRefGoogle Scholar
  38. Verma SK (1997) Aflatoxin B1 in fish feeds and experimental acute aflatoxicosis in a teleost fish. J Aqua 5:13–21Google Scholar
  39. Weytes FAA, Cohen N, Flik G, Verburg-van-Kemenade BML (1999) Interaction between the immune system and the hypothalamo-pituitary-interrenal axis in fish. Fish Shellfish Immunol 9:1–20CrossRefGoogle Scholar
  40. Wiegertjes GF, Stet RJM, Parmentier HK, Muiswinkle WB (1996) Immunogenetics and disease resistance in fish: a comparative approach. Dev Comp Immunol 20:365–381PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • S. Mohapatra
    • 1
  • N. P. Sahu
    • 2
  • A. K. Pal
    • 2
  • A. K. Prusty
    • 1
  • Vikas Kumar
    • 3
  • Shivendra Kumar
    • 2
    • 4
  1. 1.Department of AquacultureCentral Institute of Fisheries EducationMumbaiIndia
  2. 2.Department of Fish Nutrition and BiochemistryCentral Institute of Fisheries EducationMumbaiIndia
  3. 3.Department of Aquaculture System and Animal Nutrition in the Tropics and Subtropics (480b)University of HohenheimStuttgartGermany
  4. 4.Krishi Vigyan KendraRajendra Agricultural UniversityW. ChamparanIndia

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