Fish Physiology and Biochemistry

, Volume 37, Issue 1, pp 1–20

Development, characterization, conservation and storage of fish cell lines: a review

Article

Abstract

Cell lines provide an important biological tool for carrying out investigations into physiology, virology, toxicology, carcinogenesis and transgenics. Teleost fish cell lines have been developed from a broad range of tissues such as ovary, fin, swim bladder, heart, spleen, liver, eye muscle, vertebrae, brain, skin. One hundred and twenty-four new fish cell lines from different fish species ranging from grouper to eel have been reported since the last review by Fryer and Lannan (J Tissue Culture Methods 16: 87–94, 1994). Among the cell lines listed, more than 60% were established from species from Asia, which contributes more than 80% of total fish production. This includes 59 cell lines from 19 freshwater, 54 from 22 marine and 11 from 3 brackish water fishes. Presently, about 283 cell lines have been established from finfish around the world. In addition to the listing and a scientific update on new cell lines, the importance of authentication, applications, cross-contamination and implications of overpassaged cell lines has also been discussed in this comprehensive review. The authors feel that the review will serve an updated database for beginners and established researchers in the field of fish cell line research and development.

Keywords

Fish Cell lines Development Characterization Conservation 

References

  1. Ahmed IVP, Chandra V, Parameswaran V, Venkatesan C, Shukla R, Bhonde RR, Hameed ASS (2008) A new epithelial-like cell line from eye muscle of catla (Catla catla): development and characterization. J Fish Biol 72:2026–2038CrossRefGoogle Scholar
  2. Ahmed IVP, Chandra V, Sudhakaran R, Rajesh Kumar S, Sarathi M, Sarath Babu V, Ramesh B, Sahul Hameed AS (2009a) Development and characterization of cell lines derived from rohu, Labeo rohita (Hamilton), and catla, Catla catla (Hamilton). J Fish Dis 32:211–218PubMedCrossRefGoogle Scholar
  3. Ahmed IVP, Sarath Babu V, Chandra V, Nambi KS, Thomas J, Ramesh B, Sahul Hameed AS (2009b) A new fibroblastic-like cell line from heart muscle of the Indian major carp (Catla catla): development and characterization. Aquaculture 293:180–186CrossRefGoogle Scholar
  4. Akimoto K, Takaoka T, Sorimachi K (2000) Development of a simple culture method for the tissues contaminated with microorganisms and application to establishment of a fish cell line. Zool Sci 17:61–63PubMedCrossRefGoogle Scholar
  5. Altman PL, Dittmer DS (1972) Biology data book, 2nd edn. Wiley, BethesdaGoogle Scholar
  6. Aviv A, Harley CB (2001) How long should telomeres be? Curr Hypertens Rep 3:145–151PubMedCrossRefGoogle Scholar
  7. Bahich H, Borenfreund E (1991) Cytotoxicity and genotoxicity assays with cultured fish cells: a review. Toxicol In Vitro 5:91–100CrossRefGoogle Scholar
  8. Barker KS, Quiniou SMA, Wilson MR, Bengten E, Stuge TB, Warr GW, Clem LW, Miller NW (2000) Telomerase expression and telomere length in immortal leukocyte lines from channel catfish. Dev Comp Immunol 24:583–595PubMedCrossRefGoogle Scholar
  9. Barnes D, Dowell L, Forest D, Parton A, Pavicevic P, Kazianis S (2006) Characterization of XM a novel Xiphophorus melanoma-derived cell line. Zebrafish 3:371–381PubMedCrossRefGoogle Scholar
  10. Bejar J, Borrego JJ, Alvarez MC (1997) A continuous cell line from the cultured marine fish gilt-head sea bream (Sparus aurata). Aquaculture 150:143–153CrossRefGoogle Scholar
  11. Bodnar AG, Ouellette M, Frolkis M, Holt SE, Shiu CP (1998) Extension of life-span by introduction of telomerase into normal human cells. Science 279:349–352PubMedCrossRefGoogle Scholar
  12. Bols NC, Lee LEJ (1991) Technology and uses of cell culture from tissues and organs of bony fish. Cytotechnology 6:163–187CrossRefGoogle Scholar
  13. Bols NC, Barlian AM, Chirino-Trejo M, Caldwell SJ, Goegan P, Lee LEJ (1994) Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills. J Fish Dis 17:601–611CrossRefGoogle Scholar
  14. Bols NC, Brubacher JL, Ganassin RC, Lee LEJ (2001) Ecotoxicology and innate immunity in fish. Dev Comp Immunol 25:853–873PubMedCrossRefGoogle Scholar
  15. Bryson SP, Joyce EM, Martell JD, Lee LEJ, Holt SE, Kales SE, Fujiki K, Dixon B, Bols NC (2006) A cell line (HEW) from embryos of haddock (Melanogrammus aeglefinius) and its capacity to tolerate environmental extremes. Mar Biotechnol 8:641–653PubMedCrossRefGoogle Scholar
  16. Buehring GC, Eby EA, Eby MJ (2004) Cell line cross-contamination: how aware are mammalian cell culturists of the problem and how to monitor it? In Vitro Cell Dev Biol Anim 40:211–215PubMedCrossRefGoogle Scholar
  17. Butler R, Nowak BF (2004) A dual enzyme method for the establishment of long and medium-term primary cultures of epithelial and fibroblastic cells from Atlantic salmon gills. J Fish Biol 65:1108–1125CrossRefGoogle Scholar
  18. Chang SF, Ngoh GH, Kueh LFS, Qin QM, Che CL, Lam TJ, Sin YM (2001) Development of a tropical marine fish cell line from Asian seabass (Lates calcarifer) for virus isolation. Aquaculture 192:133–145CrossRefGoogle Scholar
  19. Chen SL, Sha ZX, Ye HQ (2003a) Establishment of a pluripotent embryonic cell line from sea perch blastula embryo. Aquaculture 218:141–151CrossRefGoogle Scholar
  20. Chen SL, Ye HQ, Sha ZX, Hong Y (2003b) Derivation of a pluripotent embryonic cell line from red sea bream blastulas. J Fish Biol 63:795–805CrossRefGoogle Scholar
  21. Chen SL, Ren GC, Sha ZX, Shi CY (2004) Establishment of a continuous embryonic cell line from Japanese flounder Pararlichthys olivaceus for virus isolation. Dis Aquat Organ 60:241–246PubMedCrossRefGoogle Scholar
  22. Chen SL, Ren GC, Zhen-Xia S, Yunhan H (2005) Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus). Aquaculture 249:63–68CrossRefGoogle Scholar
  23. Chen MJ, Chiou PP, Liao YH, Lin CM, Chen TT (2010) Development and characterization of five rainbow trout pituitary single-cell clone lines capable of producing pituitary hormones. J Endocrinol 205:69–78PubMedCrossRefGoogle Scholar
  24. Chi SC, Hu WW, Lo BJ (1999) Establishment and characterization of a continuous cell line (GF-1) derived from grouper, Epinephelus coioides: a cell line susceptible to grouper nervous necrosis virus (GNNV). J Fish Dis 22:173–182CrossRefGoogle Scholar
  25. Ciba P, Schicktanz S, Anders E, Siegl E, Stielow A, Klink E, Kruse C (2008) Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney. Fish Physiol Biochem 34:367–372PubMedCrossRefGoogle Scholar
  26. Dannevig BH, Falk K, Namork E (1995) Isolation of the causal virus of infectious salmon anaemia (ISA) in a longterm cell line from Atlantic salmon head kidney. J Gen Virol 76:1353–1359PubMedCrossRefGoogle Scholar
  27. Devold M, Krossoy B, Asphaug V, Nylund A (2000) Use of RT-PCR for diagnosis of infectious salmon anaemia virus (ISAV) in carrier sea trout Salmo trutta after experimental infection. Dis Aquat Organ 40:9–18PubMedCrossRefGoogle Scholar
  28. DeWitte-Orr SJ, Lepic K, Bryson SP, Walsh SK, Lee LEJ, Bols NC (2006) Development of a continuous cell line, PBLE, from an American eel peripheral blood leukocyte preparation. In Vitro Cell Dev Biol Anim 42:263–272PubMedCrossRefGoogle Scholar
  29. Diago ML, López-Fierro P, Razquin B, Villena A (1995) Establishment and characterisation of a pronephric stromal cell line (TPS) from rainbow trout, Oncorhynchus mykiss. Fish Shellfish Immunol 5:441–457CrossRefGoogle Scholar
  30. Dong C, Weng S, Shi X, Shi N, He J (2008) Development of a mandarin fish Siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (ISKNV). Virus Res 135:273–281PubMedCrossRefGoogle Scholar
  31. Faisal M, Rutan BJ, Sami-Demmerle S (1995) Development of continuous liver cell cultures from the marine teleost, spot (Leiostomus xanthurus, Pisces: Sciaenidae). Aquaculture 132:59–72CrossRefGoogle Scholar
  32. Flano E, Loupez-Fierro P, Álvarez F, Razquin B, Villena A (1998) Splenic cultures from rainbow trout, Oncorhynchus mykiss: establishment and characterization. Fish Shellfish Immunol 8:589–606CrossRefGoogle Scholar
  33. Freshney RI (2005) Culture of animal cells: a manual of basic technique. Wiley, New JerseyCrossRefGoogle Scholar
  34. Fryer JL, Lannan CN (1994) Three decades of fish cell culture: a current listing of cell lines derived from fish. J Tissue Culture Methods 16:87–94CrossRefGoogle Scholar
  35. Ganassin RC, Bols NC (1998) Development of a monocyte/macrophage-like cell line, RTS11, from rainbow trout spleen. Fish Shellfish Immunol 8:457–476CrossRefGoogle Scholar
  36. Hameed ASS, Parameswaran V, Shukla R, Singh IS, Thirunavukkarasu AR, Bhonde RR (2006) Establishment and characterization of India’s first marine fish cell line from kidney of sea bass, Lates calcarifer. Aquaculture 257:92–103CrossRefGoogle Scholar
  37. Hayflick L, Moorehead PS (1961) The serial cultivation of human diploid cell strains. Exp Cell Res 25:585–621CrossRefGoogle Scholar
  38. Hightower LH, Renfro JL (1988) Recent applications of fish cell culture to biomedical research. J Exp Zool 248:290–302PubMedCrossRefGoogle Scholar
  39. Himizu C, Hike H, Denise MM, Breisch E, Westerman M, Buchanan J, Ligman HR, Phillips RB, Carlberg JM, Olst JV, Burns JC (2003) Characterization of a white bass (Morone Chrysops) embryonic cell line with epithelial features. In Vitro Cell Dev Biol Anim 39:29–35CrossRefGoogle Scholar
  40. Holen E, Kausland A, Skjærven K (2010) Embryonic stem cells isolated from Atlantic cod (Gadus morhua) and the developmental expression of a stage-specific transcription factor ac-Pou2. Fish Physiol Biochem. doi:10.1007/s10695-010-9381-z PubMedGoogle Scholar
  41. Hong Y, Winkler C, Schartl M (1996) Pluripotency and differentiation of embryonic stem cell lines from the medakafish (Oryzias latipes). Mech Dev 60:33–44PubMedCrossRefGoogle Scholar
  42. Hong Y, Chen S, Schartl M (2000) Embryonic stem cells in fish: current status and perspectives. Fish Physiol Biochem 22:165–170CrossRefGoogle Scholar
  43. Hsu YL, Yang YH, Chen YC, Tung MC, Wu JL, Engelking MH, Leong JC (1995) Development of an in vitro subculture system for the Oka organ lymphoid tissue of Penaeus monodon. Aquaculture 136:43–55CrossRefGoogle Scholar
  44. Huang X, Huang Y, Sun J, Han X, Qin Q (2009) Characterization of two grouper Epinephelus akaara cell lines: application to studies of Singapore grouper iridovirus (SGIV) propagation and virus–host interaction. Aquaculture 292:172–179CrossRefGoogle Scholar
  45. Hughes P, Marshall D, Reid Y, Parkes H, Gelber C (2007) The costs of using unauthenticated, over-passaged cell lines: how much more data do we need? Biotechniques 43:575–586PubMedCrossRefGoogle Scholar
  46. Imajoh M, Ikawa T, Oshima SI (2007) Characterization of a new fibroblast cell line from a tail fin of red sea bream, Pagrus major and phylogenetic relationships of a recent RSIV isolate in Japan. Virus Res 126:45–52PubMedCrossRefGoogle Scholar
  47. Iwamoto T, Nakai T, Mori K, Arimoto M, Furusawa I (2000) Cloning of the fish cell line SSN-1 for piscine nodaviruses. Dis Aquat Organ 43:81–89PubMedCrossRefGoogle Scholar
  48. Kang MS, Oh MJ, Kim YJ, Kawai K, Jung SJ (2003) Establishment and characterization of two cell lines derived from flounder, Paralichthys olivaceus (Temminck & Schlegel). J Fish Dis 26:657–665PubMedCrossRefGoogle Scholar
  49. Karunasagr I, Miller SD, Frerichs GN (1998) A new cell line from Puntius schwanenfeldi sensitive to snakehead fish cell line C-type retrovirus. Asian Fish Sci 8:151–157Google Scholar
  50. Katakura Y, Alam S, Shirahata S (1998) Immortalization by gene transfection. Method Cell Biol 57:69–91CrossRefGoogle Scholar
  51. Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA (1998) Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 396:84–88PubMedCrossRefGoogle Scholar
  52. Kou GH, Wang CH, Hung HW, Jng YS, Chou CM, Lo CF (1995) A cell line (EP-1) derived from “Beko disease” affected Japanese eel elver (Anguilla japonica) persistently infected with Pleistophors anguillarum. Aquaculture 132:161–173CrossRefGoogle Scholar
  53. Ku CC, Teng YC, Wang CS, Lu CH (2009) Establishment and characterization of three cell lines derived from the rockfish grouper Epinephelus quoyanus: Use for transgenic studies and cytotoxicity testing. Aquaculture 294:147–151CrossRefGoogle Scholar
  54. Kumar GS, Singh IBS, Philip R (2001) Development of a cell culture system from the ovarian tissue of African catfish (Clarias gariepinus). Aquaculture 194:51–62CrossRefGoogle Scholar
  55. Lai YS, Murali S, Ju HY, Wu MF, Guo IC, Chen SC, Fang K, Chang CY (2000) Two iridovirus-susceptible cell lines established from kidney and liver of grouper, Epinephelus awoara (Temminck and Schlegel), and partial characterization of grouper iridovirus. J Fish Dis 23:379–388CrossRefGoogle Scholar
  56. Lai YS, Murali S, Chiu HC, Ju HY, Lin YS, Chen SC, Guo IC, Fang K, Chang CY (2001) Propagation of yellow grouper nervous necrosis virus (YGNNV) in a new nodavirus susceptible cell line from yellow grouper, Epinephelus awoara (Temminck & Schlegel), brain tissue. J Fish Dis 24:299–309CrossRefGoogle Scholar
  57. Lai YS, John JAC, Lin CH, Guo IC, Chen SC, Fang F, Lin CH, Chang CY (2003) Establishment of cell lines from a tropical grouper, Epinephelus awoara (Temminck and Schlegel), and their susceptibility to grouper irido and nodaviruses. J Fish Dis 26:31–42PubMedCrossRefGoogle Scholar
  58. Lai YS, Chiou PP, Chen WJ, Chen YC, Chen CW, Chiu IS, Chen SD, Cheng YH, Chang CY (2008) Characterization of apoptosis induced by grouper iridovirus in two newly established cell lines from barramundi, Lates calcarifer (Bloch). J Fish Dis 31:825–834PubMedCrossRefGoogle Scholar
  59. Lakra WS (2010) Pluripotent embryonic stem cell line from catfish. ICAR News 16(1):3Google Scholar
  60. Lakra WS, Bhonde RR (1996) Development of primary cell culture from the caudal fin of an Indian major carp, Labeo rohita (Ham). Asian Fish Sci 9:149–152Google Scholar
  61. Lakra WS, Bhonde RR, Sivakumar N, Ayyappan S (2006a) A new fibroblast like cell line from the fry of golden mahseer Tor putitora (Ham). Aquaculture 253:238–243CrossRefGoogle Scholar
  62. Lakra WS, Sivakumar N, Goswami M, Bhonde RR (2006b) Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch). Aquacult Res 37:18–24CrossRefGoogle Scholar
  63. Lakra WS, Swaminathan TR, Rathore G, Goswami M, Yadav K, Kapoor S (2010) Development and characterization of three new diploid cell lines from Labeo rohita (Ham.). Biotechnol Progr. doi:10.1002/btpr.418 Google Scholar
  64. Lannan CN (1994) Fish cell culture: a protocol for quality control. J Tissue Cult Methods 16:95–98CrossRefGoogle Scholar
  65. Lannan CN, Winton JR, Fryer JL (1984) Fish cell lines: establishment and characterization of nine cell lines from salmonids. In Vitro 20:671–676PubMedCrossRefGoogle Scholar
  66. Lee LEJ, Caldwell SJ, Gibbons J (1997) Development of a cell line from skin of goldfish, Carassius auratus, and effects of ascorbic acid on collagen deposition. Histochem J 29:31–43PubMedCrossRefGoogle Scholar
  67. Leibovitz A (1963) The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg 78:173–180PubMedGoogle Scholar
  68. Luc Rougée GK, Ostrander RH, Richmond YL (2007) Establishment, characterization, and viral susceptibility of two cell lines derived from goldfish Carassius auratus muscle and swim bladder. Dis Aquat Organ 77:127–135PubMedCrossRefGoogle Scholar
  69. Matsuo Y, Nishizaki C, Drexler HG (1999) Efficient DNA fingerprinting method for the identification of cross-culture contamination of cell lines. Hum Cell 12:149–154PubMedGoogle Scholar
  70. Miller NW, Chinchar VG, Clem LW (1994) Development of leukocyte cell lines from the channel catfish (Ictalurus punctatus). J Tissue Cult Methods 16:117–123CrossRefGoogle Scholar
  71. Ossum CG, Hoffmann EK, Vijayan MM, Holt SE, Bols NC (2004) Characterization of a novel fibroblast-like cell line from rainbow trout and responses to sublethal anoxia. J Fish Biol 64:1103–1116CrossRefGoogle Scholar
  72. Ostrander GK, Blair JB, Everlya SG, Marley AW, Bales ER, Obertw Veltri D, Avide Hinton M, Arko Kihiro LO, Hawkins EW (1995) Long-term primary culture of epithelial cells from rainbow trout (Oncorhynchus mykiss) liver. In Vitro Cell Dev Biol Anim 31:367–378PubMedCrossRefGoogle Scholar
  73. Parameswaran V, Shukla R, Bhonde RR, Hameed ASS (2006a) Establishment of embryonic cell line from sea bass (Lates calcarifer) for virus isolation. J Virol Methods 137:309–316PubMedCrossRefGoogle Scholar
  74. Parameswaran V, Shukla R, Bhonde RR, Hameed ASS (2006b) Splenic cell line from sea bass, Lates calcarifer: establishment and characterization. Aquaculture 261:43–53CrossRefGoogle Scholar
  75. Parameswaran V, Shukla R, Bhonde RR, Hameed ASS (2006c) Development of a Pluripotent ES-like cell Line from Asian sea bass (Lates calcarifer)—an oviparous stem cell line mimicking viviparous ES cells. Mar Biotechnol 9:766–775CrossRefGoogle Scholar
  76. Parameswaran V, Ahmed VPI, Shukla R, Bhonde RR, Hameed ASS (2007) Development and characterization of two new cell lines from milkfish (Chanos chanos) and grouper (Epinephelus coioides) for virus isolation. Mar Biotechnol 9:281–291PubMedCrossRefGoogle Scholar
  77. Parodi B, Aresu O, Bini D, Lorenzini R (2002) Species identification and confirmation of human and animal cell lines: a PCR-based method. Biotechniques 32:432–440PubMedGoogle Scholar
  78. Perry GML, McDonald GJ, Ferguson MM, Ganassin RC, Bols NC (2001) Characterization of rainbow trout cell lines using microsatellite DNA profiling. Cytotechnology 37:143–151PubMedCrossRefGoogle Scholar
  79. Pombinho AR, Laize V, Molha DM, Marques SMP, Cancela LM (2004) Development of two bone-derived cell lines from the marine teleost Sparus aurata; evidence for extra cellular matrix mineralization and cell-type-specific expression of matrix Gla protein and osteocalcin. Cell Tissue Res 315:393–406PubMedCrossRefGoogle Scholar
  80. Qin QW, Wu TH, Jia TL, Hegde A, Zhang RQ (2006) Development and characterization of a new tropical marine fish cell line from grouper, Epinephelus coioides susceptible to iridovirus and nodavirus. J Virol Methods 131:58–64PubMedCrossRefGoogle Scholar
  81. Ristow SS, De Avila J (1994) Susceptibility of four salmonids cell lines to infectious hematopoietic necrosis virus. J Aquat Anim Health 6:260–265CrossRefGoogle Scholar
  82. Ristow SS, Thorgaard GH (1998) Development of long term cell lines from homozygous clones of rainbow trout. J Aquat Anim Health 10:75–82CrossRefGoogle Scholar
  83. Ryan LA, Seymour CB, O’Neill-Mehlenbacher A, Mothersill CE (2008) Radiation-induced adaptive response in fish cell lines. J Environ Radioact 99:739–747PubMedCrossRefGoogle Scholar
  84. Sathe PS, Maurya DT, Basu A, Gogate SS, Banerjee K (1995) Establishment and characterization of a new fish cell line, MG-3, from the gills of mrigal Cirrhinus mrigala. Ind J Exp Biol 33:589–594Google Scholar
  85. Sathe PS, Basu A, Mourya DT, Marathe BA, Gogate SS, Banerjee K (1997) A cell line from the gill tissues of Indian cyprinoid Labeo rohita. In Vitro Cell Dev Biol Anim 33:425–427PubMedCrossRefGoogle Scholar
  86. Sato G (2008) Tissue cultures: the unrealized potential. Cytotechnology 57:111–114PubMedCrossRefGoogle Scholar
  87. Schirmer K (2006) Proposal to improve vertebrate cell cultures to establish them as substitutes for the regulatory testing of chemicals and effluents using fish. Toxicology 224:163–183PubMedCrossRefGoogle Scholar
  88. Servili A, Bufalino MR, Nishikawa R, de Melo IS, Muñoz-Cueto JA, Lee LEJ (2009) Establishment of long term cultures of neural stem cells from adult sea bass, Dicentrarchus labrax. Comp Biochem Physiol Part A 152:245–254CrossRefGoogle Scholar
  89. Sha ZA, Ren G, Wang X, Wang N, Chen S (2010) Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis). Acta Oceanologica Sinica 29(2):81–87CrossRefGoogle Scholar
  90. Sun L, Bradford CS, Ghosh C, Collodi P, Barnes DW (1995) ES-like cell cultures derived from early zebrafish embryos. Mol Mar Biol Biotech 4:193–199Google Scholar
  91. Swaminathan TR, Lakra WS, Gopalakrishnan A, Basheer VS, Khushwaha B, Sajeela KA (2010) Development and characterization of a new epithelial cell line PSF from caudal fin of Green chromide, Etroplus suratensis (Bloch, 1790). In Vitro Cell Dev Biol Anim. doi:10.1007/s11626-010-9326-y PubMedGoogle Scholar
  92. Takakura M, Kyo S, Kanaya T, Hirano H, Takeda J, Yutsudo M, Inoue M (1999) Cloning of human telomerase catalytic subunit (hTERT) gene promoter and identification of proximal core promoter sequences essential for transcriptional activation in immortalized and cancer cells. Cancer Res 59:551–557PubMedGoogle Scholar
  93. Tokiwa T, Kusaka Y, Muraoka A, Sato J (1989) Examination of HeLa cell contamination of human cell lines derived from primary hepatomas using glucose-6-phosphate dehydrogenase and lactate dehydrogenase isozymes. Acta medica Okayama 43:245–247PubMedGoogle Scholar
  94. Tong SL, Lee H, Miao HZ (1997) The establishment and partial characterization of a continuous fish cell line FG-9307 from the gill of flounder. Paralichthys olivaceus Aquaculture 156:327–333CrossRefGoogle Scholar
  95. Tong SL, Miao HZ, Li H (1998) Three new continuous fish cell lines of SPH, SPS and RSBF derived from sea perch (Lateolabrax japaonicus) and red sea bream (Pagrosomus major). Aquaculture 169:143–151CrossRefGoogle Scholar
  96. Villena AJ (2003) Applications and needs of fish and shellfish cell culture for disease control in aquaculture. Rev Fish Biol Fisher 13:111–140CrossRefGoogle Scholar
  97. Wagg SK, Lee LEJ (2005) A proteomics approach to identifying fish cell lines. Proteomics 5:4236–4244PubMedCrossRefGoogle Scholar
  98. Wang R, Neumann NF, Shen Q, Belosevic M (1995) Establishment and characterization of a macrophage cell line from the goldfish. Fish Shellfish Immunol 5:329–346CrossRefGoogle Scholar
  99. Wang G, LaPatra S, Zeng L, Zhao Z, Lu Y (2003) Establishment, growth, cryopreservation and species of origin identification of three cell lines from white sturgeon, Acipenser transmontanus. Methods Cell Sci 25:211–220PubMedCrossRefGoogle Scholar
  100. Wang XL, Wang N, Sha ZX, Chen SL (2010a) Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis). Fish Physiol Biochem. doi:10.1007/s10695-010-9396-5 Google Scholar
  101. Wang N, Wang XL, Sha ZX, Tian YS, Chen SL (2010b) Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus). Fish Physiol Biochem. doi:10.1007/s10695-010-9402-y Google Scholar
  102. Watanabe T, Nakano M, Asakawa H, Moritomo T (1987) Cell culture of rainbow trout liver. B Jpn Soc Sci Fish 53:537–542Google Scholar
  103. Wen CM, Lee CW, Wang CS, Cheng YH, Huang HY (2008) Development of two cell lines from Epinephelus coioides brain tissue for characterization of betanodavirus and megalocytivirus infectivity and propagation. Aquaculture 278:14–21CrossRefGoogle Scholar
  104. Wenger SL, Senft JR, Sargent LM, Bamezai R, Bairwa N, Grant SG (2004) Comparison of established cell lines at different passages by karyotype and comparative genomic hybridization. Biosci Rep 24:631–639PubMedCrossRefGoogle Scholar
  105. Wergeland HI, Jakobsen RA (2001) A salmonid cell line (TO) for production of infectious salmon anaemia virus (ISAV). Dis Aquat Organ 44:183–190PubMedCrossRefGoogle Scholar
  106. Williams LM, Crane MStJ, Gudkovs N (2003) Development and characterisation of pilchard (Sardinops sagax neopilchardus) cell lines derived from liver and heart tissues. Methods Cell Sci 25:105–113PubMedCrossRefGoogle Scholar
  107. Wolf K (1988) Fish viruses and fish viral diseases. Cornell University Press, New YorkGoogle Scholar
  108. Wolf K, Mann JA (1980) Poikilotherm vertebrate cell lines and viruses: a current listing for fishes. In Vitro 16:168–179PubMedCrossRefGoogle Scholar
  109. Wolf K, Quimby MC (1962) Established eurythermic line of fish cells in vitro. Science 135:1065–1066PubMedCrossRefGoogle Scholar
  110. Wolf K, Quimby MC (1966) Fish cell and tissue culture. In: Hoar WS, Randall DJ (eds) Fish physiology, vol III. Academic Press, New York, pp 253–305Google Scholar
  111. Wolf K, Quimby C (1976) Primary monolayer culture of fish cells initiated from trypsinized tissues. TCA Manual 2:453–456CrossRefGoogle Scholar
  112. Ye HQ, Chen SL, Sha ZX, Xu MY (2006) Development and characterization of cell lines from heart, liver, spleen and head kidney of sea perch Lateolabrax japonicus. J Fish Biol 69:115–126CrossRefGoogle Scholar
  113. Yu H, Cook TJ, Sinko PJ (1997) Evidence for diminished functional expression of intestinal transporters in Caco-2 cell monolayers at high passages. Pharm Res 14:757–762PubMedCrossRefGoogle Scholar
  114. Zhao Z, Lu Y (2006) Establishment and characterization of two cell lines from bluefin trevally Caranx melampygus. Dis Aquat Organ 68:91–100PubMedCrossRefGoogle Scholar
  115. Zhao Y, Glesne D, Huberman E (2003) A human peripheral blood monocyte derived subset acts as pluripotent stem cells. Proc Natl Acad Sci 100:2426–2431PubMedCrossRefGoogle Scholar
  116. Zhou GZ, Li ZQ, Yuan XP, Zhang QY (2007) Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara). Mar Biotechnol 9:370–376PubMedCrossRefGoogle Scholar
  117. Zhou GZ, Gui L, Li ZQ, Yuan XP, Zhang QY (2008) Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus. J Fish Biol 73:2058–2067CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • W. S. Lakra
    • 1
  • T. Raja Swaminathan
    • 1
  • K. P. Joy
    • 1
    • 2
  1. 1.National Bureau of Fish Genetic ResourcesLucknowIndia
  2. 2.Department of ZoologyBanaras Hindu UniversityVaranasiIndia

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