Fish Physiology and Biochemistry

, Volume 36, Issue 4, pp 1181–1189 | Cite as

Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis)

  • X. L. Wang
  • N. Wang
  • Z. X. Sha
  • S. L. Chen


A new cell line was established from the heart of a cultured marine fish, half smooth tongue sole (Cynoglossus semilaevis), designated as CSH (Cynoglossus semilaevis heart cell line). The CSH cells grow over 400 days in minimum essential medium (MEM) supplemented with 10% fetal bovine serum (FBS) and 2 ng/ml basic fibroblast growth factor (bFGF). The suitable temperature for the cell growth was 24–30°C with the optimum growth at 24°C and a reduced growth at 12 and 30°C. FBS and bFGF concentration were the two important components for CSH cells proliferation. Twenty percent FBS in the medium was found to be the optimum concentration and bFGF promoted the growth of CSH cells. The double time of the cells at 24°C was determined to 73.39 h. Chromosome analysis revealed that 44% of the cells maintained a normal diploid chromosome number (2n = 42) in the CSH cells at Passage 58. The fluorescent signals were observed in CSH after the cells were transfected with green fluorescent protein (GFP) reporter plasmids. CSH cells showed the cytopathic effect (CPE) after infection with lymphosystis disease virus (LCDV). Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy, and a segment of MCP gene for major capsid protein of LCDV was found by PCR amplification DNA of virus-infected cells.


Cell line Half smooth tongue sole (Cynoglossus semilaevisCSH Karyotype GFP LCDV 



This work was supported by grants from State 863 High-Technology R&D Project of China (2006AA09Z406, 2006AA10A401), and Taishan Scholar Project of Shandong Province.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • X. L. Wang
    • 1
    • 2
  • N. Wang
    • 1
  • Z. X. Sha
    • 1
  • S. L. Chen
    • 1
  1. 1.Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoChina
  2. 2.College of Marine Life ScienceOcean University of ChinaQingdaoChina

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