Anesthesia induces stress in Atlantic salmon (Salmo salar), Atlantic cod (Gadus morhua) and Atlantic halibut (Hippoglossus hippoglossus)
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Stress in response to anesthesia with benzocaine, MS-222, metomidate and isoeugenol was studied in Atlantic salmon (Salmo salar), Atlantic halibut (Hippoglossus hippoglossus), and Atlantic cod (Gadus morhua) with no concomitant stress from handling or confinement in association with anesthesia or sampling. All of the anesthetics tested induced a stress response in all species, displayed by a release of cortisol to the water. MS-222 anesthesia elicited the highest cortisol release rates, reaching maximum levels 0.5 h post-exposure and returning to basal levels after 3–4 h. Benzocaine anesthesia caused a bimodal response where the initial peak in cortisol release rate was followed by a second increase lasting towards the end of the trial (6 h). This bimodality was more profound in Atlantic salmon than in Atlantic halibut and Atlantic cod. Metomidate anesthesia induced the lowest release of cortisol of the agents tested in both Atlantic halibut and Atlantic cod, but resulted in a bimodal response in Atlantic salmon where the initial increase in cortisol release was followed by a larger increase peaking at 2–2.5 h post exposure before returning to basal levels after 5 h. The stress induced in Atlantic salmon by isoeugenol anesthesia resembled that of MS-222, but did not reach the same elevated level. Overall, the cortisol release was most profound in Atlantic salmon followed by Atlantic halibut and Atlantic cod.
KeywordsAnesthesia Stress Cortisol MS-222 Benzocaine Metomidate Isoeugenol
The authors would like to thank the staff of the Institute of Marine Research, and in particular Ivar Helge Matre and Rina Helen Skoglund for their valuable technical assistance, and Grethe Thorsheim for skilful help and support with water sample processing and analysis. Ole Folkedal and Thomas Torgersen are highly acknowledged for proficient support in calculating cortisol release rates. Financial support was given by the Norwegian Research Council, grant no. 152898/120.
- Ackerman PA, Morgan JD, Iwama GK (2005) Anesthetics. CCAC guidelines on: the care and use of fish in research, teaching and testing. Canadian Council on Animal Care, Ottawa. Available at: http://www.ccac.ca/en/CCAC_Programs/Guidelines_Policies/GDLINES/Fish/Fish%20Anesthetics%20-%20ENG.pdf Accessed 10 Feb 2009
- Donaldson EM (1981) The pituitary-interrenal axis as an indicator of stress in fish. In: Pickering AD (ed) Stress and Fish. Academic, London, pp 11–47Google Scholar
- Hunn JB, Allen JL (1974) Movement of drugs across gills of fishes. Annu Rev Pharmacol Toxicol 14:47–55Google Scholar
- King WV, Hooper B, Hillsgrove S, Benton C, Berlinsky DL (2005) The use of clove oil, metomidate, tricaine methanesulphonate and 2-phenoxyethanol for inducing anaesthesia and their effect on the cortisol stress response in black sea bass (Centropristis striata L.). Aquac Res 36:1442–1449CrossRefGoogle Scholar
- Kreiberg H, Powell J (1991) Metomidate sedation reduces handling stress in chinook salmon. World Aquac 22:58–59Google Scholar
- Mazeaud MM, Mazeaud F (1981) Adrenergic responses to stress in fish. In: Pickering AD (ed) Stress and Fish. Academic, London, pp 49–75Google Scholar
- Rang HP, Dale MM, Ritter JM, Moore PK (2003) Pharmacology. Churchill Livingstone, LondonGoogle Scholar
- Scott AP, Hirschenhauser K, Bender N, Oliveira R, Earley RL, Sebire M, Ellis T, Pavlidis M, Hubbard PC, Huertas M, Canario A (2008) Non-invasive measurement of steroids in fish-holding water: important considerations when applying the procedure to behaviour studies. Behaviour 145:1307–1328CrossRefGoogle Scholar
- Sorensen PW, Scott AP (1994) The evolution of hormonal sex-pheromones in teleost fish–poor correlation between the pattern of steroid release by goldfish and olfactory sensitivity suggests that these cues evolved as a result of chemical spying rather than signal specialization. Acta Physiol Scand 152:191–205CrossRefPubMedGoogle Scholar