Fish Physiology and Biochemistry

, Volume 36, Issue 1, pp 39–43 | Cite as

Determination of normal values of some blood serum enzymes in Acipenser stellatus Pallas

  • D. Shahsavani
  • M. Mohri
  • H. Gholipour KananiEmail author


Hematological studies on fishes have assumed greater significance due to the increasing emphasis on pisciculture and greater awareness of the pollution of natural water resources. Such studies have generally been used as an effective and sensitive index to monitor physiological and pathological changes in fishes, especially in the management of endangered species. This study was undertaken to establish a reference range for six important blood serum enzymes. Serum samples of 40 Acipenser stellatus (20 female and 20 male) were analyzed, and serum enzyme values were determined. The reference ranges for the total samples and also for each sex were determined. The activities of measured enzymes in serum were: aspartate aminotransferase (AST) 265.60 ± 56.55 IU/l, alanine aminotransferase (ALT) 5.65 ± 1.18 IU/l, acid phosphatase (ACP) 15.63 ± 2.59 IU/l, lactate dehydrogenase (LDH) 2007.15 ± 521.97 IU/l, creatine kinase (CK) 6,596.05 ± 1,807.19 IU/l, and alkaline phosphatase (ALP) 69.05 ± 13.04 IU/l. There were significant differences between male and female fish in terms of AST and CK activity (P < 0.05). These may be related to the season of sampling and changing physiological cycles during spawning during which period the sexual hormones and stress are more obvious. However, there were no differences in the activity of ALP, ACP, LDH, and ALT between sexes. The correlations between measured enzymes were also determined.


Blood parameters Enzyme Sturgeon 


  1. Aldrin JF, Messager F, Laurencin B (1982) La biochimie clinique en aquaculture. Internet et perspectives. CNEXO. Actes Colloq 14:291–326Google Scholar
  2. Anver CE (2004) Blood chemistry (electrolytes, lipoprotein and enzymes) values of black scorpion fish (Scorpaena porcus, 1758) in the Dardnelles, Turkey. J Biol Sci 4:716–719CrossRefGoogle Scholar
  3. Asadi F, Masoudifard M, Vajhi A, Lee K, Pourkabir M, Khazraeinia P (2006) Serum biochemical parameters of Acipenser persicus. Fish Physiol Biochem 32:43–47CrossRefPubMedGoogle Scholar
  4. Baker DW, Wood AM, Litvak MK, Kieffer JD (2005) Hematology of juvenile Acipenser oxyrinchus and Acipenser brevirostrum at rest following forced activity. J Fish Biol 66:208–221. doi: 10.1111/j.0022-1112.2005.00595.x CrossRefGoogle Scholar
  5. Bentinck-Smith J, Beleau MH, Waterstart PR, Tucker CS, Stile F, Bowser PR, Brown LA (1987) Biochemistry references range for commercially reared channel catfish. Prog Fish Cult lnt 49:108–114CrossRefGoogle Scholar
  6. Brenden RA, Huizinga HW (1986) Pathophysiology of experimental Aeromonas hydrophila infection gold fish (Carassius auratus). J Fish Dis 9:163–167. doi: 10.1111/j.1365-2761.1986.tb00999.x CrossRefGoogle Scholar
  7. Bouck RG, Ball RC (1966) Influence of capture methods on blood characteristics and mortality in rainbow trout (Salmo gairdneri). Trans Am Fish Soc 95:167–176. doi: 10.1577/1548-8659(1966)95[170:IOCMOB]2.0.CO;2 CrossRefGoogle Scholar
  8. Casillas E, Sundquist J, Ames WE et al (1982) Optimization of assay condition for, and the selected tissue distribution of alanine amino transferase and aspartate amino transferase of English sole (Paraphrys ventulus). J Fish Biol 27:197–204. doi: 10.1111/j.1095-8649.1982.tb03999.x CrossRefGoogle Scholar
  9. Evenberg D, de Graaff P, Fleuren W, van Muiswinkel WB (1986) Blood changes in carp (cyprinus carpio) induced by ulcerative Aeromonas salmonicida infections. Vet immunol Immunopathol 12:321–330CrossRefPubMedGoogle Scholar
  10. Hille S (1982) A literature review of the blood chemistry of rainbow trout (Salmo gairdneri). J Fish Biol 20:535–569CrossRefGoogle Scholar
  11. Hine DM, Wain JM (1985) The enzyme cytochemistry and composition of elasmobranches granulocytes. J Fish Biol 30:465–475. doi: 10.1111/j.1095-8649.1987.tb05770.x CrossRefGoogle Scholar
  12. Luskova V (1997) Annual cycles and normal values of hematological parameters in fishes. Acta Sci Nat Brno 31:1–70Google Scholar
  13. Moghim M, Vajhi AR, Veshkini A, Masoudifard M (2002) Determination of sex and maturity in Acipenser stellatus by using ultrasonography. J Appl Ichthyol 18:325–328. doi: 10.1046/j.1439-0426.2002.00423.x CrossRefGoogle Scholar
  14. Sakamoto K, Lewbart GA, Smith TM (2001) Blood chemistry values of juvenile Red pacu (Piaractus brachypomus). Vet Clin Pathol 30:50–52. doi: 10.1111/j.1939-165X.2001.tb00257.x CrossRefPubMedGoogle Scholar
  15. Shahsavani D, Mohri M, Taghvaiemoghadam E (2007) Determination of concentration of some blood serum enzymes in Huso huso. J Vet Res Tehran Univ 62:127–129Google Scholar
  16. Smith CG, Lewis WM, Kaplan HM (1952) A comparative morphologic and physiologic study of fish blood. Prog Fish-Cult 14:169–172. doi: 10.1577/1548-8640(1952)14[169:ACMAPS]2.0.CO;2 CrossRefGoogle Scholar
  17. Speer L, Lauck L, Pikitch E, Boa S, Dropkin L, Spruill V (2000) Roe to ruin: the decline of sturgeon in the Caspian Sea and the road to recovery. Available at: Cited Dec 2000
  18. Stoskopf MK (ed) (1993) Fish medicine. W.B. Saunders Company, Philadelphia, xix + 882 ppGoogle Scholar
  19. Wagner T, Congleton JL (2004) Blood chemistry correlates of nutritional condition, tissue damage, and stress in migrating juvenile Chinook salmon (Onchorhynchus tshawytscha). Can J Fish Aquat Sci 61:1066–1074. doi: 10.1139/f04-050 CrossRefGoogle Scholar
  20. Warner MC, Dieh SA (1978) Dilution and temperature of analysis on blood serum values in rainbow trout (Salmo gairdneri). J Fish Biol 13:315–319. doi: 10.1111/j.1095-8649.1978.tb03439.x CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Food Hygiene and Aquaculture, School of Veterinary MedicineFerdowsi University of MashhadMashhadIran
  2. 2.Department of Clinical Science, School of Veterinary MedicineFerdowsi University of MashhadMashhadIran
  3. 3.School of Veterinary MedicineFerdowsi University of MashhadMashhadIran

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