Fish Physiology and Biochemistry

, Volume 35, Issue 3, pp 435–441 | Cite as

Comparison of haematology and serum biochemistry of cultured and wild Dojo loach Misgurnus anguillicaudatus

  • Xiaoyun Zhou
  • Mingyun Li
  • Khalid Abbas
  • Weimin Wang


Haematological and serum biochemistry parameters were studied and compared between cultured and wild ecotypes of Dojo loach Misgurnus anguillicaudatus during the prime fishing season, i.e. May–August. Data were analysed for the impact of feeding regime and other ecological conditions on the physiology of fish. The results revealed that haemoglobin, cholesterol, total protein, creatinine and uric acid levels in the two ecotypes were significantly different (n = 56, df = 54, P < 0.05). In addition, red blood cell, glucose, triglyceride and urea nitrogen levels were significantly higher in cultured individuals (n = 56, df = 54, < 0.01) than in their wild counterparts. In contrast, the white blood cell level in cultured fish was significantly (n = 56, df = 54, P < 0.01) lower than that in the wild ones. These differences can be attributed to the physiological acclimatization of the fish to their living conditions and feeding regime, which influences the energy metabolism and, consequently, the health of the fish.


Cultured and wild Haematology Misgurnus anguillicaudatus Serum biochemistry 



This research is funded by “National Key Technology R and D Program in Eleventh-Five-Year Plan” entitled as “High quality and high efficiency freshwater aquaculture technology research program” (accession no.: 2006BAD03B07) and “The integrated demonstration of safe fishery production”(accession no.: 2006BAK02A22). This research is also a component of the AquaFish-ACRSP (Aquaculture Collaborative Research Support Program), supported by the US Agency for International Development (USAID).


  1. Allen FM, Patrick JW, Roger TH (2005) Blood biochemistry of the oyster toadfish. J Aquat Anim Health 17:170–176CrossRefGoogle Scholar
  2. Arends RJ, Mancera JM, Munoz JL, Wendelaar Bonga SE, Flik G (1999) The stress response of the gilthead sea bream (Sparus aurata L.) to air exposure and confinement. J Endocrinol 163:149–157PubMedCrossRefGoogle Scholar
  3. Artacho P, Soto-Gamboa M, Verdugo C, Nespolo RF (2007) Blood biochemistry reveals malnutrition in black-necked swans (Cygnus melanocoryphus) living in a conservation priority area. Comp Biochem Physiol A 146:283–290CrossRefGoogle Scholar
  4. Bahmani M, Kazemi R, Donskaya P (2001) A comparative study of some hematological features in young reared sturgeons (Acipenser persicus and Huso huso). Fish Physiol Biochem 24:135–140CrossRefGoogle Scholar
  5. Burtis CA, Ashwood ER (1996) Tietz fundamentals of clinical chemistry. WB Saunders, PhiladelphiaGoogle Scholar
  6. Cech JJ, Bartholow SD, Young PS, Hopkins TE (1996) Stripped bass exercise and handling stress in freshwater: physiological responses to recovery environment. Trans Am Fish Soc 125:308–320CrossRefGoogle Scholar
  7. Chen YE, Jin S, Wang GL (2005) Study on blood physiological and biochemical indices of Vibrio alginilyticus disease of Lateolabrax japonicas. J Oceanogr Taiwan Strait 24:104–108Google Scholar
  8. Christofilogiannis P (1993) The veterinary approach to sea bass and sea bream. In: Brown L (ed) Aquaculture for veterinarians. Pergamon Press, Oxford, pp 379–395Google Scholar
  9. Cnaani A, Tinman S, Avidar Y, Ron M, Hulata G (2004) Comparative study of biochemical parameters in response to stress in O.aureus, O.mossambicus and two strains of O.niloticus. Aquacult Res 35:1434–1440CrossRefGoogle Scholar
  10. Coz-Rakovac R, Strunjak-perovic I, Hacmanjek M, Topic PN, Lipej Z, Sostaric B (2005) Blood chemistry and histological properties of wild and cultured sea bass (Dicentrarchus labrax) in the north Adriatic Sea. Vet Res Commun 29:677–687PubMedCrossRefGoogle Scholar
  11. Domezain A, Garcia-Gallego M, Domezain J, Sanz A (1997) Evolution during growth of the biometry and the blood constants of the sturgeon, Acipenser naccarii. In: Proc 3rd Int Symp Sturgeons, 4–8 July, Piacenza, ItalyGoogle Scholar
  12. Drabkin DL, Austin JH (1935) Spectrophotometric studies, II. Preparation from washed blood cells; nitric oxide haemoglobin and sulphaemoglobin. J Biol Chem 112:51–65Google Scholar
  13. Gao ZX, Wang WM, Abbas K, Zhou XY, Yang Y, Diana JS, Wang HP, Wang HL, Li Y, Sun YH (2007) Haematological characterization of loach Misgurnus anguillicaudatus: comparison among diploid, triploid and tetraploid specimens. Comp Biochem Physiol A 147:1001–1008CrossRefGoogle Scholar
  14. Grant GH, Silverman LM, Christenson RH (1987) Amino acids and proteins. In: Tietz NZ (ed) Fundamentals of clinical chemistry, 3rd edn. WB Saunders, Philadelphia, pp 291–345Google Scholar
  15. Hardy D, Audet C (1990) Evaluation of plasma glucose as an indicator of mild chronic stress in brook charr (Salvelinus fontinalis). Bull Aquacult Assoc Can 90:54–56Google Scholar
  16. Houston AH (1990) Blood and circulation. In: Moyle (ed) Methods for fish biology. Am Fish Soc 273–334Google Scholar
  17. Kubokawa K, Watanabe T, Yoshioka M, Iwata M (1999) Effects of acute stress on plasma cortisol, sex steroid hormone and glucose levels in male and female sockeye salmon during the breeding season. Aquaculture 172:335–349CrossRefGoogle Scholar
  18. Lermen CL, Lappe R, Crestani M, Vieira VP, Gioda CR, Schetinger MRC, Baldisserotto B, Moraes G, Morsch VM (2004) Effect of different temperature regimes on metabolic and blood parameters of silver catfish Rhamdia quelen. Aquaculture 239:497–507CrossRefGoogle Scholar
  19. Man SH, Hodgkiss IJ (1981) Hong Kong freshwater fishes. Urban Council Publ, Hong KongGoogle Scholar
  20. Mckenzie S, Deane EM, Burnett L (2002) Haematology and serum biochemistry of the Tammar Wallaby, Macropus eugenii. Comp Clin Pathol 11:229–237CrossRefGoogle Scholar
  21. Morris MW, Davey FR (1996) Basic examination of blood. In: Henry JB (ed) Clinical diagnosis and management by laboratory methods, 19th edn. WB Saunders, Philadelphia, USA, pp 549–593Google Scholar
  22. Nespolo RF, Rosenmann M (2002) Intraspecific allometry of haematological parameter in Basilichthys australis. J Fish Biol 60:1358–1362CrossRefGoogle Scholar
  23. Qin CG, Huang KX, Xu HB (2002) Protective effect of polysaccharides from the loach on the in vitro and in vivo peroxidative damage of hepatocyte. J Nutr Biochem 13:592–597PubMedCrossRefGoogle Scholar
  24. Santos MA, Pacheco M (1996) Anguilla anguilla L. Stress biomarkers recovery in clean water and secondary treated pulp mill effluent. Ecotoxicol Environ Saf 35:96–100PubMedCrossRefGoogle Scholar
  25. Shen XM, Zhang HY, Hua R (1991) Effect of environmental factors on haematological characters of blunt-snout bream (Megalobrama amblycephala YIH). Acta Ecol Sin 11:92–94Google Scholar
  26. Skjervold PO, Fjaera SO, Ostby PB, Einen O (2001) Live-chilling and crowding stress before slaughter of Atlantic salmon (Salmo salar). Aquaculture 192:265–280CrossRefGoogle Scholar
  27. Svoboda M, Kouřil J, Hamáčková J, Kaláb P, Savina L, Svobodová Z, Vykusová B (2001) Biochemical profile of blood plasma of tench (Tinca tinca L.) during pre- and postspawning period. Acta Vet Brno 70:259–268Google Scholar
  28. Svobodova Z, Vykusova B, Modra H, Jarkovsky J, Smutna M (2006) Haematological and biochemical profile of harvest-size carp during harvest and post-harvest storage. Aquacult Res 37:959–965CrossRefGoogle Scholar
  29. Tavares-Dias M, Moraes FR (2007) Haematological and biochemical reference intervals for farmed channel catfish. J Fish Biol 71:383–388CrossRefGoogle Scholar
  30. Torres P, Tort L, DePauw N, Joyce J (1991) Effects of stress and metal exposure on blood parameters and liver metabolism in rainbow trout. Spec Publ Eur Aquacult Soc 14:312–313Google Scholar
  31. Walsh PJ, Mayer GD, Medina M, Bernstein ML, Barimo JF, Mommsen TP (2003) A second glutamine synthetase gene with expression in the gills of the Gulf toadfish (Opsanus beta). J Exp Biol 206:1523–1533PubMedCrossRefGoogle Scholar
  32. Wang YJ, Lu WM, Hu MH, Wang WM, Song LM, Lv BP, Zhang SY (2007) Feeding and growth of Misgurnus anguillicaudatus during early development under artificial rearing conditions. J Huazhong Agric Univ 26:665–669Google Scholar
  33. Wood ME, Yasutate WT, Halver JE, Woodall AN (1960) Chemical and histological studies of wild and hatchery salmon in fresh water. Trans Am Fish Soc 89:301–307CrossRefGoogle Scholar
  34. Xu PC, Cao CH (1989) On hematology of the blood of fishes cultured in the lake pen. J Fish China 13:346–352Google Scholar
  35. Xu WY (2004) Relationship between haematological indices and sexuality in Misgurnus anguillicaudatus. Fish Sci 23:15–17Google Scholar
  36. Yin J, Zhao ZS, Chen XQ, Li YQ, Zhu LY (2005) Karyotype comparison of diploid and tetraploid loach, Misgurnus anguillicanudatus. Acta Hydrobiol Sinica 29:469–472Google Scholar
  37. Yu XJ, Zhou T, Li YC, Li K, Zhou M (1989) Chromosomes of Chinese freshwater fishes. Science Press, BeijingGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Xiaoyun Zhou
    • 1
  • Mingyun Li
    • 2
  • Khalid Abbas
    • 1
  • Weimin Wang
    • 1
  1. 1.College of Fishery, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of EducationHuazhong Agricultural UniversityWuhan, HubeiPeoples Republic of China
  2. 2.Faculty of Life Sciences and BiotechnologyNingbo UniversityNingboPeoples Republic of China

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