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The growth hormone-encoding gene isolated and characterized from Labeo rohita Hamilton is expressed in CHO cells under the control of constitutive promoters in ‘autotransgene’ constructs

  • R. Rajesh
  • K. C. MajumdarEmail author
Article

Abstract

The growth hormone (GH) gene along with its regulatory sequences has been isolated from the blood and pituitary gland of Labeo rohita. This GH gene is approximately 2.8 kb long and consists of five exons and four introns of varying sizes with AG/TA in its exon–intron junctions. The promoter has a single cyclic AMP response unit (CRE) element, TATA, CAT and several Pit 1 binding sequences. The 1169-bp gene transcript starts 54 bp upstream of the ATG initiation codon and has two polyadenylation signals, ATTAAA, after the TAG stop codon. The mature mRNA has the poly (A) tail inserted 16 bp downstream of the second polyadenylation signal. Four chimeric ‘autotransgenes’ were constructed having either histone 3 or β-actin promoter and cDNA or the total GH gene. The functionality of the individual components of the autotransgene was determined in the Chinese hamster ovary (CHO) cells by transfection experiments. Based on the results, the transcription of the GH gene is initiated at the transcription start signal of the respective promoters and terminates at the 3′ regulatory sequence of the GH gene. Expression of GH in CHO cells shows that the fish promoters are active, the splicing signal is recognized, and the mRNA produced is stable and translated. The GH protein produced is effectively translocated and secreted into the medium. These results indicate the usefulness of CHO cells in determining the property of individual components of autotransgenes constructed from L. rohita and overall functional commonality between fish and mammal.

Keywords

Autotransgene CHO cells Growth hormone gene Heterologous expression Labeo rohita 

Notes

Acknowledgements

We are indebted to Dr. L. Singh, Director, CCMB for his constant support; Dr. S.K. Rath for the initial experiments on library construction and screening; Dr. B. Venkatesh, Institute of Molecular Cell Biology, Singapore for providing GH cDNA of goldfish; Dr. Shashi Singh and Mr. Parthasarathy for CHO cell transfection; Dr. K. Uma Devi for correcting; Mr. M. Ranga Rao for typing the manuscript. Thanks are due to two anonymous reviewers for their suggestions. Financial support by the University Grants Commission and the Council of Scientific and Industrial Research, Government of India for a fellowship to RR and the Department of Biotechnology, Government of India for a grant (BT/AA/03/70(A)/94) to KCM is gratefully acknowledged.

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© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Centre for Cellular and Molecular BiologyHyderabadIndia

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