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Fish Physiology and Biochemistry

, Volume 34, Issue 2, pp 151–158 | Cite as

Anti-oxidant status in embryonic, post-hatch and larval stages of Asian seabass (Lates calcarifer)

  • N. KalaimaniEmail author
  • N. Chakravarthy
  • R. Shanmugham
  • A. R. Thirunavukkarasu
  • S. V. Alavandi
  • T. C. Santiago
Article

Abstract

The concentrations of anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and selenium-dependent glutathione peroxidase (SeGPx), and low molecular weight free-radical scavengers such as reduced glutathione (GSH) and ascorbic acid (vitamin C) were evaluated during the period from gastrulation (GS) to 25 days post-hatch (dph) in the larvae of Asian Seabass, Lates calcarifer. Oxidative damage due to lipid peroxidation (LPO) was also assessed, by evaluation of the formation of malondialdehyde (MDA). All the three anti-oxidant enzymes, SOD, CAT and GPx, showed high activities during gastrulation, suggesting an increased metabolic rate during the period of embryonic development. Though the SOD activity apparently decreased progressively during 3–20 dph of larval development, the difference was not significant. CAT showed high activity during gastrulation and remained constant up to 3 dph, suggesting an increased need to metabolise hydrogen peroxide (H2O2) and organic peroxides. In contrast, SeGPx activity increased progressively from 5 dph to 25 dph during larval development, indicating an increased need to detoxify lipid peroxides. This is evident from the observation of increased lipid peroxidation from 10 dph to 25 dph during larval development. GSH levels were low at gastrulation, indicating increased metabolic rate and formation of lipid radicals during this period, corresponding to the decrease in the level of ascorbic acid, which is consumed for regeneration of GSH.

Keywords

Anti-oxidant enzymes Ascorbic acid Eggs Embryos Gastrulation Larvae Lates calcarifer Malondialdehyde Reactive oxygen species 

Notes

Acknowledgements

The authors are grateful to A.G. Ponniah, Director, CIBA, for critically going through the manuscript and for his valuable suggestions. The help rendered by R. Subburaj, Technical Officer, R. Thiagarajan, Technical Assistant and V. Anuradha, Senior Research Fellow, is gratefully acknowledged.

References

  1. Aceto A, Amicarelli F, Sacchetta P, Dragan B, Bucciarelli T, Masciocco L, Miranda M, Dillio C (1994) Developmental aspects of detoxifying enzymes in fish (Salmo iridaeus). Free Radic Res 21:285–294PubMedCrossRefGoogle Scholar
  2. Arun S, Subramanian P (1998) Antioxidant enzymes in fresh water prawn Macrobrachium malcolmsonii during embryonic and larval development. Comp Biochem Physiol B 121:273–277CrossRefGoogle Scholar
  3. Beutler E, Kelley BM (1963) The effect of sodium nitrate on red cell glutathione. Experientia 19:96–97PubMedCrossRefGoogle Scholar
  4. Chamiec T, Herbaezynskacedro K, Ceremuzynski L (1996) Effects of antioxidant vitamin C and E on signal-over aged electrocardiogram in acute myocardial infarction. Am J Cardiol 77:237–241PubMedCrossRefGoogle Scholar
  5. Comporti M (1987) Glutathione depleting agents and lipid peroxidation. Chem Phys Lipids 45:143–169PubMedCrossRefGoogle Scholar
  6. Danchenko OO, Kalytka VV (2002) Formation of antioxidant defence system of geese in embryogenesis and early postnatal ontogenesis. Ukr Biokhim Zh 74:120–124PubMedGoogle Scholar
  7. Dandapat J, Chainy GBN, Rao KJ (2003) Lipid peroxidation and antioxidant defence status during larval development and metamorphosis of giant prawn, Macrobrachium rosenbergii. Comp Biochem Physiol C 135:221–233Google Scholar
  8. Devasagayam TPA (1986) Lipid peroxidation in rat uterus. Biochem Biophys Acta 876:507–514PubMedGoogle Scholar
  9. Fernandez-Diaz C, Yufera M, Canavate JP, Moyano PJ, Alarcon FJ, Diaz M (2001) Growth and physiological changes during metamorphosis of Senegal Sole reared in the laboratory. J Fish Biol 58:1086–1097CrossRefGoogle Scholar
  10. Fernandez-Diaz C, Kopecka I, Canavate JP, Sarasquete C, Sole M (2006) Variations on development and stress defences in Solea senegalensis larvae fed on live and microencapsulated diets. Aquaculture 251:573–584CrossRefGoogle Scholar
  11. Fridovich I (2004) Mitochondria: are they the seat of senescence? Aging Cell 3:13–16PubMedCrossRefGoogle Scholar
  12. Godin DV, Nichols CR, Hoekstra KA, Garnett ME, Cheng KM (2001) Red cell and plasma antioxidant components and atherosclerosis in Japanese quail: a time-course study. Res Commun Mol Pathol Pharmacol 110:27–51PubMedGoogle Scholar
  13. Halliwell B, Gutteridge JMC (1989) Protection against Oxy-rad in biological systems: the superoxide theory of oxygen toxicity. In: Halliwel B, Gutteridge JMC (eds) Free radicals in biology and medicine. Clarendon Press, OxfordGoogle Scholar
  14. Jialal I, Devaraj S, Kaul N (2001) The effect of alpha-tocopherol on monocyte proatherogenic activity. J Nutr 131:3893–3943Google Scholar
  15. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  16. Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the auto oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474PubMedCrossRefGoogle Scholar
  17. McCay PB (1985) Vitamin E: interactions with free radicals and ascorbate. Annu Rev Nutr 5:323–340PubMedCrossRefGoogle Scholar
  18. McLennan SV, Heffeman S, Wright L, Rae C, Fisher E, Yue DK, Turtle JR (1991) Changes in hepatic glutathione metabolism in diabetes. Diabetes 40:340–344CrossRefGoogle Scholar
  19. Miller JK, Brzezinska-Slebodzinska E, Madsen FC (1993) Oxidative stress, antioxidants and animal function. J Dairy Sci 76:2812–2821PubMedCrossRefGoogle Scholar
  20. Mourente G, Tocher DR, Diaz E, Grau A, Pastor E (1999) Relationship between antioxidants, antioxidant enzyme activities and lipid peroxidation products during early development in Dentax dentax eggs and larvae. Aquaculture 179:309–324CrossRefGoogle Scholar
  21. Ogino T, Kawabata T, Awadi M (1989) Stimulation of glutathione synthesis in iron loaded mice. Biochem Biophys Acta 1006:131–135PubMedGoogle Scholar
  22. Omaye S, Turnbull JD, Sauberlich HE (1979) Selected methods for the determination of ascorbic acid in animal cell, tissues and fluids. Methods Enzymol 62:1–11Google Scholar
  23. Oup P, Nourooz-Zadeh J, Tritschler HJ, Wolff S (1996) Activation of aldose reductase in rat lens and metal–ion chelation by aldose reductase inhibitors and lipoic acid. Free Radic Res 25:337–346CrossRefGoogle Scholar
  24. Peters LD, Livingstone DR (1996) Antioxidant enzyme activities in embryologic and early larval stages of turbot. J Fish Biol 49:986–997CrossRefGoogle Scholar
  25. Peters LD, Porte C, Livingstone DR (2001) Variation of antioxidant enzyme activities in sprat (Sprattus sprattus) larvae in mixed zooplankton from the southern North Sea. Mar Pollut Bull 42:1087–1095PubMedCrossRefGoogle Scholar
  26. Rotruck JT, Poe AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra NG (1973) Selenium: biochemical role as a component of glutathione peroxidase purification and assay. Science 179:588–590PubMedCrossRefGoogle Scholar
  27. Rudneva II (1999) Antioxidant system of Black Sea animals in early development. Comp Biochem Physiol C 122:265–271PubMedGoogle Scholar
  28. Shen X, Aw TY, Jones DP (1990) Glutathione dependent protection against oxidative injury. Pharmacol Ther 47:61–71CrossRefGoogle Scholar
  29. Sinha SK (1972) Colorimetric assay of catalase. Ann Biochem 47:389–395CrossRefGoogle Scholar
  30. Sole M, Potrykus J, Fernandez-Diaz C, Blasco J (2004) Variations on stress defences and metallothionein levels in the Senegal sole, Solea senegalenisis, during early larval stages. Fish Physiol Biochem 30:57–66CrossRefGoogle Scholar
  31. Starrs AP, Orgeig S, Daniels CB, Davies M, Lopatko OV (2001) Antioxidant enzymes in the developing lungs of egg-laying and metamorphosing vertebrates. J Exp Biol 204:3973–3981PubMedGoogle Scholar
  32. Story KB (1996) Oxidative stress: animal adaptations in nature. Braz J Med Biol Res 29:1715–1733Google Scholar
  33. Wilhelm Filho D (1996) Fish antioxidant defenses—a comparative approach. Braz J Med Biol Res 29:1735–1742PubMedGoogle Scholar
  34. Yufera M, Parra G, Santiago R, Carrascosa M (1999) Growth, carbon, nitrogen and caloric content of Solea snegalensis (Pisces: Soleidae) from egg fertilization to metamorphosis. Mar Biol 134:43–49CrossRefGoogle Scholar
  35. Zielinski S, Portner HO (2000) Oxidative stress and antioxidative defense in cephalopods: a function of metabolic rate or age? Comp Biochem Physiol B Biochem Mol Biol 125:147–160PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • N. Kalaimani
    • 1
    Email author
  • N. Chakravarthy
    • 1
  • R. Shanmugham
    • 1
  • A. R. Thirunavukkarasu
    • 1
  • S. V. Alavandi
    • 1
  • T. C. Santiago
    • 1
  1. 1.Central Institute of Brackishwater AquacultureChennaiIndia

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