Fish Physiology and Biochemistry

, Volume 34, Issue 2, pp 151–158 | Cite as

Anti-oxidant status in embryonic, post-hatch and larval stages of Asian seabass (Lates calcarifer)

  • N. KalaimaniEmail author
  • N. Chakravarthy
  • R. Shanmugham
  • A. R. Thirunavukkarasu
  • S. V. Alavandi
  • T. C. Santiago


The concentrations of anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and selenium-dependent glutathione peroxidase (SeGPx), and low molecular weight free-radical scavengers such as reduced glutathione (GSH) and ascorbic acid (vitamin C) were evaluated during the period from gastrulation (GS) to 25 days post-hatch (dph) in the larvae of Asian Seabass, Lates calcarifer. Oxidative damage due to lipid peroxidation (LPO) was also assessed, by evaluation of the formation of malondialdehyde (MDA). All the three anti-oxidant enzymes, SOD, CAT and GPx, showed high activities during gastrulation, suggesting an increased metabolic rate during the period of embryonic development. Though the SOD activity apparently decreased progressively during 3–20 dph of larval development, the difference was not significant. CAT showed high activity during gastrulation and remained constant up to 3 dph, suggesting an increased need to metabolise hydrogen peroxide (H2O2) and organic peroxides. In contrast, SeGPx activity increased progressively from 5 dph to 25 dph during larval development, indicating an increased need to detoxify lipid peroxides. This is evident from the observation of increased lipid peroxidation from 10 dph to 25 dph during larval development. GSH levels were low at gastrulation, indicating increased metabolic rate and formation of lipid radicals during this period, corresponding to the decrease in the level of ascorbic acid, which is consumed for regeneration of GSH.


Anti-oxidant enzymes Ascorbic acid Eggs Embryos Gastrulation Larvae Lates calcarifer Malondialdehyde Reactive oxygen species 



The authors are grateful to A.G. Ponniah, Director, CIBA, for critically going through the manuscript and for his valuable suggestions. The help rendered by R. Subburaj, Technical Officer, R. Thiagarajan, Technical Assistant and V. Anuradha, Senior Research Fellow, is gratefully acknowledged.


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • N. Kalaimani
    • 1
    Email author
  • N. Chakravarthy
    • 1
  • R. Shanmugham
    • 1
  • A. R. Thirunavukkarasu
    • 1
  • S. V. Alavandi
    • 1
  • T. C. Santiago
    • 1
  1. 1.Central Institute of Brackishwater AquacultureChennaiIndia

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