Fish Physiology and Biochemistry

, Volume 33, Issue 2, pp 167–172

Effects of dietary thiamin on the physiological status of the grouper Epinephelus coioides

  • J. W. Huang
  • L. X. Tian
  • Z. Y. Du
  • H. J. Yang
  • Y. J. Liu


This study was conducted to evaluate the effects of dietary thiamin on the physiological status of the juvenile grouper, Epinephelus coioides. Graded levels of thiamin (0.08, 0.50, 2.12, 3.15, 4.63, 12.37 mg thiamin kg−1 diet) were fed to grouper juveniles (mean weight: 16.97 ± 0.14 g) for 10 weeks. Although fish fed the thiamin-deficient (TD) diet showed no obvious symptoms of thiamin deficiency or increased mortality, those fed the lowest doses of thiamin (0.08 and 0.50 mg thiamin kg−1 diet) had significantly decreased transketolase activity in the liver. In addition, the level of liver thiobarbituric acid reactive substances in fish fed the TD diet was 33–67% higher than that in fish with the thiamin-supplemented diet. There were no significant differences in superoxide dismutase activity between the different groups of fish.


Epinephelus coioides Grouper Superoxide dismutase (SOD) Thiamin Thiobarbituric acid reactive substances (TBARs) Transketolase 


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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • J. W. Huang
    • 1
  • L. X. Tian
    • 1
  • Z. Y. Du
    • 1
  • H. J. Yang
    • 1
  • Y. J. Liu
    • 1
  1. 1.Institute of Aquatic Economic Animals, School of Life SciencesSun Yat-sen UniversityGuangzhouP.R. China

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