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Fish Physiology and Biochemistry

, Volume 33, Issue 2, pp 93–101 | Cite as

Administration of a herbal immunoregulation mixture enhances some immune parameters in carp (Cyprinus carpio)

  • Chuntao Yuan
  • Dongmei Li
  • Wei Chen
  • Fangfang Sun
  • Guanghong Wu
  • Yi Gong
  • Jianqing Tang
  • Meifang Shen
  • Xiaodong HanEmail author
Original Paper

Abstract

The herbal immunoregulation mixture (HIRM) were extracts of several traditional Chinese medicines (TCMs): Astragalus membranaceus (from the root and stem), Polygonum multiflorum Thunb. (from the root), Isatis tinctoria L. (from the root), Glycyrrhiza glabra (from the stem). Immune parameters, which included macrophage phagocytic activity, macrophage reactive oxygen species (ROS), activity of serum lysozyme, nitric oxide synthetase (NOS) and superoxide dismutase (SOD), levels of total serum protein, globulin, albumin, triglyceride and cholesterol, were determined in carp that had been fed diets containing HIRM at 0.5% or 1% for 30 days. The results showed that, compared with those in the control group, the diets with 0.5% and 1% HIRM resulted in significant increase in macrophage phagocytic activity, macrophage ROS and the levels of total protein, globulin, albumin and NOS activity in serum (P < 0.05), and no significant difference was found in SOD, lysozyme activities and triglyceride level (P > 0.05). On the other hand, 0.5% HIRM led to evident enhancement of NOS activity and cholesterol level compared to 1% HIRM. These results indicated that HIRM might elevate the function of immunity in carp (Cyprinus carpio).

Keywords

Biochemical indices of serum Cyprinus carpio HIRM Lysozyme Macrophage phagocytic activity NOS ROS SOD 

Notes

Acknowledgments

The research was supported by a grant from the Natural Science Foundation of Jiangsu Province (BK2005103) and Fishery Engineering Program of Jiangsu Province (PJ2005-51). We thank Yong Wang and Jun Cao for their technical assistance in the experiment.

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Chuntao Yuan
    • 1
  • Dongmei Li
    • 1
  • Wei Chen
    • 1
  • Fangfang Sun
    • 1
  • Guanghong Wu
    • 2
  • Yi Gong
    • 1
  • Jianqing Tang
    • 2
  • Meifang Shen
    • 2
  • Xiaodong Han
    • 1
    Email author
  1. 1.Immunology and Reproductive Biology LaboratoryMedical School, Nanjing UniversityNanjingP. R. China
  2. 2.Freshwater Fisheries Research InstituteNanjingP. R. China

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