Familial Cancer

, Volume 8, Issue 1, pp 75–83 | Cite as

Major contribution from recurrent alterations and MSH6 mutations in the Danish Lynch syndrome population

  • Mef Nilbert
  • Friedrik P. Wikman
  • Thomas V. O. Hansen
  • Henrik B. Krarup
  • Torben F. Örntoft
  • Finn C. Nielsen
  • Lone Sunde
  • Anne-Marie Gerdes
  • Dorthe Cruger
  • Susanne Timshel
  • Marie-Louise Bisgaard
  • Inge Bernstein
  • Henrik Okkels


An increasing number of mismatch-repair (MMR) gene mutations have been identified in hereditary nonpolyposis colorectal cancer (HNPCC) or Lynch syndrome. This study presents the population-based Danish MMR gene mutation profile, which contains 138 different MMR gene alterations. Among these, 88 mutations in 164 families are considered pathogenic and an additional 50 variants from 76 families are considered to represent variants of unknown pathogenicity. The different MMR genes contribute to 40% (MSH2), 29% (MLH1), and 22% (MSH6) of the mutations and the Danish population thus shows a considerably higher frequency of MSH6 mutations than previously described. Although 69/88 (78%) pathogenic mutations were present in a single family, previously recognized recurrent/founder mutations were causative in 75/137 (55%) MLH1/MSH2 mutant families. In addition, the Danish MLH1 founder mutation c.1667+2_1667_+8TAAATCAdelinsATTT was identified in 14/58 (24%) MLH1 mutant families. The Danish Lynch syndrome population thus demonstrates that MSH6 mutations and recurrent/founder mutations have a larger contribution than previously recognized, which implies that the MSH6 gene should be included in routine diagnostics and suggests that directed analysis of recurrent/founder mutations may be feasible e.g. in families were diagnostic material is restricted to archival tissue.


MLH1 MSH2 MSH6 Hereditary nonpolyposis colorectal cancer HNPCC 



Financial support was granted from the Danish Cancer Society and from the Hvidovre University Hospital. We would also like to acknowledge surgeons, pathologists, and geneticists for identifying these families and reporting data to the national HNPCC-register.


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mef Nilbert
    • 1
  • Friedrik P. Wikman
    • 2
  • Thomas V. O. Hansen
    • 3
  • Henrik B. Krarup
    • 4
  • Torben F. Örntoft
    • 2
  • Finn C. Nielsen
    • 3
  • Lone Sunde
    • 5
  • Anne-Marie Gerdes
    • 6
  • Dorthe Cruger
    • 7
  • Susanne Timshel
    • 8
  • Marie-Louise Bisgaard
    • 9
  • Inge Bernstein
    • 8
  • Henrik Okkels
    • 4
  1. 1.Clinical Research Centre and HNPCC-RegisterCopenhagen University, Hvidovre University HospitalHvidovreDenmark
  2. 2.Department of Clinical BiochemistryAarhus University HospitalAarhusDenmark
  3. 3.Department of Clinical BiochemistryRigshospitalet, Copenhagen University HospitalCopenhagenDenmark
  4. 4.Department of Clinical BiochemistryAalborg University HospitalAalborgDenmark
  5. 5.Department of Clinical GeneticsAarhus University HospitalAarhusDenmark
  6. 6.Department of Clinical GeneticsOdense University HospitalOdenseDenmark
  7. 7.Department of Clinical GeneticsVejle HospitalVejleDenmark
  8. 8.HNPCC-RegisterHvidovre University HospitalHvidovreDenmark
  9. 9.Department of Cellular and Molecular Medicine, Panum InstituteCopenhagen UniversityCopenhagenDenmark

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