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Evolutionary Ecology

, Volume 30, Issue 4, pp 663–684 | Cite as

Geographic structuring into vicariant species-pairs in a wide-ranging, high-dispersal plant–insect mutualism: the case of Ficus racemosa and its pollinating wasps

  • A. Bain
  • R. M. Borges
  • M. H. Chevallier
  • H. Vignes
  • N. Kobmoo
  • Y. Q. Peng
  • A. Cruaud
  • J. Y. Rasplus
  • F. Kjellberg
  • M. Hossaert-Mckey
Original Paper

Abstract

Ficus and their mutualistic pollinating wasps provide a unique model to investigate joint diversification in a high dispersal system. We investigate genetic structuring in an extremely wide-ranging Ficus species, Ficus racemosa, and its pollinating wasp throughout their range, which extends from India to Australia. Our samples were structured into four large, vicariant populations of figs and wasps which may correspond to distinct (sub)species, located in India, China-Thailand, Borneo, and Australia. However, the genetically most divergent group was the Indian population for the figs and the China-Thailand population for the wasps, suggesting different evolutionary histories of populations. Molecular dating for the wasps shows that diversification of the pollinator clade is surprisingly old, beginning about 13.6 Ma. Data on both the host fig species and its pollinating wasps suggest that strong genetic flow within biogeographic groups over several hundreds of kilometers has limited genetic and morphological differentiation and, potentially, local adaptation. This is probably due to long-distance dispersal of pollinating wasps. The genetic clustering into large geographic units observed in F. racemosa and its pollinators is reminiscent of what can be observed in some other high-dispersal organisms characterized by morphology that varies little over huge distances. The implications of strong gene flow for diversification processes and adaptation to different ecological conditions in Ficus and their pollinating wasps are just beginning to emerge.

Keywords

Agaonidae Genetic structuring Ficus Phylogeography 

Notes

Acknowledgments

This study was funded by the by the ANR Projects “nicefigs” (ANR-09-BLAN-0392-CSD 7; NSC 99-2923-B-002-001-MY3) and “biofigs” (ANR BDIV-006-001)/National Science Council, Taiwan, R. O. C. Led by M.H.M. We thank Marc Ancrenaz, James Cook, and Edmond Dounias for their help in collecting samples, and Doyle McKey for his useful comments on our paper. We are also grateful to the staff of the Center for Ecological Sciences, Bangalore and of Xishuangbanna Tropical Botanical Garden (XTBG, China) for their field assistance. Laboratory manipulations were done at CEFE (SMGE), CBGP and at the LABEX CEMEB facilities.

Supplementary material

10682_2016_9836_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 18 kb)
10682_2016_9836_MOESM2_ESM.docx (300 kb)
Supplementary material 2 (DOCX 299 kb)

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • A. Bain
    • 1
    • 2
  • R. M. Borges
    • 3
  • M. H. Chevallier
    • 1
    • 4
  • H. Vignes
    • 1
    • 5
  • N. Kobmoo
    • 1
    • 6
  • Y. Q. Peng
    • 7
  • A. Cruaud
    • 8
  • J. Y. Rasplus
    • 8
  • F. Kjellberg
    • 1
  • M. Hossaert-Mckey
    • 1
  1. 1.CEFE UMR 5175, CNRSUniversité de Montpellier, Université Paul-Valéry Montpellier – EPHEMontpellier, Cedex 5France
  2. 2.Institute of Ecology and Evolutionary Biology, College of Life ScienceNational Taiwan UniversityTaipeiTaiwan
  3. 3.Indian Institute of ScienceCentre for Ecological SciencesBangaloreIndia
  4. 4.CIRAD, Station de Ligne-Paradi, Pôle de Protection des Plantes - 3PsSaint-PierreFrance
  5. 5.CIRAD UMR AGAPMontpellierFrance
  6. 6.National Center for Genetic Engineering and Biotechnology (BIOTEC)Khlong LuangThailand
  7. 7.Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunmingChina
  8. 8.INRA - UMR 1062 CBGP (INRA/IRD/CIRAD/supagro)Montferrier Sur LezFrance

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