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Rapid identification of yellow-flowered gene Bofc in cauliflower (Brassica oleracea var. botrytis) by bulked segregant analysis and whole-genome resequencing

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Abstract

The accumulation of carotenoids is related to pigment formation in flowers and other organs. In order to elucidate the genetic variation of flower color in cauliflower, a F2 segregating population was obtained from yellow-flowered cauliflower ‘QGS’ and white-flowered Chinese kale ‘JYJ’. Bulked segregant analysis combined with the whole-genome resequencing was employed to locate the target region for Flower Color. In this study, Bofc was anchored on chromosome C03 with a single peak, and further mapped to 164 kb interval with 0.28 cM genetic distance. BoCCD4, encoding carotenoid cleavage dioxygenase 4 in the carotenoid metabolic pathway, was selected as the most likely candidate gene. Sequence analysis revealed that an insertion of a 10,608 bp CACTA-like transposon in the coding region led to the loss-of-function of the BoCCD4 gene in cauliflower. These results will provide further understanding of the genetic mechanism controlling flower color in Brassica oleracea species.

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Acknowledgements

We thank Prof. Deling Sun and Dr. Xiaoli Zhang (Tianjin Academy of Agricultural Sciences) for kindly providing the cauliflower reference genome ‘C-8’ and Dr. Bao Zhang (Huazhong Agricultural University) for kind suggestions about the variations of BoCCD4. This work was supported by the Modern Agro-Industry Technology Research System, China (CARS-23-B-09), Youth Science Fund of Hubei Academy of Agricultural Sciences (2020NKYJJ04) and the National Natural Science Foundation of China (31902058).

Author information

CY performed the major experiments. YH, ZL, ZJ, WY, and FZ contributed to planting materials and detecting the phenotypes. CY wrote and revised the manuscript with help from ZL, ZQ, and FG. All authors reviewed this manuscript.

Correspondence to Zhengming Qiu.

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Yan, C., Huang, Y., Liu, Z. et al. Rapid identification of yellow-flowered gene Bofc in cauliflower (Brassica oleracea var. botrytis) by bulked segregant analysis and whole-genome resequencing. Euphytica 216, 26 (2020). https://doi.org/10.1007/s10681-020-2560-9

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Keywords

  • Cauliflower
  • Flower color
  • Map-based cloning
  • Carotenoid
  • Carotenoid cleavage dioxygenase 4