The accumulation of carotenoids is related to pigment formation in flowers and other organs. In order to elucidate the genetic variation of flower color in cauliflower, a F2 segregating population was obtained from yellow-flowered cauliflower ‘QGS’ and white-flowered Chinese kale ‘JYJ’. Bulked segregant analysis combined with the whole-genome resequencing was employed to locate the target region for Flower Color. In this study, Bofc was anchored on chromosome C03 with a single peak, and further mapped to 164 kb interval with 0.28 cM genetic distance. BoCCD4, encoding carotenoid cleavage dioxygenase 4 in the carotenoid metabolic pathway, was selected as the most likely candidate gene. Sequence analysis revealed that an insertion of a 10,608 bp CACTA-like transposon in the coding region led to the loss-of-function of the BoCCD4 gene in cauliflower. These results will provide further understanding of the genetic mechanism controlling flower color in Brassica oleracea species.
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We thank Prof. Deling Sun and Dr. Xiaoli Zhang (Tianjin Academy of Agricultural Sciences) for kindly providing the cauliflower reference genome ‘C-8’ and Dr. Bao Zhang (Huazhong Agricultural University) for kind suggestions about the variations of BoCCD4. This work was supported by the Modern Agro-Industry Technology Research System, China (CARS-23-B-09), Youth Science Fund of Hubei Academy of Agricultural Sciences (2020NKYJJ04) and the National Natural Science Foundation of China (31902058).
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Yan, C., Huang, Y., Liu, Z. et al. Rapid identification of yellow-flowered gene Bofc in cauliflower (Brassica oleracea var. botrytis) by bulked segregant analysis and whole-genome resequencing. Euphytica 216, 26 (2020). https://doi.org/10.1007/s10681-020-2560-9
- Flower color
- Map-based cloning
- Carotenoid cleavage dioxygenase 4