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Mothers’ command: phenotypes changes resulting from reciprocal interploidy crosses

Abstract

Polyploidy is considered a valuable tool in plant breeding because can increase the size of flowers, leaves, and fruits or generate seedless plants, such as infertile triploids. Triploid plants can be obtained by reciprocal interploidy crosses (4x × 2x or 2x × 4x), but the influence of parent-of-origin effects in the plants’ phenotype caused by these interploidy crosses, as well as the interactions between cytoplasm and nucleus in these crosses are not clearly defined. Therefore, this work aimed to characterize the morphology and physiology of triploid tomato plants leaves by parent-of-origin 2x and 4x. For this purpose, we induct the tetraploidy with colchicine from diploid plants to performed the reciprocal interploidy crosses and obtained the triploid hybrids by differents parents-of-origin (2x or 4x). Histological slides from the leaves of all plant genotypes were assembled to analyze the anatomy, morphology and to measure the chlorophyll a, b, and the total index. Tetraploid tomato plants showed greater adaxial and total epidermis leaf thickness. Triploid plants by parent-of-origin 4x presented higher leaf thickness and spongy parenchyma than the triploid plants by parent-of-origin 2x, as well as higher rates of chlorophyll a and b, and an inbalance in the ratio of chlorophyll index a/b. We conclude that changes in cytoplasmatic organelles, caused by tetraploidization, can be transferred from parent-of-origin 4x to triploid plants. However, further studies are necessary, aiming to understand the genetic interaction between the cytoplasmatic genome (and consequently organelles) and the nucleus genome of triploid plants by differents parents-of-origin.

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Acknowledgements

We thank CCSM—Centro de Citricultura Sylvio Moreira (IAC-Cordeirópolis-SP/Brazil), CEIS—Centro de Estudos de Insetos Sociais (UNESP–Rio Claro-SP/Brazil) and Laboratório de Fisiologia Vegetal e Cultura de Tecidos (UFSCar campus Araras-SP/Brazil) for the laboratory infrastructure, the Sakata Seeds Sudamérica Company for providing the seeds and the greenhouses, and Carolina Grando for the first english review. We also thank Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPQ for the scholarship.

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Correspondence to Luciano Delmondes de Alencar.

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de Alencar, L.D., Azevedo, P. & Latado, R.R. Mothers’ command: phenotypes changes resulting from reciprocal interploidy crosses. Euphytica 216, 21 (2020). https://doi.org/10.1007/s10681-020-2557-4

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Keywords

  • Tomato
  • Parent-of-origin effects
  • Triploidy
  • Foliar anatomy
  • Chlorophyll index