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Euphytica

, 215:205 | Cite as

Identification of QTLs related to cadmium tolerance from wild rice (Oryza nivara) using a high-density genetic map for a set of introgression lines

  • Xin Ma
  • Xiaoping Chen
  • Jie Zhao
  • Shanshan Wang
  • Lubin Tan
  • Chuanqing Sun
  • Fengxia LiuEmail author
Article
  • 65 Downloads

Abstract

Cadmium (Cd) is a toxic heavy metal that is harmful to human health. Oryza nivara, a wild rice species, serves as a germplasm pool for various favorable genes that can be used in cultivated rice. To detect quantitative trait loci (QTLs) and germplasm related to Cd tolerance in O. nivara, we analyzed one set of introgression lines with a high-density genetic map. Seven QTLs related to Cd tolerance were identified on chromosomes 2, 4, 6, and 8, and an allele of one locus (Bin774) from O. nivara affected two phenotypic traits and was able to increase Cd tolerance in rice. In a further analysis of GeneChip data released by the National Center for Biotechnology Information, we explored five candidate genes induced by Cd stress in the QTL region: two oxidoreductase genes (aldo-keto reductase genes LOC_Os04g27060 and LOC_Os04g26920); one terpene synthase gene (LOC_Os04g27190); one cysteine-rich receptor-like protein kinase gene (LOC_Os04g25060); and one serine carboxypeptidase homolog gene OsSCP23 (LOC_Os04g25560). One Cd-tolerant introgression line (Ra44) was also obtained, with a significantly higher survival rate under Cd stress (93.8%) compared to recipient parent 93-11 (2.3%). Identification of candidate genes and the Cd-tolerant introgression line Ra44 should facilitate further investigation of the mechanism of rice tolerance to Cd contamination.

Keywords

Nivara wild rice Cadmium tolerance QTL Introgression line 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31671647).

Supplementary material

10681_2019_2522_MOESM1_ESM.xlsx (18 kb)
Supplementary material 1 (XLSX 18 kb)
10681_2019_2522_MOESM2_ESM.xlsx (11 kb)
Supplementary material 2 (XLSX 10 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.National Center for Evaluation of Agricultural Wild Plants (Rice), MOE Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and BreedingChina Agricultural UniversityBeijingChina

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