, 215:179 | Cite as

Marker-assisted pyramiding of major blast resistance genes in Swarna-Sub1, an elite rice variety (Oryza sativa L.)

  • Parashuram Patroti
  • B. Vishalakshi
  • B. Umakanth
  • J. Suresh
  • P. Senguttuvel
  • M. Sheshu MadhavEmail author


Swarna-Sub1, a ruling submergence tolerant variety of rice, showed a significant decrease in the yield in major blast disease prone areas, which is caused by fungus Magnaporthe oryzae (Pyricularia oryzae). To overcome this problem, three major blast resistance genes viz., Pi1, Pi2, and Pi54 were pyramided through marker-assisted backcross breeding using donors, Swarna-LT (having Pi1 + Pi54) and Swarna-A51 (having Pi2). Foreground selection was carried out using molecular markers tightly linked to three blast resistance genes and also submergence tolerance for retaining the Sub1 gene in the recurrent parent. Based on disease resistance and agro-morphological performance of backcross-derived lines, we selected the best 25 plants of two- and three-gene pyramided BC3F2 homozygous lines with a maximum of ~ 93.5% recurrent parent genome and were further advanced to the BC3F5 generation. The stringent recurrent parent genome recovery analysis using SSR markers limited the linkage drag from a minimum of 0.2 Mb to a maximum of 2 Mb in all three-gene pyramided lines. The two and three blast resistance genes pyramided lines, i.e., SS30-24-82, SS30-24-73, and SS30-24-46, displayed a high level of blast resistance and submergence tolerance. The successful use of marker-assisted backcrossing strategy coupled with phenotypic selection helped in the development of Swarna-Sub1 lines having multiple blast resistance genes with superior agro-morphological and grain quality traits, which could serve as valuable genetic stocks in the rice breeding program.


Blast resistance Background selection Foreground selection Marker-assisted selection Swarna-Sub1 



The study is part of the Ph.D. research of the first author. We thank the University Grants Commission for the financial assistance under Rajiv Gandhi National Fellowship Scheme and Indian Institute of Rice Research, Hyderabad, for providing field and laboratory facilities.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

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Supplementary file 4 (DOC 847 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Parashuram Patroti
    • 1
  • B. Vishalakshi
    • 2
  • B. Umakanth
    • 2
  • J. Suresh
    • 3
  • P. Senguttuvel
    • 2
  • M. Sheshu Madhav
    • 2
    • 4
    Email author
  1. 1.ICAR-Indian Institute of Millets Research, Regional StationSolapurIndia
  2. 2.Division of Molecular Biology and Biotechnology, Crop Improvement SectionIndian Institute of Rice ResearchHyderabadIndia
  3. 3.Division of Genetics and Plant BreedingProfessor Jayashankar Telangana State Agricultural UniversityHyderabadIndia
  4. 4.Department of BiotechnologyICAR-Indian Institute of Rice ResearchRajendranagar, HyderabadIndia

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