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Euphytica

, 215:90 | Cite as

The Rx gene derived USDA 41956 and Rx1 gene derived CPC 1673 confer equal resistance to the migration of Potato virus X from potato leaves to tubers

  • Kengo OhbayashiEmail author
Article
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Abstract

Genes conferring resistance to Potato virus X (PVX) are known, but it remains unknown whether these genes also confer resistance to the migration of PVX from potato leaves to tubers. Potato leaves of 43 cultivars and breeding lines were inoculated with PVXo and PVXb strains and their subsequent appearance in tubers was assessed. Then, a sequence-characterized amplified region (SCAR) marker was developed, and the nucleotide sequence of the SCAR marker was compared with the sequences of known PVX resistance genes. Eleven cultivars and breeding lines exhibited resistance to viral migration from leaves to tubers. The SCAR marker was detected in all 11 potato cultivars and breeding lines that exhibited resistance to both PVXo and PVXb migration, as well as an additional six lines that had not been screened. Comparisons of the nucleotide sequences of the SCAR markers from Atlantic, Multa, Saco, and USDA 41956 revealed a sequence similarity of 100% with Rx1 resistance gene derived from Solanum tuberosum ssp. andigena CPC 1673 for all cultivars and breeding lines and a sequence similarity of 99.5% with the Rx2 resistance gene derived from Solanum acaule. The Rx gene was inherited as immune resistance by a single dominant gene. Within the SCAR marker, Rx had a higher sequence similarity with Rx1 than with Rx2 and it was suggested that Rx and Rx1 are the same gene derived from the S. tuberosum ssp. andigena. Introducing Rx or Rx1 into cultivars to obtain PVX free potato tubers is effective in potato breeding.

Keywords

Potato virus X Tuber migration resistance USDA 41956 CPC 1673 Rx Rx1 

Notes

Acknowledgements

I thank R. Yoshii and Y. Noguchi, former part-time staff of the Nagasaki Agricultural and Forestry Technical Development Center, and N. Sakaiyama, T. Nakao, and M. Higuchi, former students of Nagasaki Agricultural College, for their work on material management to support the experimental research. I also thank M. Sato and H. Shiina of the Center for Seeds and Seedlings, NARO, for help with the PVX resistance test. I was guided in this study by K. Komura, former staff member of Nagasaki Agricultural and Forestry Technical Development Center. Finally, this work was also supported by the staff of the Potato Laboratory of Agriculture and Horticulture Research Division, Nagasaki Agricultural and Forestry Technical Development Center.

References

  1. Ahmadvand R, Wolf I, Gorji AM, Polgár Z, Taller J (2013) Development of molecular tools for distinguishing between the highly similar Rx1 and Rx2 PVX extreme resistance genes in tetraploid potato. Potato Res 56:277–291CrossRefGoogle Scholar
  2. Amin H, El-Sayed OE, El-Dougdoug K, Othman B, Gomaa MAM (2009) PCR-detection of Ry and Rx genes from potato with potential for wide applications in plants. Genes Genomes Genomics 3:80–88Google Scholar
  3. Bendahmane A, Kanyuka K, Baulcombe DC (1997) High-resolution genetical and physical mapping of the Rx gene for extreme resistance to Potato virus X in tetraploid potato. Theor Appl Genet 95:153–162CrossRefGoogle Scholar
  4. Bendahmane A, Kanyuka K, Baulcombe DC (1999) The Rx gene from potato controls separate virus resistance and cell death responses. Plant Cell 11:781–791CrossRefGoogle Scholar
  5. Bendahmane A, Querci M, Kanyuka K, Baulcombe DC (2000) Agrobacterium transient expression system as a tool for the isolation of disease resistance genes: application to the Rx2 locus in potato. Plant J 21(1):73–81CrossRefGoogle Scholar
  6. Bradshaw JE (2009) Potato breeding at the Scottish Plant breeding station and the Scottish Crop Research Institute: 1920–2008. Potato Res 52:141–172CrossRefGoogle Scholar
  7. Bradshaw JE, Mackey GR (1994) Potato genetics. In: Swiezynski KM (ed) Inheritance of resistance to virus. CAB International, Cambridge, pp 348–350Google Scholar
  8. Chase SS (1963) Analytic breeding in Solanum tuberosum L.—a scheme utilizing parthenotes and other diploid stocks. Can J Genet Cytol 5:359–363CrossRefGoogle Scholar
  9. Cockerham G (1955) Strains of Potato virus X. In: Proceedings of the second conference of potato virus disease, Lisse, Wageningen, pp 89–92Google Scholar
  10. Cockerham G (1970) Genetical studies on resistance to potato viruses X and Y. Heredity 25(3):309–348CrossRefGoogle Scholar
  11. Corsini DL, Pavek JJ, Martin MW, Brown CR (1994) Potato germplasm with combined resistance to leafroll virus and viruses X and Y. Am Potato J 71:377–385CrossRefGoogle Scholar
  12. Cunningham CE, Akeley RV, Peterson LC, Snyder TE (1968) Wauseon: a new potato variety resistant to golden nematode with good processing quality. Am Potato J 45:146–149CrossRefGoogle Scholar
  13. Gebhardt C, Bellin D, Henselewski H, Lehmann W, Schwarzfischer J, Valkonen JP (2006) Marker-assisted combination of major genes for pathogen resistance in potato. Theor Appl Genet 112:1458–1464CrossRefGoogle Scholar
  14. Goth RW, Webb RE (1985) Detection and distribution of latent viruses in the potato cultivar Atlantic. Plant Dis 69(10):851–853Google Scholar
  15. Halterman D, Charkowski A, Verchot J (2012) Potato, viruses, and seed certification in the USA to provide healthy propagated tubers. Pest Technol 6:1–14Google Scholar
  16. Hermsen JGTH, Verdenius J (1973) Selection from Solanum tuberosum group phureja of genotypes combining high-frequency haploid induction with homozygosity for embryo-spot. Euphytica 22:244–259CrossRefGoogle Scholar
  17. Huisman MJ, Linthorst HJM, Bol JF, Cornelissen BJ (1988) The complete nucleotide sequence of Potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. J Gen Virol 69:1789–1798CrossRefGoogle Scholar
  18. Iketani S, Senda K, Iritani M, Itoh T, Sekiguchi K, Ohnami M, Fujita R (2015) Breeding of a new table potato variety “Saya-akane” with high resistance to Phytophthora infestans and high quality. Breed Res 17:25–34CrossRefGoogle Scholar
  19. Iuchi M, Ogawa J (2000) The determination and seed tuber production of variety of potato in Tokushima prefecture. Tokushima Agric Exp Stn Rep 36:7–17Google Scholar
  20. Jones RAC (1982) Breakdown of Potato virus X resistance gene NX: selection of a group four strain from strain group three. Plant Pathol 31:325–331CrossRefGoogle Scholar
  21. Kagiwada S, Yamaji Y, Nakabayashi H, Ugaki M, Namba S (2002) The complete nucleotide sequence of Potato virus X strain OS: the first complete sequence of a Japanese isolate. J Gen Plant Pathol 68(1):94–98CrossRefGoogle Scholar
  22. Kavanagh T, Goulden M, Santa SC, Chapman S, Barker I, Baulcombe DC (1992) Molecular analysis of a resistance-breaking strain of Potato virus X. Virology 189(2):609–617CrossRefGoogle Scholar
  23. Kobayashi A, Mori M, Takada A, Tsuda S, Takada N, Umemura Y, Nakao T, Yoshida T, Kimura T, Maida T (2002) Breeding of Yukirasha: common scab-resistant potato variety for table stock. Breed Sci 52:327–332CrossRefGoogle Scholar
  24. Konieczny A, Ausubel FM (1993) A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J 4(2):403–410CrossRefGoogle Scholar
  25. Malcuit I, de Jong W, Baulcombe DC, Shields DC, Kavanagh TA (2000) Acquisition of multiple virulence/avirulence determinants by Potato virus X (PVX) has occurred through convergent evolution rather than through recombination. Virus Genes 20(2):165–172CrossRefGoogle Scholar
  26. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832CrossRefGoogle Scholar
  27. Miura T (1996) Varieties of field crops in Hokkaido. Misc Pub Hokkaido Prefect Agric Exp Stn 26:59–68Google Scholar
  28. Nakao T, Mukoujoma N, Mori K, Ishibashi Y, Chyaya M, Mori M (2004) A new potato variety “Aiyutaka”. Bull Nagasaki Agric For Exp Stn 30:1–28Google Scholar
  29. Nori M, Nishibe Y (1987) Cold field crop genetic resource information No.4. Misc Pub Hokkaido Natl Agric Exp Stn 33:102–106Google Scholar
  30. Nori M, Takada A, Kobayashi A, Tsuda S, Endo C, Umemura Y, Takada N, Maida T, Kimura T, Nakao T, Yoshida T, Momota Y, Kushida A, Uehara K, Shiina R, Hayashi K (2009) Breeding of colored potato varieties “Kitamurasaki”, “Northan Ruby”, “Shadow Queen”. Breed Res 11:145–153Google Scholar
  31. Nyalugwe EP, Wilson CR, Coutts BA, Jones RAC (2012) Biological properties of Potato virus X in potato: effects of mixed infection with Potato virus S and resistance phenotypes in cultivars from three continents. Plant Dis 96(1):43–54CrossRefGoogle Scholar
  32. Ohbayashi K, Komura K (2001) Breeding of potato parental line with pest combined resistance. Kyushu Agric Res 63:27Google Scholar
  33. Orman BE, Celnik RM, Mandel AM, Torres HN, Mentaberry AN (1990) Complete cDNA sequence of a South American isolate of Potato virus X virus. Res Virus 16(3):293–305CrossRefGoogle Scholar
  34. Ottoman RJ, Hane DC, Brown CR, Yilma S, James SR, Mosley AR, Crosslin JM, Vales MI (2009) Validation and implementation of marker-assisted selection (MAS) for PVY resistance (Ry adg gene) in a tetraploid potato breeding program. Am J Potato Res 86:304–314CrossRefGoogle Scholar
  35. Palukaitis P (2012) Resistance to viruses of potato and their vectors. Plant Pathol J 28(3):248–258CrossRefGoogle Scholar
  36. Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993CrossRefGoogle Scholar
  37. Querci M, van der Vlugt R, Goldbach R, Salazar LF (1993) RNA sequence of Potato virus X strain HB. J Gen Virol 74:2251–2255CrossRefGoogle Scholar
  38. Querci M, Baulcombe DC, Goldbach RW, Salazar LF (1995) Analysis of the resistance-breaking determinants of Potato virus X (PVX) strain HB on different potato genotypes expressing extreme resistance to PVX. Phytopatology 85(9):1003–1010CrossRefGoogle Scholar
  39. Ritter E, Debener T, Barone A, Salamini F, Gebhardt C (1991) RFLP mapping on potato chromosomes of two genes controlling extreme resistance to Potato virus X (PVX). Mol Gen Genet 227:81–85CrossRefGoogle Scholar
  40. Ross H (1986) Potato breeding-problems and perspectives. Verlag Paul Parey, Berlin, pp 72–73Google Scholar
  41. Sanetomo R, Hosaka K (2011) A maternally inherited DNA marker, descended from Solanum demissum (2n = 6x = 72) to S. tuberosum (2n = 4x = 48). Breed Res 61:426–434CrossRefGoogle Scholar
  42. Sato M, Hataya T, Iwasaki M (2000) Detection of viruses in dormant potato tubers by RT-PCR. Annu Rep Plant Prot North Jpn 51:87–92Google Scholar
  43. Senda K, Itoh T, Sekiguchi K, Murakami N, Okuyama Y, Iritani M, Matsunaga H (1998) A new potato variety “Hanashibetsu”. Bull Hokkaido Prefect Agric Exp Stn 74:1–17Google Scholar
  44. Skryabin KG, Kraev AS, Yu M, Rozanov MN, Chernov BK, Lukasheva LI, Atabekov JG (1988) The nucleotide sequence of Potato virus X RNA. Nucleic Acids Res 16(22):10929–10930CrossRefGoogle Scholar
  45. Takeuchi T, Nagao A, Minamihashi A, Shirai S (2014) Varieties of field crops in Hokkaido. Misc Pub Hokkaido Prefect Agric Exp Stn 416:38–39Google Scholar
  46. Tanaka S (1980) Experiments on resistance to Potato virus X, A and Y on potato varieties. Res Bull Natl Potato Found Stock Seed Farm 14:1–16Google Scholar
  47. Tanaka T, Komura K (2000) Development of a genetic diagnosis technique for detection of resistant feature to Globodera rostochiensis in potato (1). Bull Nagasaki Agric For Exp Stn 26:1–18Google Scholar
  48. Van der Voort JR, Kanyuka K, van der Vossen E, Bendahmane A, Mooijman P, Lankhorst RK, Stiekema W, Baulcombe DC, Bakker J (1999) Tight physical linkage of the nematode resistance gene Gpa2 and the virus resistance gene Rx on a single segment introgressed from the wild species Solanum tuberosum subsp. andigena CPC 1673 into cultivated potato. Mol Plant Microbe Interact 12(3):197–206CrossRefGoogle Scholar
  49. Webb RE, Wilson DR, Shumaker JR, Graves B, Henninger MR, Watts J, Frank JA, Murphy HJ (1978) Atlantic: a new potato variety with high solids, good processing quality, and resistance to pests. Am Potato J 55:141–145CrossRefGoogle Scholar
  50. Wilson CR, Jones RAC (1995) Occurrence of Potato virus X strain group 1 in seed stocks of potato cultivars lacking resistance genes. Ann Appl Biol 127:479–487CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Nagasaki Agricultural and Forestry Technical Development CenterIsahayaJapan

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