, 215:32 | Cite as

Response of quantitative and physiological traits to drought stress in the SeriM82/Babax wheat population

  • Neda Sobhaninan
  • Bahram HeidariEmail author
  • Sirous Tahmasebi
  • Ali Dadkhodaie
  • C. Lynne McIntyre


Assessment of quantitative traits in bi-parental progenies provides a strategy to identify drought tolerant genotypes and stress-adaptive traits in wheat. A recombinant inbred line (RIL) population with 167 lines produced from the SeriM82/Babax cross was subjected to normal irrigation and drought stress (at the heading stage) under field conditions. The study was done in Bajgah, Shiraz, Fars province, Iran, during three consecutive seasons. Shiraz is located in a temperate, major wheat production region of Iran that is prone to periodic drought. The aims of this study were to identify drought tolerant RILs and drought adaptive traits. The mean grain yield was 237.91 and 148.60 g m−2 under normal irrigation and drought stressed conditions, respectively. Above 48.0% reduction in grain yield was observed under drought stress in the three consecutive growing seasons. Several RIL lines had high grain yield under both moisture regimes. The mean leaf relative water content (RWC) was 0.4 under drought condition. One RIL had relatively high RWC and grain yield under both treatments. Heritability of several traits did not change between the two moisture conditions whereas it was reduced for few of traits. Assessment of the interrelationship of traits showed that RWC, days to heading, awn length and spikelets per spike were the main contributors to grain yield that became more important under drought conditions. Given the relatively high heritability values estimated under both conditions and strong association with grain yield, these drought adaptive traits could be used for indirect selection to improve drought tolerance in this region of Iran. Overall, information of the identified drought adaptive traits and RILs lay foundation for the improvement of drought tolerance in wheat.


Drought Grain yield Path analysis Recombinant inbred line Wheat 



The authors gratefully acknowledge Dr. Matthew P. Reynolds and Marta S. Lopes of CIMMYT, and Dr. Lynne McIntyre of CSIRO Agriculture and Food for providing seed and information on the Seri M82/Babax population.


  1. Ahmadi A, Emam Y, Pessarakli M (2010) Biochemical changes in maize seedlings exposed to drought stress conditions at different nitrogen levels. J Plant Nutr 33:541–556CrossRefGoogle Scholar
  2. Ayer DK, Sharma A, Ojha BR, Paudel A, Dhakal K (2017) Correlation and path coefficient analysis in advanced wheat genotypes. SAARC J Agric 15:1–12CrossRefGoogle Scholar
  3. Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficits in leaves. Aust J Biol Sci 15:413–428CrossRefGoogle Scholar
  4. Blum A, Sullivan CY, Nguyen H (1997) The effect of plant size on wheat response to agents of drought stress. II. Water deficit, heat and ABA. Funct Plant Biol 24:43–48CrossRefGoogle Scholar
  5. Bogale A, Tesfaye K, Geleto T (2011) Morphological and physiological attributes associated to drought tolerance of Ethiopian durum wheat genotypes under water deficit condition. J Biodivers Environ Sci 1:22–36Google Scholar
  6. Chapman SC, Crossa J, Edmeades GO (1997) Genotype by environment effects and selection for drought tolerance in tropical maize. I. Two mode pattern analysis of yield. Euphytica 95:01–09CrossRefGoogle Scholar
  7. Clarke JM, McCAIG TN (1982) Excised-leaf water retention capability as an indicator of drought resistance of Triticum genotypes. Can J Plant Sci 62:571–578CrossRefGoogle Scholar
  8. Fellahi Z, Hannachi A, Bouzerzour H, Boutekrabt A (2013) Correlation between traits and path analysis coefficient for grain yield and other quantitative traits in bread wheat under semi arid conditions. J Agric Sust 3(1):16–26Google Scholar
  9. Fernandez GC (1992) Effective selection criteria for assessing plant stress tolerance. Adapt Food Crops Temp Water Stress 13:181992257270Google Scholar
  10. Fischer RA (1985) Physiological limitations to producing wheat in semitropical and tropical environments and possible selection criteria. In Symposium on wheats for more tropical environments, Mexico, DF (Mexico), 24–28 Sept 1984. CIMMYTGoogle Scholar
  11. Gelalcha S, Hanchinal RR (2013) Correlation and path analysis in yield and yield components in spring bread wheat (Triticuma estivum L.) genotypes under irrigated condition in Southern India. Afr J Agric Res 8(24):3186–3192Google Scholar
  12. Gupta NK, GuptaS KA (2001) Effect of water stress on physiological attributes and their relationship with growth and yield of wheat cultivars at different stages. J Agron Crop Sci 186:55–62CrossRefGoogle Scholar
  13. Holland JB, Nyquist WE, Cervantes-Martínez CT (2003) Estimating and interpreting heritability for plant breeding: an update. Plant Breed Rev 22:9–112Google Scholar
  14. Kearsey M, Pooni HS (2004) The genetical analysis of quantitative traits, 2nd edn. Chapman and Hall, Boca RatonGoogle Scholar
  15. Khamssi NN, Najaphy A (2012) Comparison of photosynthetic components of wheat genotypes under rain-fed and irrigated conditions. Photochem Photobiol 88:76–80. PubMedCrossRefGoogle Scholar
  16. Law CN, Snape JW, Worland AJ (1978) The genetical relationship between height and yield in wheat. Heredity 40:133. CrossRefGoogle Scholar
  17. Leilah AA, Al-Khateeb SA (2005) Statistical analysis of wheat yield under drought conditions. J Arid Environ 61:483–496CrossRefGoogle Scholar
  18. Lopes MS, Reynolds MP, Jalal-Kamali MR, Moussa M, Feltaous Y, Tahir ISA, Barma N, Vargas M, Mannes Y, Baum M (2012) The yield correlations of selectable physiological traits in a population of advanced spring wheat lines grown in warm and drought environments. Field Crops Res 128:129–136CrossRefGoogle Scholar
  19. Lopes MS, Reynolds MP, McIntyre CL, Mathews KL, Kamali MJ, Mossad M, Feltaous Y, Tahir IS, Chatrath R, Ogbonnaya F, Baum M (2013) QTL for yield and associated traits in the Seri/Babax population grown across several environments in Mexico, in the West Asia, North Africa, and South Asia regions. Theor Appl Genet 126:971–984PubMedCrossRefGoogle Scholar
  20. Mathews KL, Malosetti M, Chapman S, McIntyre L, Reynolds M, Shorter R, van Eeuwijk F (2008) Multi-environment QTL mixed models for drought stress adaptation in wheat. Theor Appl Genet 117:1077–1091PubMedCrossRefGoogle Scholar
  21. McIntyre CL, Mathews KL, Rattey A, Chapman SC, Drenth J, Ghaderi M, Reynolds M, Shorter R (2010) Molecular detection of genomic regions associated with grain yield and yield-related components in an elite bread wheat cross evaluated under irrigated and rainfed conditions. Theor Appl Genet 120:527–541PubMedCrossRefGoogle Scholar
  22. Mir RR, Zaman-Allah M, Sreenivasulu N, Trethowan R, Varshney RK (2012) Integrated genomics, physiology and breeding approaches for improving drought tolerance in crops. Theor Appl Genet 125:625–645PubMedPubMedCentralCrossRefGoogle Scholar
  23. Mohammadi R, Abdulahi A (2017) Evaluation of durum wheat genotypes based on drought tolerance indices under different levels of drought stress. J Agric Sci 62:1–14Google Scholar
  24. Mohammadi R, Haghparast R, Amri A, Ceccarelli S (2010) Yield stability of rainfed durum wheat and GGE biplot analysis of multi-environment trials. Crop Past Sci 61:92–101CrossRefGoogle Scholar
  25. Olivares-Villegas JJ, Reynolds MP, McDonald GK (2007) Drought-adaptive attributes in the Seri/Babax hexaploid wheat population. Funct Plant Biol 34:189–203CrossRefGoogle Scholar
  26. Ortiz R, SayreKD GB, Gupta R, Subbarao GV, Ban T, Hodson Dixon JM, Ortiz-Monasterio JI, Reynolds M (2008) Climate change: can wheat beat the heat? Agric Ecosyst Environ 126:46–58CrossRefGoogle Scholar
  27. Payne RW, Harding SA, Murray DA, Soutar DM, Bird DB (2012) The 12 guide to the GenStat Release 15. VSN International, Hemel HempsteadGoogle Scholar
  28. Pinto RS, Reynolds MP, Mathews KL, McIntyre CL, Olivares-Villegas JJ, Chapman SC (2010) Heat and drought adaptive QTL in a wheat population designed to minimize confounding agronomic effects. Theor Appl Genet 121:1001–1021PubMedPubMedCentralCrossRefGoogle Scholar
  29. Pinto RS, Lopes MS, Collins NC, Reynolds MP (2016) Modelling and genetic dissection of staygreen under heat stress. Theor Appl Genet 129(11):2055–2074PubMedPubMedCentralCrossRefGoogle Scholar
  30. Pradhan GP, Prasad PV, Fritz AK, Kirkham MB, Gill BS (2012) Effects of drought and high temperature stress on synthetic hexaploid wheat. Funct Plant Biol 39:190–198CrossRefGoogle Scholar
  31. Rattey A, Shorter R, Chapman S, Dreccer F, van Herwaarden A (2009) Variation for and relationships among biomass and grain yield component traits conferring improved yield and grain weight in an elite wheat population grown in variable yield environments. Crop Past Sci 60:717–729CrossRefGoogle Scholar
  32. Reynolds M, Manes Y, Izanloo A, Langridge P (2009) Phenotyping approaches for physiological breeding and gene discovery in wheat. Ann Appl Biol 155(3):309–320CrossRefGoogle Scholar
  33. Reynolds M, Manes Y, Rebetzke G (2012) Application of physiology in breeding for heat and drought stress. In Reynolds M, Pask A, Mullan D (eds) Physiological breeding. I: Interdisciplinary approaches to improve crop adaptation. CIMMYT, MexicoGoogle Scholar
  34. Riaz R, Chowdhry MA (2003) Genetic analysis of some economic traits of wheat under drought condition. Asian J Plant Sci 2:790–796CrossRefGoogle Scholar
  35. Saini HS, Westgate ME (1999) Reproductive development in grain crops during drought. Adv Agric 68:59–96CrossRefGoogle Scholar
  36. SAS (2004) SAS version 9.2. SAS Institute Inc SAS Online Doc 913 SAS Institute Inc., Cary, NCGoogle Scholar
  37. Siddique KHM, Belford RK, Perry MW, Tennant D (1989) Growth, development and light interception of old and modern wheat cultivars in a Mediterranean-type environment. Aust J Agric Res 40:473–487Google Scholar
  38. SohrabiChah-Hassan F, Soluki M, Fakhri BA, Masoudi B (2018) Mapping QTLs for physiological and biochemical traits related to grain yield under control and terminal heat stress conditions in bread wheat (Triticum aestivum L.). Physiol Mol Biol Plants 24(6):2Google Scholar
  39. Tahmasebi S, Heidari B, Pakniyat H, Jalal Kamali MR (2014) Independent and combined effects of heat and drought stress in the SeriM82 × Babax bread wheat population. Plant Breed 133:702–711CrossRefGoogle Scholar
  40. Tahmasebi S, Heidari B, Pakniyat H, Dadkhodaie A (2015) Consequences of 1BL/1RS translocation on agronomic and physiological traits in wheat. Cereal Res Commun 43:554–566CrossRefGoogle Scholar
  41. Tahmasebi S, Heidari B, Pakniyat H, McIntyre CLM (2017) Mapping QTLs associated with agronomic and physiological traits under terminal drought and heat stress conditions in wheat (Triticum aestivum L.). Genome 60:26–45PubMedCrossRefGoogle Scholar
  42. Tammam AA, Alhamd MA, Hemeda MM (2008) Study of salt tolerance in wheat (Triticum aestivum L.) cultivar Banysoif 1. Aust J Crop Sci 1:115–125Google Scholar
  43. Teulat B, Zoumarou-Wallis N, Rotter B, Salem MB, Bahri H, This D (2003) QTL for relative water content in field-grown barley and their stability across Mediterranean environments. Theor Appl Genet 108:181–188PubMedCrossRefGoogle Scholar
  44. Trethowan RM, Reynolds M (2007) Drought resistance: genetic approaches for improving productivity under stress. In: Buck HD, Nisi JE, Salomon N (eds) Wheat production in stressed environments. Springer, Berlin, pp 289–299CrossRefGoogle Scholar
  45. Tricker PJ, Ehabti A, Schmidt J, Fleury P (2018) The physiological and genetic basis of combined drought and heat tolerance in wheat. J Exp Bot 69(13):3195–3210PubMedCrossRefGoogle Scholar
  46. Villareal RL, Bañuelos O, Mujeeb-Kazi A, Rajaram S (1998) Agronomic performance of chromosomes 1B and T1BL. 1RS near-isolines in the spring bread wheat Seri M82. Euphytica 103:195–202CrossRefGoogle Scholar
  47. Villegas D, Aparicio N, Blanco R, Royo C (2001) Biomass accumulation and main stem elongation of durum wheat grown under Mediterranean conditions. Ann Bot 88:617–627CrossRefGoogle Scholar
  48. Wardlaw IF, Willenbrink J (1994) Carbohydrate storage and mobilisation by the culm of wheat between heading and grain maturity: the relation to sucrose synthase and sucrose-phosphate synthase. Funct Plant Biol 21:255–271CrossRefGoogle Scholar
  49. Xue GP, McIntyre CL, Jenkins CLD, Glassop D, van Herwaarden AF, Shorter R (2008) Molecular dissection of variation in carbohydrate metabolism related to water-soluble carbohydrate accumulation in stems of wheat. Plant Physiol 146:441–454PubMedPubMedCentralCrossRefGoogle Scholar
  50. Yadav RS, Hash CT, Bidinger FR, Devos KM, Howarth CJ (2004) Genomic regions associated with grain yield and aspects of post-flowering drought tolerance in pearl millet across stress environments and tester background. Euphytica 136:265–277CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Crop Production and Plant Breeding, School of AgricultureShiraz UniversityShirazIran
  2. 2.Seed and Plant Improvement Department, Fars Agriculture and Natural Resources, Research and Education CenterAREEODarabIran
  3. 3.CSIRO AgricultureQueensland Bioscience PrecinctSt. LuciaAustralia

Personalised recommendations