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Euphytica

, 215:20 | Cite as

Genetic analysis and mapping of a striped rind gene (st3) in melon (Cucumis melo L.)

  • Liu Liu
  • Tingting Sun
  • Xiangyu Liu
  • Yu Guo
  • Xin Huang
  • Peng GaoEmail author
  • Xuezheng WangEmail author
Article
  • 187 Downloads

Abstract

As an important commodity trait in melons, the rind is the most direct standard of evaluation for consumers. In this study, we obtained an F2 melon population derived from self-crossing an F1 generation from a cross between X010 (green rind with stripes) and M1-113 (white rind without stripes) and performed genetic analysis and mapping. Genetic analysis revealed that a single dominant gene (st3) controlled the striped rind trait in melons. According to bulked segregant analysis, two DNA bulks were constructed (20 striped and 20 solid-colored rind plants). Next-generation sequencing was used on two parental lines (X010 and M1-113) and two DNA bulks. The st3 gene was identified on chromosome 4 within 2.7 Mbp region. Fourty cleaved amplified polymorphic sequences (CAPS) markers were developed, and sixteen CAPS markers with polymorphisms on chromosome 4 were used for constructing a genetic linkage map, and identifying recombinants in a large F2 population with six new CAPS markers. The location of the st3 gene was narrowed to 172.8 kbp on chromosome 4 between markers M-4-28 (825,401 bp) and M-4-27 (998,261 bp). This study provides a theoretical basis for the fine mapping, cloning and functional characterization of st3.

Keywords

Melon Rind stripes Bulked segregant analysis CAPS Mapping 

Notes

Acknowledgments

Authors highly acknowledged the funding granted by “The National Nature Science Foundation of China” (Grant No. 31672177), the “Academic Backbone” Project of Northeast Agricultural University (Grant No. 16XG06), the “Young Talents” Project of Northeast Agricultural University (Grant No. 14QC09), Heilongjiang Province Collegial Young Innovative Talent Training Program (Grant No. UNPYSCT-2016136), and “The National Nature Science Foundation of China” (Grant No. 31372028).

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region)Ministry of AgricultureHarbinChina
  2. 2.Horticulture and Landscape Architecture of Northeast Agricultural UniversityHarbinChina
  3. 3.Heilongjiang Agricultural Economy Vocational CollegeMudanjiangChina

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