Evaluation of anaerobic germinability in various rice subpopulations: identifying genotypes suitable for direct-seeded rice cultivation
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Anaerobic conditions in waterlogged soil lead to low germination rates, which significantly reduce crop yields. Good seed germination is prerequisite for direct-seeded cultivation of rice to obtain optimal yields not only in irrigated lowland but most importantly in rain-fed and waterlogged areas where water supply could be more than needed. Due to the limited availability of rice genotypes suitable for anaerobic germination, there is an urgent need to select diverse rice germplasm with enhanced anaerobic germinability. In this study, we evaluated 185 rice accessions from six subpopulations for germination rate (AGR) and coleoptile length (ACL) under anaerobic conditions. The highest average AGR (60%) and longest average ACL (2.13 cm) were observed in tropical japonica (TRJ) and temperate japonica (TEJ) germplasm, respectively. Meanwhile, the highest proportion of accessions with “very long” ACL was in the TEJ subpopulation, whereas those with the “highest” AGR were in the aus subpopulation based on our criteria. We selected seven strong accessions for anaerobic germinability (AG) based on AGR and ACL and analyzed the relative expression patterns of four AG-related genes in strong and weak accessions via qRT-PCR. In general, proton pyrophosphatase locus (OVP3) was expressed at the highest levels in strong accessions, whereas the expression level of rice ethylene response element binding protein locus (EREBP1) did not significantly differ among accessions under normal and anaerobic conditions. The relative expression results of rice alpha amylase locus (RAmy3D) and OVP3 showed distinct patterns and divided all strong accessions into two groups, suggesting that major genes involved in AG may vary depending on the germplasm. These findings could be helpful for breeders and lay the foundation for further genetic analysis.
KeywordsAnaerobic germination Rice subpopulation Evaluation Gene expression Direct-seeded rice
This study was supported by the “Research Program for Agricultural Science and Technology Development (Project No. PJ010871)” of the National Institute of Agricultural Sciences, RDA.
- Civáň P, Craig H, Cox CJ, Brown TA (2015) Three geographically separate domestications of Asian rice. Nat Plants 1:1–5Google Scholar
- Jiang L, Hou M, Wang C, Wan J (2004) Quantitative trait loci and epistatic analysis of seed anoxia germinability in rice (Oryza sativa L.). Rice Sci 11:238–244Google Scholar
- Ladha JK, Kumar V, Alam MM, Sharma S, Gathala M, Chandna P, Saharawat YS, Balasubramanian V (2009) Integrating crop and resource management technologies for enhanced productivity, profitability, and sustainability of the rice-wheat system in South Asia. In: Ladha JK, Singh Y, Erenstein O, Hardy B (eds) Integrated crop and resource management in the rice-wheat system of south Asia. International Rice Research Institute, Los Banos, pp 69–108Google Scholar
- McGraw-Hill C (2008) Statistix 8.1 Analytical Software, Tallahassee, Florida Maurice/Thomas text. Analytical Software, Tallahassee. ISBN 0073402818Google Scholar
- Pandey S, Velasco L (2002) Economics of direct seeding in Asia: patterns of adoption and research priorities. In: Pandey S, Mortimer M, Wade L, Tuong TP, Lopez K, Hardy B (eds) Direct seeding: research strategies and opportunities. International Rice Research Institute, Los Banos, pp 3–14Google Scholar
- Septiningsih EM, Collard BCY, Heuer S, Bailey-Serres J, Ismail AM, Mackill DJ (2013a) Applying genomics tools for breeding submergence tolerance in rice. In: Varshney RK, Tuberosa R (eds) Translational genomics for crop breeding: improvement for abiotic stress, quality and yield improvement, vol 2. Wiley-Blackwell, Hoboken, pp 9–30CrossRefGoogle Scholar
- Seshu DV, Krishnasamy V, Siddique SB (1988) Seed vigor in rice. Rice seed health. International Rice Research Institute, Los Banos, pp 315–329Google Scholar
- Takahashi H, Greenway H, Matsumura H, Tsutsumi N, Nakazono M (2014) Rice alcohol dehydrogenase 1 promotes survival and has a major impact on carbohydrate metabolism in the embryo and endosperm when seeds are germinated in partially oxygenated water. Ann Bot 113:851–859PubMedPubMedCentralCrossRefGoogle Scholar
- XLSTAT (2017) Data analysis and statistical solution for Microsoft excel. Addinsoft, ParisGoogle Scholar