, 215:19 | Cite as

Evaluation of anaerobic germinability in various rice subpopulations: identifying genotypes suitable for direct-seeded rice cultivation

  • Muhammad Rauf
  • Yu-Mi Choi
  • Sukyeung Lee
  • Myung-Chul Lee
  • Sejong Oh
  • Do Yoon HyunEmail author


Anaerobic conditions in waterlogged soil lead to low germination rates, which significantly reduce crop yields. Good seed germination is prerequisite for direct-seeded cultivation of rice to obtain optimal yields not only in irrigated lowland but most importantly in rain-fed and waterlogged areas where water supply could be more than needed. Due to the limited availability of rice genotypes suitable for anaerobic germination, there is an urgent need to select diverse rice germplasm with enhanced anaerobic germinability. In this study, we evaluated 185 rice accessions from six subpopulations for germination rate (AGR) and coleoptile length (ACL) under anaerobic conditions. The highest average AGR (60%) and longest average ACL (2.13 cm) were observed in tropical japonica (TRJ) and temperate japonica (TEJ) germplasm, respectively. Meanwhile, the highest proportion of accessions with “very long” ACL was in the TEJ subpopulation, whereas those with the “highest” AGR were in the aus subpopulation based on our criteria. We selected seven strong accessions for anaerobic germinability (AG) based on AGR and ACL and analyzed the relative expression patterns of four AG-related genes in strong and weak accessions via qRT-PCR. In general, proton pyrophosphatase locus (OVP3) was expressed at the highest levels in strong accessions, whereas the expression level of rice ethylene response element binding protein locus (EREBP1) did not significantly differ among accessions under normal and anaerobic conditions. The relative expression results of rice alpha amylase locus (RAmy3D) and OVP3 showed distinct patterns and divided all strong accessions into two groups, suggesting that major genes involved in AG may vary depending on the germplasm. These findings could be helpful for breeders and lay the foundation for further genetic analysis.


Anaerobic germination Rice subpopulation Evaluation Gene expression Direct-seeded rice 



This study was supported by the “Research Program for Agricultural Science and Technology Development (Project No. PJ010871)” of the National Institute of Agricultural Sciences, RDA.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Muhammad Rauf
    • 1
  • Yu-Mi Choi
    • 1
  • Sukyeung Lee
    • 1
  • Myung-Chul Lee
    • 1
  • Sejong Oh
    • 1
  • Do Yoon Hyun
    • 1
    Email author
  1. 1.National Agrobiodiversity CenterNational Institute of Agricultural Sciences, RDAJeonjuRepublic of Korea

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