, 214:219 | Cite as

Genetics of Fe, Zn, β-carotene, GPC and yield traits in bread wheat (Triticum aestivum L.) using multi-locus and multi-traits GWAS

  • Jitendra Kumar
  • Gautam Saripalli
  • Vijay Gahlaut
  • Neha Goel
  • Prabina Kumar Meher
  • Kaushlesh Kumar Mishra
  • Prafulla Chandra Mishra
  • Deepmala Sehgal
  • Prashant Vikram
  • Carolina Sansaloni
  • Sukhwinder SinghEmail author
  • Pradeep Kumar Sharma
  • Pushpendra Kumar GuptaEmail author


The present study was conducted to study the genetic architecture of grain micronutrients (Zn, Fe and β-carotene contents), grain protein content and four yield traits in a spring wheat reference set comprising 246 genotypes. Phenotypic data on these traits recorded at two locations and the genotyping data for 17,937 SNP markers (obtained through outsourcing) were used for genome wide association study, which gave following results after Bonferroni correction using four methods: (1) single locus single trait analysis gave 136 marker-trait associations; (2) multi-locus mixed model gave 587 MTAs; (3) multi-trait mixed model gave 28 MTAs and (4) matrix-variate linear mixed model gave 33 MTAs. As many as 73 epistatic interactions were also detected. Keeping all the results in mind, nine most important MTAs were selected for biofortification. These markers were associated with three traits (GPC, GFeC and GYPP). These MTAs can be used in wheat improvement programs either using marker-assisted recurrent selection or pseudo-backcrossing method.


Bread wheat (Triticum aestivum L.) GWAS SLST MLMM MTMM mvLMM 



JK received financial support in the form of a Senior Research Fellowship (SRF) from Indian Council of Medical Research (ICMR). Head, Genetics and Plant Breeding, CCS University, Meerut provided the facilities and Prof. V.K. Mishra, Banaras Hindu University Varanasi, India provided facility for zinc and iron estimations. CIMMYT gene bank is sincerely acknowledged for providing seed material and genotypic data for the present research.

Supplementary material

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Jitendra Kumar
    • 1
  • Gautam Saripalli
    • 1
  • Vijay Gahlaut
    • 1
  • Neha Goel
    • 1
  • Prabina Kumar Meher
    • 2
  • Kaushlesh Kumar Mishra
    • 3
  • Prafulla Chandra Mishra
    • 3
  • Deepmala Sehgal
    • 4
  • Prashant Vikram
    • 4
  • Carolina Sansaloni
    • 4
  • Sukhwinder Singh
    • 4
    Email author
  • Pradeep Kumar Sharma
    • 1
  • Pushpendra Kumar Gupta
    • 1
    Email author
  1. 1.Department of Genetics and Plant BreedingCh. Charan Singh UniversityMeerutIndia
  2. 2.Centre for Agricultural BioinformaticsICAR-Indian Agricultural Statistics Research InstituteNew DelhiIndia
  3. 3.Zonal Agricultural Research Station, PowarkhedaJawaharlal Nehru Krishi Vishwa VidylayaJabalpurIndia
  4. 4.International Maize and Wheat Improvement Center (CIMMYT)TexcocoMexico

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