Identification and fine mapping of molecular markers closely linked to fruit spines size ss gene in cucumber (Cucumis sativus L.)
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Fruit spine size is one of the importantly external quality traits effected the economic value of cucumber fruit. Morphological–cytological observation of the fruit spine size phenotype indicated that large spine formation arises from an increasing of spiny pedestal cell number caused by cell division, and best periods to accurately score fruit spine size trait was 4th day before flowering to 7th day after flowering according the continuous observation. Genetic analysis showed that a single dominant gene determined the fruit spine size trait in cucumber. BC1 population (189 individuals) of two inbred lines (large spine PI197088 and small spine SA0422) was used for primary mapping of the SS/ss locus with 7 markers covering an interval of 37.1 cM. An F2 segregating population of 1032 individuals constructed from the same two parents (PI197088 and SA0422) was used to fine mapping of the SS/ss locus. Six new markers linked to the gene were successfully screened for construction of a fine linkage map, in which the SS/ss locus was located in the region flanked by marker SE1 (3 recombinants) and SSR43 (2 recombinants) with a 189 kb physical distance. Markers from this study will be valuable for candidate gene cloning and marker-assisted selection for cucumber breeding.
KeywordsCucumber Fruit external quality Fruit spine Mapping MAS
This study was funded by Shanghai Natural Science Foundation (No. 15ZR1429800), the National Natural Science Foundation of China (No. 31672148), and the Youth Fund of the Natural Science Foundation of Jiangsu Province of China (BK20150232).
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Conflict of intertest
The authors declare that they have no conflict of interest.
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