A new DNA marker for sex identification in purple asparagus
Garden asparagus (Asparagus officinalis L.) is an economically-important perennial crop. This plant is dioecious, as there are both male and female individuals; male individuals are preferred over females for agricultural production. To reduce the time required for garden asparagus breeding, various male-specific DNA markers are utilized. Male-specific DNA markers, such as Asp1-T7sp and MSSTS710, are currently available for sex identification in many asparagus cultivars. In the current study, we found that these markers are not suitable for sex identification in the purple asparagus cultivar ‘Pacific Purple’, as male-specific amplification of this marker was detected in some male individuals of this cultivar but not in other males. The Asp1-T7sp marker is suitable for use in sex identification in various Asparagus species related to A. officinalis, indicating that the region around this marker is conserved among these species. Thus, we isolated a DNA fragment around this marker by inverse PCR and produced a new DNA marker, MspHd, based on this sequence. However, like Asp1-T7sp and MSSTS710, MspHd was not suitable for sex identification in the cultivar ‘Pacific Purple’. Since all ‘Pacific Purple’ males have morphologically similar male flowers with functional stamens, we produced a new male-specific marker based on the sex determination gene, MSE1/AspMYB35/AspTDF1, which is responsible for stamen development. This marker, named AspMSD, is suitable for sex identification in ‘Pacific Purple’. In addition, this marker can be utilized for sex identification in various asparagus cultivars and some related Asparagus species.
KeywordsMale-specific marker Sex determination gene MSE1/AoMYB35/AspTDF1 ‘Pacific Purple’ Asparagus species Dioecious
We thank Ms. Yoko Kakimoto for her help with asparagus cultivation. This work was supported in part by the Matsushima Horticultural Development Foundation (Japan) and a Grant-in-Aid for Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry (No. 27002B) from the Ministry of Agriculture, Forestry and Fisheries (MAFF) (Japan).
- Harkess A, Zhou J, Xu C, Bowers JE, Van der Hulst R, Ayyampalayam S, Mercati F, Riccardi P, McKain MR, Kakrana A, Tang H, Ray J, Groenendijk J, Arikit S, Mathioni SM, Nakano M, Shan H, Telgmann-Rauber A, Kanno A, Yue Z, Chen H, Li W, Chen Y, Xu X, Zhang Y, Luo S, Chen H, Gao J, Mao Z, Pires JC, Luo M, Kudrna D, Wing RA, Meyers BC, Yi K, Kong H, Lavrijsen P, Sunseri F, Falavigna A, Ye Y, Leebens-Mack JH, Chen G (2017) The asparagus genome sheds light on the origin and evolution of a young Y chromosome. Nat Commun 8:1279CrossRefPubMedPubMedCentralGoogle Scholar
- Ii Y, Uno Y, Kanechi M, Inagaki N (2012a) Screening of sex in asparagus at early growth stages. HortTechnology 22:77–82Google Scholar
- Ii Y, Uragami A, Uno Y, Kanechi M, Inagaki N (2012b) RAPD based analysis of differences between male and female genotypes of Asparagus officinalis. Hort Sci (Prague) 39:33–37Google Scholar
- Löptien H (1979) Identification of the sex chromosome pair in asparagus (Asparagus officinalis L.). Z Pflanzenzüchtg 82:162–173Google Scholar
- Moreno R, Espejo JA, Cabrera A, Gil J (2008) Origin of tetraploid cultivated asparagus landraces inferred from nuclear ribosomal DNA internal transcribed spacers’ polymorphism. Ann Appl Biol 153:233–241Google Scholar