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Euphytica

, 214:153 | Cite as

The Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rust

  • R. A. Silva
  • L. Zambolim
  • I. S. L. Castro
  • H. S. Rodrigues
  • C. D. Cruz
  • E. T. Caixeta
Article

Abstract

The main phytosanitary problems affecting global coffee production are the fungal diseases known as rust, caused by Hemileia vastatrix Berkeley and Broome, and coffee berry disease (CBD), induced by Colletotrichum kahawae Waller and Bridge. The main disease control strategy is the use of resistant coffee cultivars. Híbrido de Timor is the most important source of resistant varieties used in breeding programs worldwide. The objective of this work was to characterize the diversity and disease resistance of 152 HdT genotypes from the germplasm collection at the Universidade Federal de Viçosa (UFV). Accessions were phenotyped with H. vastatrix races II and XXXIII. Molecular analysis was carried out with 29 random microsatellite markers or single sequence repeats (SSRs), and two SSRs associated with the CBD resistance gene Ck-1. All accessions in the germplasm collection were resistant to H. vastatrix race II, and 141 were resistant to H. vastatrix race XXXIII. Based on the presence of markers, there were 106 accessions containing the CBD resistance gene Ck-1. In the diversity study, the 152 accessions clustered into 21 different groups. A unique molecular profile (fingerprint) was determined for each individual, using 52 alleles from 22 SSR markers. The HdT germplasm of UFV was highly diverse, and included 99 accessions with multiple disease resistance genes, including the CBD resistance gene Ck-1, and others conferring resistance to H. vastatrix races II and XXXIII.

Keywords

Coffee breeding Colletotrichum kahawae Durable resistance Fingerprinting Hemileia vastatrix 

Notes

Acknowledgements

This work was financially supported by the Consórcio Brasileiro de Pesquisa e Desenvolvimento do Café–CBP&D/Café, Fundação de Pesquisa do Estado de Minas Gerais–FAPEMIG, Conselho Nacional de Pesquisas–CNPq and Instituto Nacional de Ciência de Tecnologia -INCT Café. The authors gratefully acknowledge CIFC for providing the HdT accession.

Compliance with ethical standards

Conflict of interest

The authors declared that they have no conflict of interest.

Supplementary material

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Supplementary material 1 (DOCX 30 kb)
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Supplementary material 2 (DOCX 19 kb)
10681_2018_2231_MOESM3_ESM.docx (64 kb)
Supplementary material 3 (DOCX 65 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • R. A. Silva
    • 1
    • 2
  • L. Zambolim
    • 1
    • 2
  • I. S. L. Castro
    • 2
  • H. S. Rodrigues
    • 2
  • C. D. Cruz
    • 2
    • 4
  • E. T. Caixeta
    • 2
    • 3
  1. 1.Department of PhytopathologyUniversidade Federal de ViçosaViçosaBrazil
  2. 2.Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO)Universidade Federal de ViçosaViçosaBrazil
  3. 3.Empresa Brasileira de Pesquisa Agropecuária, Embrapa CaféBrasíliaBrazil
  4. 4.Department of BiologyUniversidade Federal de ViçosaViçosaBrazil

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