Euphytica

, 214:60 | Cite as

LKF, the locus regulating large grains in the rice cultivar ‘Fusayoshi’, is identical to the loci encoding a serine/threonine protein phosphatase with Kelch motif

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Abstract

The LKF locus, which regulates grain size in the rice cultivar ‘Fusayoshi’ showing large grain, has been mapped to the proximal part of the long arm of chromosome 3. An incomplete dominant allele, Lkf, caused large grain size of Fusayoshi. The structure and function of this locus, however, have not yet been determined. In a similar position to LKF on chromosome 3, two loci, Os03g0407400 (GS3) and LOC_Os03g44500, have been already reported as loci also regulating rice grain size. The objective of the present study was to determine the nucleotide sequences of both Os03g0407400 and LOC_Os03g44500 for different alleles at the LKF locus. Results showed that only one known single nucleotide polymorphism (SNP) in exon 10 of LOC_Os03g44500 was detected between a large-grain allele (Lkf) and a small-grain allele at the LKF locus, whereas no polymorphisms in Os03g0407400. This SNP, visualized using a dCAPS marker, clearly demonstrated nearly complete co-segregation with grain length in an F2 population segregating the Lkf at LKF. Other large-grain mutant lines with large-grain alleles at the LKF locus, which originated from another cultivar ‘Gimbozu’, also showed the same SNP in exon 10 of LOC_Os03g44500. It was concluded from these results that LKF is identical to LOC_Os03g44500, and the detected SNP in exon 10, at least, which is included in Kelch-like repeat motif, could be essential for expression of the large-grain phenotype.

Keywords

dCAPS marker Grain size GS3 Serine/threonine protein phosphatase Kelch-like repeat motif Rice Single nucleotide polymorphism 

Notes

Acknowledgements

We sincerely thank to Y. Fujiwara, Y. Iguchi, K. Matsumoto and N. Ueda, for their technical assistance to this study.

Supplementary material

10681_2018_2142_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 16 kb)
10681_2018_2142_MOESM2_ESM.docx (17 kb)
Supplementary material 2 (DOCX 17 kb)

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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Biology-Oriented Science and TechnologyKindai UniversityKinokawaJapan

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