Rf4 has minor effects on the fertility restoration of wild abortive-type cytoplasmic male sterile japonica (Oryza sativa) lines
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Wild abortive (WA)-type cytoplasmic male sterility (CMS) has been exclusively used for breeding three-line hybrid indica rice, but it has not been applied for generating japonica hybrids because of the difficulties related to breeding japonica restorer lines. Determining whether the major restorer-of-fertility (Rf) gene used for indica hybrids can efficiently restore the fertility of WA-type japonica CMS lines may be useful for breeding WA-type japonica restorer lines. In this study, japonica restorer lines for Chinsurah Boro II (BT)-type CMS exhibited varying abilities to restore the fertility of ‘WA-LiuqianxinA’, which is a WA-type japonica CMS line. Additionally, Rf genes for WA-type CMS were identified in the BT-type japonica restorers. Meanwhile, ‘C9083’, which is a BT-type japonica restorer, exhibited a limited ability to restore the fertility of WA-type japonica CMS lines, and a genetic analysis revealed that the fertility restoration was controlled by one locus. The Rf gene was mapped to an approximately 370-kb physical region and was identified as Rf4. Furthermore, Rf gene dosage effects and the temperature influenced the fertility restoration of WA-type japonica CMS lines. This study is the first to confirm that Rf4 has only minor effects on the fertility restoration of WA-type japonica CMS lines. These results may be relevant for the development of WA-type japonica hybrids.
KeywordsJaponica WA-type CMS Restorer-of-fertility (Rf) gene Restoration ability Gene mapping
This study was financially supported by the National Key Research and Development Program (2016YFD0101107), the National Basic Research Program of China (2013CBA01405), and the Priority Academic Program Development of Jiangsu Higher Education Institutions. We thank Lesley Benyon, PhD, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.
HZ analyzed the data and drafted the manuscript. XC and LZ evaluated the phenotypes and analyzed the data. HS and YG helped construct the BC population. MG helped design the study. ST designed the study and revised the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Barclay A (2010) Hybridizing the world. Rice Today 9:32–35Google Scholar
- Hu J, Wang K, Huang WC, Liu G, Gao Y, Wang JM, Huang Q, Ji YX, Qin XJ, Wan L, Zhu RS, Li SQ, Yang DC, Zhu YG (2012) The rice pentatricopeptide repeat protein RF5 restores fertility in Hong-Lian cytoplasmic male-sterile lines via a complex with the glycine-rich protein GRP162. Plant Cell 24:109–122CrossRefPubMedPubMedCentralGoogle Scholar
- Huang RH (1997) Effect of different environmental factors on male-fertility stability of Longtepu A CMS lines. Fujian Sci Technol Rice Wheat 15:13–18Google Scholar
- Huang JZ, E ZG, Zhang HL, Shu QY (2014) Workable male sterility systems for hybrid rice: genetics, biochemistry, molecular biology, and utilization. Rice 7:13Google Scholar
- Huang WC, Yu CC, Hu J, Wang LL, Dan ZW, Zhou W, He CL, Zeng YF, Yao GX, Qi JZ, Zhang ZH, Zhu RS, Chen XF, Zhu YG (2015) Pentatricopeptide-repeat family protein RF6 functions with hexokinase 6 to rescue rice cytoplasmic male sterility. Proc Natl Acad Sci USA 112:14984–14989CrossRefPubMedPubMedCentralGoogle Scholar
- Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report, Whitehead Institute, CambridgeGoogle Scholar
- Tang SZ, Zhang HG, Liang GH, Yan CJ, Liu QQ, Gu MH (2008) Reasons and countermeasures of slow development on Three-line Japonica hybrid rice. Hybrid Rice 23:1–5Google Scholar
- Wang ZH, Zou YJ, Li XY, Zhang QY, Chen LT, Wu H, Su DH, Chen YL, Guo JX, Luo D, Long YM, Zhong Y, Liu YG (2006) Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. Plant Cell 18:676–687CrossRefPubMedPubMedCentralGoogle Scholar
- Yang ZY (1994) Progresses in the breeding of japonica hybrid rice. Hybrid Rice 1994(z1):46–49Google Scholar
- Yang ZY, Li ZB, Dong L, Zhu W, Cai Z, Qu LJ, Hua ZT (2016) Development and prospect of hybrid japonica rice in China. Chin Sci Bull 61:3770–3777Google Scholar
- Yuan LP (1994) Increasing yield potential in rice by exploitation of heterosis. In: Virmanni SS (ed) Hybrid rice technology. New developments and future prospects. IRRI, Manila, pp 1–6Google Scholar