QTL Identification and Fine Mapping for Seed Storability in Rice (Oryza sativa L.)
Rice seed storability is an important characteristic of seed quality so that the cultivars with strong seed storability are expected in the production of hybrid seeds. Presently, little is known about the genetic and physiological mechanisms controlling rice seed storability. In this study, a double haploid population derived from the cross between a japonica cultivar CJ06 and an indica cultivar TN1 was used to identify the quantitative trait loci (QTLs) for seed germination percentage (GP) and fatty acid content (FA) during natural storage or artificial aging. A total of 19 QTLs, including ten QTLs for GP and nine QTLs for FA, were identified on nine chromosomes with the phenotypic variations ranged from 2.1 to 22.7%. Besides, six and three pairs of epistatic interactions were identified for GP and FA, respectively. Moreover, qGP-9, a QTL for germination percentage, was delimited in an interval of 92.8 kb between two STS markers P6 and P8, which contains 15 putative open reading frames. These results provide important information for understanding the genetic mechanisms on rice seed storability, and will be useful for breeding new rice varieties with high seed storability.
KeywordsEpistatic interactions Fatty acid content QTL Rice (Oryza sativa L) Seed germination percentage
We thank Dr. D. L. Zeng, G. H. Zhang for the useful advice on designed the experiment, and thank for Dr. L. Zhu at the China National Rice Research Institute for the critical reading of the manuscript; this work was supported by grants from National Science and Technology Support Project of China (Grant No. 2011BAD35B09).
- Kim KY, Lee GM, Noh KI, Ha KY, Son JY, Kim BK, Ko JK, Kim CK (2007) Varietal difference of germination, fat acidity, and lipoxygenase activity of rice grain stored at high temperature. Kor J Crop Sci 52(1):29–35Google Scholar
- McCouch S, Cho Y, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T (1997) Report on QTL nomenclature. Rice Genet Newsl 14:1Google Scholar
- Miura K, Lin Y, Yano M, Nagamine T (2002) Mapping quantitative trait loci controlling seed longevity in rice (Oryza sativa L.). Theor Appl Genet 104(6–7):981–986Google Scholar
- Powell A, Matthews S (1984) Application of the controlled deterioration vigour test to detect seed lots of Brussels sprouts with low potential for storage under commercial conditions. Seed Sci Technol 12:649–657Google Scholar
- Ren Y, Wang Y, Liu F, Zhou K, Ding Y, Zhou F, Wang Y, Liu K, Gan L, Ma W, Han X, Zhang X, Guo X, Wu F, Cheng Z, Wang J, Lei C, Lin Q, Jiang L, Wu C, Bao Y, Wan J (2014) GLUTELIN PRECURSOR ACCUMULATION3 encodes a regulator of post-golgi vesicular traffic essential for vacuolar protein sorting in rice endosperm. Plant Cell 26(1):410–425CrossRefPubMedPubMedCentralGoogle Scholar
- Shen B, Zhuang JY, Fan YY, Ying JZ, Yang J, Sun LX, Fei JH, Zheng KL (2005) Genetic analysis of starch branching enzyme activity in rice grain. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao=J Plant Physiol. Mol Biol 31(6):631–636 (in Chinese)Google Scholar
- Xu X, Ying X, Duan B (2007) Study on determination of fatty acid value of rice. J Chin Cereals Oils Assoc 22:105–107Google Scholar
- Zeng D, Qian Q, Yasukumi K, Teng S, Hiroshi F (2002) Study on storability and morphological index in rice (Oryza sativa L.) under artificial ageing. Acta Agron Sin 28:551–554Google Scholar
- Zhang WM, Ni AL, Wang CC (1998) Study on seed vigor of hybrid rice. Seed 2:7–10Google Scholar