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Euphytica

, 213:26 | Cite as

Response to water deficit in glume of wheat: expression profiling by microarray analysis

  • Changxin Liu
  • Huicong Mi
  • Huawei Liu
  • Shuangze Xie
  • Yongjun Wu
  • Pan Ning
  • Taishuai Liang
  • Jinyin LvEmail author
Article

Abstract

To investigate the drought response in wheat spike, we evaluated variations in gene expression in the early grain-filling period at 6 days after anthesis by Affymetrix Wheat Genome Array. 566 differentially expressed probe sets were identified. 477 genes were upregulated (FC ≥ 2.0) and 89 (FC ≤ 0.5) were downregulated under water deficit. Most upregulated genes were involved in signal transduction, metabolism, and transcription. We identified signaling proteins, transcription factors, and abiotic stress-related genes among the upregulated genes. Three important genes were located at the center of the phenylalanine metabolism. This result indicates that phenylalanine metabolism and biosynthesis of flavonoids derived from carbohydrate metabolism play pivotal roles during drought-resistance response in wheat spike. Nine upregulated genes were selected for confirming gene chip results at 0, 3, 6, 10, 15, 20, and 25 DAA by real-time PCR. This study provided insights into the molecular mechanism underlying wheat spike responses against drought.

Keywords

Triticum aestivum L. Glume Water deficit Microarray analysis qRT-PCR 

Notes

Acknowledgement

This study was financially supported by Projects of the National Natural Science Foundation of China (31271624).

Compliance with ethical standards

Conflict of interest

All of the authors declare that there are no conflicts of interest.

Supplementary material

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Changxin Liu
    • 1
  • Huicong Mi
    • 1
  • Huawei Liu
    • 1
  • Shuangze Xie
    • 2
  • Yongjun Wu
    • 1
  • Pan Ning
    • 2
  • Taishuai Liang
    • 1
  • Jinyin Lv
    • 1
    Email author
  1. 1.College of Life SciencesNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.College of ScienceNorthwest A&F UniversityYanglingPeople’s Republic of China

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