Response to water deficit in glume of wheat: expression profiling by microarray analysis
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To investigate the drought response in wheat spike, we evaluated variations in gene expression in the early grain-filling period at 6 days after anthesis by Affymetrix Wheat Genome Array. 566 differentially expressed probe sets were identified. 477 genes were upregulated (FC ≥ 2.0) and 89 (FC ≤ 0.5) were downregulated under water deficit. Most upregulated genes were involved in signal transduction, metabolism, and transcription. We identified signaling proteins, transcription factors, and abiotic stress-related genes among the upregulated genes. Three important genes were located at the center of the phenylalanine metabolism. This result indicates that phenylalanine metabolism and biosynthesis of flavonoids derived from carbohydrate metabolism play pivotal roles during drought-resistance response in wheat spike. Nine upregulated genes were selected for confirming gene chip results at 0, 3, 6, 10, 15, 20, and 25 DAA by real-time PCR. This study provided insights into the molecular mechanism underlying wheat spike responses against drought.
KeywordsTriticum aestivum L. Glume Water deficit Microarray analysis qRT-PCR
This study was financially supported by Projects of the National Natural Science Foundation of China (31271624).
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Conflict of interest
All of the authors declare that there are no conflicts of interest.
- Ashraf MY, Sarwar G, Ashraf M, Naeem M (2006) Comparative ammonia and nitrate accumulation in drought tolerant and susceptible wheat genotypes under soil water deficit conditions. AGROCHIMICA 50:1–12Google Scholar
- Crismani W, Baumann U, Sutton T (2006) Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat. BMC Genom 1:1–17Google Scholar
- Franco OL, Melo FR (2000) Osmotic protectants—a plant strategy in response to osmotic stress. Russ J Plant Physiol 47:137–144Google Scholar
- Hinesley LE, Pharr DM, Snelling LK, Funderburk SR (1992) Foliar raffinose and sucrose in four conifer species: relationship to seasonal temperature. J Am Soc Hortic Sci 117:852–855Google Scholar
- Kang G, Peng X, Wang L, Yang Y, Shao R, Xie Y, Ma D, Wang C, Guo T, Zhu Y (2015) Ultrastructural observation of mesophyll cells and temporal expression profiles of the genes involved in transitory starch metabolism in flag leaves of wheat after anthesis. Physiol Plant 153:12–29CrossRefPubMedGoogle Scholar
- Marandad M, Dixon R (1989) Modulation of plant defense gene transcripts by trans-cinnamic acid. Plant Physiol 264:14486–14492Google Scholar