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Euphytica

, 213:17 | Cite as

Analysis of QTL for seed oil content in Brassica napus by association mapping and QTL mapping

  • Ying Fu
  • Dongqing Zhang
  • Madeleine Gleeson
  • Yaofeng Zhang
  • Baogang Lin
  • Shuijin Hua
  • Houdong Ding
  • Martin Frauen
  • Jiana Li
  • Wei QianEmail author
  • Huasheng YuEmail author
Article

Abstract

Increasing seed oil content is one of the most important breeding targets for rapeseed (Brassica napus). In this study, we combined quantitative trait loci (QTL) mapping and marker-trait association analysis to dissect the genetic basis of seed oil content in rapeseed. A doubled haploid (DH) population with 261 lines was grown in two highly contrasting macro-environments, Germany with winter ecotype environment and China with semi-winter ecotype environment, to explore the effect of environment effect of on seed oil content. Notable macro-environment effect was found for seed oil content. 19 QTL for seed oil content were identified across the two macro-environments. For association analysis, a total of 142 rapeseed breeding lines with diverse oil contents were grow in China macro-environment. We identified 23 simple sequence repeat (SSR) markers that were significantly associated with the seed oil content. Comparative analysis revealed that five QTL identified in the DH population, located on chromosomes A03, A09, A10 and C09, were co-localized with 11 significantly associated SSR markers that were identified from the association mapping population. Of which, the QTL on chromosome A10 was found to be homeologous with the QTL on chromosome C09 by aligning QTL confidence intervals with the reference genomes B. napus. Those QTL associated with specific macro-environments provides valuable insight into the genetic regulation of seed oil content and will facilitate marker-assisted breeding of B. napus.

Keywords

Brassica napus Environment Quantitative trait loci Marker-trait association Seed oil content 

Notes

Acknowledgements

This study was supported financially by Project of Young Researcher Cultivation of Zhejiang Academy of Agricultural Sciences; Project of Hybrid Breeding of High Oil Content in Rapeseed (2011R50026-2); The Key Project of Novel Variety Breeding of Zhejiang Province (2012C12902-1).

Authors’ contributions

HY, WQ and YF designed research; YF, DZ and HD performed research, YF, YZ, BL, and SH analyzed data; YF, MG, JL and FM wrote the paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10681_2016_1817_MOESM1_ESM.doc (216 kb)
Supplementary material 1 (DOC 216 kb)
10681_2016_1817_MOESM2_ESM.xls (176 kb)
Supplementary material 2 (XLS 176 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Ying Fu
    • 1
  • Dongqing Zhang
    • 1
  • Madeleine Gleeson
    • 3
  • Yaofeng Zhang
    • 1
  • Baogang Lin
    • 1
  • Shuijin Hua
    • 1
  • Houdong Ding
    • 1
  • Martin Frauen
    • 4
  • Jiana Li
    • 2
  • Wei Qian
    • 2
    Email author
  • Huasheng Yu
    • 1
    Email author
  1. 1.Institute of Crop and Nuclear Technology UtilizationZhejiang Academy of Agricultural SciencesHangzhouChina
  2. 2.College of Agronomy and BiotechnologySouthwest UniversityChongqingChina
  3. 3.Queensland Alliance for Agriculture and Food InnovationUniversity of QueenslandSt LuciaAustralia
  4. 4.Norddeutsche Pflanzenzucht Hans-Georg Lembke KGHohenliethGermany

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