, 213:35 | Cite as

Mapping of brown planthopper [Nilaparvata lugens (Stål)] resistance gene (bph5) in rice (Oryza sativa L.)

  • Ram Deen
  • K. Ramesh
  • G. Padmavathi
  • B. C. Viraktamath
  • T. RamEmail author


Brown planthopper (BPH) has emerged as one of the most devastating pests of rice in several Asian countries. Cultivation of planthopper resistant varieties is an ecologically acceptable strategy. A population of 255 F2:3 families from the cross Taichung Native 1/ARC10550 was used to map BPH resistance with 106 polymorphic simple sequence repeat markers. The inheritance pattern of different traits suggested that the resistance in ARC10550 is controlled by quantitative traits instead of a single recessive gene. The quantitative trait loci (QTLs) for BPH resistance were analysed for nine phenotypic traits. Several of these phenotypic traits recorded high degree of positive or negative correlations between them, suggesting dependence or redundancy of the tests. QTL analysis revealed that five major loci were associated with resistance, one for damage score (qBphDs6) on chromosome 6, two for nymphal preference at 48 and 72 h (qBphNp(48h)-1 and qBphNp(72h)-12) on chromosome 1 and 12 and two for days to wilt (qBphDw(30)-3 and qBphDw(30)-8) on chromosome 3 and 8 explaining the phenotypic variance of 24.23, 8.69, 7.66, 4.55 and 10.48% respectively. These QTLs indicated the negative additive effects suggesting that the resistant alleles identified were from ARC10550 donor parent. These QTLs jointly explained 55.6% of the phenotypic variance for BPH resistance in this population.


Rice Brown planthopper Resistance Simple sequence repeats Quantitative trait loci 



We thank the Director, Indian Institute of Rice Research, Hyderabad for the facilities and encouragement. This work was partly supported by a grant from the Department of Biotechnology (DBT F.No. BT/AB/FG-2 (PH-II) Sep-2009), Government of India.


  1. Alagar M, Suresh S, Saravanan PA (2008) Feeding behaviour of Nilaparvata lugens (Stål.) on selected rice genotypes. Ann Plant Prot Sci 16(1):55–60Google Scholar
  2. Alam SN, Cohen MB (1998a) Detection and analysis of QTLs for resistance to the brown planthopper, Nilaparvata lugens, in a double-haploid rice population. Theor Appl Genet 97:1370–1379CrossRefGoogle Scholar
  3. Alam SN, Cohen MB (1998b) Durability of brown planthopper, Nilaparvata lugens, resistance in rice variety IR64 in greenhouse selection studies. Entomol Exp Appl 89:71–78CrossRefGoogle Scholar
  4. Cha YS, Ji H, Yun DW, Ahn BO, Lee MC, Suh SC, Lee CS, Ahn EK, Jeon YH, Jin ID, Sohn JK, Koh HJ, Eun MY (2008) Fine mapping of the rice Bph1 gene, which confers resistance to the brown planthopper [Nilaparvata lugens (Stål)], and development of STS markers for marker-assisted selection. Mol Cells 26:146–151PubMedGoogle Scholar
  5. Chen D, Wang GL, Ronald PC (1997) Location of the rice blast resistance locus Pi 5 (t) in Moroberekan by AFLP bulk segregant analysis. Rice Genet Newsl 14:95–97Google Scholar
  6. Cohen MB, Alam SN, Medina EB, Bernal CC (1997) Brown planthopper, Nilaparvata lugens, resistance in rice cultivar IR64: mechanism and role in successful N. lugens management in Central Luzon, Philippines. Entomol Exp Appl 85:221–229CrossRefGoogle Scholar
  7. Deen R, Ramesh K, Gautam SK, Rao YK, Lakshmi VJ, Viraktamath BC, Brar DS, Ram T (2010) Identification of new gene for BPH resistance introgressed from O. rufipogon. Rice Genet Newsl 25:70–72Google Scholar
  8. Du B, Zhang W, Liu B, Hu J, Wei Z, Shi Z, He R, Zhu L, Chen R, Han B, He G (2009) Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice. Proc Natl Acad Sci USA 106(52):22163–22168CrossRefPubMedPubMedCentralGoogle Scholar
  9. Duan CX, Su N, Cheng Z, Lei CL, Wang JL, Zhai HQ, Wan JM (2010) QTL analysis for the resistance to small brown planthopper, Laodelphax striatellus (Fallen) in rice using backcross inbred lines. Plant Breed 129:63–67CrossRefGoogle Scholar
  10. Fujita Daisuke, Kohli Ajay, Horgan Finbarr G (2013) Rice resistance to planthoppers and leafhoppers. Crit Rev Plant Sci 32(3):162–191. doi: 10.1080/07352689.2012.735986 CrossRefGoogle Scholar
  11. Gallagher KD, Kenmore PE, Sogawa K (1994) Judicial use of insecticides deter planthopper outbreaks and extend the life of resistant varieties in Southeast Asian rice. In: Denno RF, Perfect JT (eds) Planthoppers: their ecology and management. Chapman and Hall, New York, pp 599–614CrossRefGoogle Scholar
  12. Hao P, Liu C, Wang Y, Chen R, Tang M, Du B, Zhu L, He G (2008) Herbivore-induced callose deposition on the sieve plates of rice: an important mechanism of host resistance. Plant Physiol 146:1810–1820CrossRefPubMedPubMedCentralGoogle Scholar
  13. Heinrichs EA (1986) Perspectives and directions for the continued development of insect resistant rice varieties. Agric Ecosyst Environ 18(1):9–36CrossRefGoogle Scholar
  14. Heinrichs EA, Medrano FG, Rapusas HR (1985) Genetic evaluation for insect resistance in rice. International Rice Research Institute, Los Baños, pp 1–356Google Scholar
  15. Heninrichs EA, Mochida O (1984) From secondary to major pest status: the case of insecticide-induced rice brown planthopper, Nilaparvata lugens resurgence. Prot Ecol 7:201–218Google Scholar
  16. Hou LY, Ping YU, Qun XU, Yuan XP, Yu HY, Wang YP, Wang CH, Guo WA, Tang SX, Peng ST, Wei XH (2011) Genetic analysis and preliminary mapping of two recessive resistance genes to brown planthopper, Nilaparvata lugens Stål in rice. Rice Sci 18(3):238–242CrossRefGoogle Scholar
  17. Hu J, Xiao C, Cheng MX, Gao GJ, Zhang QL, He YQ (2015) A new finely mapped Oryza australiensis-derived QTL in rice confers resistance to brown planthopper. Gene 561(1):132–137. doi: 10.1016/j.gene.2015.02.026 CrossRefPubMedGoogle Scholar
  18. Huang Z, He G, Shu L, Li X, Zhang Q (2001) Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet 102:929–934CrossRefGoogle Scholar
  19. Standard evaluation system for rice (1996) Genetic Resources Centre, 4th edn. International Rice Research Institute, Los Baños, pp 29–30Google Scholar
  20. Jena KK, Jeung JU, Lee JH, Choi HC, Brar DS (2006) High resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t) and marker assisted selection for BPH resistance in rice (Oryza sativa L.). Theor Appl Genet 112:288–297CrossRefPubMedGoogle Scholar
  21. Kalode MB, Kasi PR, Viswanathan Seshu DV (1975) Standard test to characterize host plant resistance to brown planthopper in rice. Indian J Plant Prot 3(2):204–206Google Scholar
  22. Kar S, Verulkar SB, Verma S, Pali V (2011) Identification of QTLs for brown planthopper (Nilaparvata lugens Stål) resistance in RIL mapping population of rice (Oryza sativa L.). Plant Arch 11(2):687–692Google Scholar
  23. Kennedy GG, Gould F, Deponti OMB, Stinner RE (1987) Ecological, agricultural, genetic and commercial considerations in the deployment of insect-resistant germplasm. Environ Entomol 16:327–338CrossRefGoogle Scholar
  24. Khush GS, Karim ANMR, Angeles ER (1985) Genetics of resistance of rice cultivar ARC10550 to Bangladesh brown planthopper biotype. J Genet 64:121–125CrossRefGoogle Scholar
  25. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  26. Krishna TS, Sheshu DV, Kalode MB (1984) Inheritance of resistance to whitebacked planthopper in rice. Indian J Genet 44(2):329–335Google Scholar
  27. Li R, Li L, Wei S, Wei Y, Chen Y, Bai D, Yang L, Huang E, Lu W, Zhang X, Li X, Yang X, Wei Y (2006) The evaluation and utilization of new genes for brown planthopper resistance in common wild rice (Oryza rufipogon Griff.). Mol Plant Breed 4:365–371Google Scholar
  28. Liu Y, Su C, Jiang L, He J, Wu H, Peng C, Wan J (2009) The distribution and identification of brown planthopper resistance genes in rice. Hereditas 146(2):67–73CrossRefPubMedGoogle Scholar
  29. Medina EB, Bernal CC, Cohen MB (1996) Role of host plant resistance in successful control of brown planthopper in Central Luzon, Philippinenes. IRRN 21(2–3):53Google Scholar
  30. Murata K, Fujiwara M, Murai H, Takumi S, Mori N, Nakamura C (2001) Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of chromosome 12. Cereal Res Commun 29:245–250Google Scholar
  31. Myint KKM, Yasui H, Takagi M, Matsumura M (2009a) Virulence of long-term laboratory populations of the brown planthopper, Nilaparvata lugens (Stål), and whitebacked planthopper, Sogatella furcifera (Horvath; Homoptera: Delphacidae), on rice differential varieties. Appl Entomol Zool 44:149–153CrossRefGoogle Scholar
  32. Myint KKM, Matsumura M, Takagi M, Yasui H (2009b) Demographic parameters of long-term laboratory strains of the brown planthopper, Nilaparvata lugens Stål (Homoptera: Delphacidae) on resistance genes bph20(t) and Bph21(t) in rice. J Fac Agric Kyushu Univ 54:159–164Google Scholar
  33. Myint KKM, Fujita D, Matsumura M, Sonoda T, Yoshimura A, Yasui H (2012) Mapping and pyramiding of two major genes for resistance to the brown planthopper (Nilaparvata lugens Stål) in the rice cultivar ADR52. Theor Appl Genet 124:495–504CrossRefPubMedGoogle Scholar
  34. Normile D (2008) Agricultural research reinventing rice to feed the world. Science 321:330–333CrossRefPubMedGoogle Scholar
  35. Paguia P, Pathak MD, Heinrichs EA (1980) Honeydew excretion measurement techniques for determining differential feeding activity of biotypes of Nilaparvata lugens on rice varieties. J Econ Entomol 73:35–40CrossRefGoogle Scholar
  36. Panda N, Heinrichs EA (1983) Levels of tolerance and antibiosis in rice varieties having moderate resistance to the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae). Environ Entomol 12:1204–1214CrossRefGoogle Scholar
  37. Qiu YF, Guo JP, Jing SL, Zhu LL, He GC (2010) High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds. Theor Appl Genet 121:1601–1611CrossRefPubMedGoogle Scholar
  38. Qiu YF, Guo JP, Jing SL, Tang M, Zhu LL, He GC (2011) Identification of antibiosis and tolerance in rice varieties carrying brown planthopper resistance genes. Entomol Exp Appl 141:224–231CrossRefGoogle Scholar
  39. Qiu YF, Guo JP, Jing SL, Zhu LL, He GC (2012) Development and characterization of japonica rice lines carrying the brown planthopper resistance genes Bph12 and Bph6. Theor Appl Genet 124(3):485–494CrossRefPubMedGoogle Scholar
  40. Ram T, Deen R, Ramesh K, Gautam SK, Rao YK, Brar DS (2010) Identification of new genes for brown planthopper resistance in rice introgressed from O. glaberrima and O. minuta. Rice Genet Newsl 25:67–69Google Scholar
  41. Ren X, Wang X, Yuan H, Weng Q, Zhu L, He G (2004) Mapping quantitative trait loci and expressed sequence tags related to brown planthopper resistance in rice. Plant Breed 123:342–348CrossRefGoogle Scholar
  42. Renganayaki K, Fritz AK, Sadasivam S, Pammi S, Harrington S, McCouch SR, Kumar SM, Reddy AS (2000) Mapping and progress toward map-based cloning of brown planthopper biotype-4 resistance gene introgressed from Oryza officinalis into cultivated rice O. sativa. Crop Sci 42:2112–2117CrossRefGoogle Scholar
  43. Santhanalakshmi S, Saikumar S, Rao S, Sai Harini A, Khera P, Shashidhar HE, Kadirvel P (2010) Mapping genetic locus linked to brown planthopper resistance in rice (Oryza sativa L.). Int J Plant Breed Genet 4(1):13–22CrossRefGoogle Scholar
  44. Shi ZY, Ren X, Weng QM, Li XH, He CG (2003) Construction of a genomic library from a brown planthopper resistant rice line spanning the Qvb one locus. Plant Sci 165:879–885CrossRefGoogle Scholar
  45. Sogawa K (1982) The rice brown planthopper: feeding physiology and host plant interactions. Ann Rev Entomol 27:49–73CrossRefGoogle Scholar
  46. Soundararajan RP, Kadirvel P, Gunathilagaraj K, Maheswaran M (2004) Mapping of quantitative trait loci associated with resistance to brown planthopper in rice by means of a doubled haploid population. Crop Sci 44:2214–2220CrossRefGoogle Scholar
  47. Su CC, Cheng XN, Zhai HQ, Wan JM (2002) Detection and analysis of QTL for resistance to brown planthopper, Nilaparvata lugens (Stål), in rice (Oryza sativa L.), using backcross inbred lines. Acta Genet Sin 29:332–338PubMedGoogle Scholar
  48. Sun L, Su C, Wang C, Zai H, Wan J (2005) Mapping of a major resistance gene to brown planthopper in the rice cultivar Rathu Heenati. Breed Sci 55:391–396CrossRefGoogle Scholar
  49. Sun LH, Wang CM, Su CC, Liu YQ, Zhai HQ, Wan J (2006) Mapping and marker-assisted selection of a brown planthopper resistance gene bph2 in rice (Oryza sativa L.). Acta Genet Sin 33:717–723CrossRefPubMedGoogle Scholar
  50. Sun L, Liu Y, Jiang L, Su C, Wang G, Zhai H, Wan J (2007) Identification of quantitative trait loci associated with resistance to brown planthopper in the indica rice cultivar Col. 5 Thailand. Hereditas 144(2):48–52CrossRefPubMedGoogle Scholar
  51. Tamura Y, Hottori M, Yoshioka H, Yoshioka M, Takahashi A, Sentoku N, Yasui H (2014) Map-based cloning and characterization of a brown planthopper resistance gene Bph26 from Oryza sativa L. ssp. indica cultivar ADR52. Sci Rep 4:5872–5879PubMedGoogle Scholar
  52. Tuyen L, Liu Y, Jiang L, Wang BX, Wang Q, Than TTH, Wan JM (2012) Identification of quantitative trait loci associated with small brown planthopper, Laodelphax striatellus (Fallen) resistance in rice (Oryza sativa L.). Hereditas 149:16–23CrossRefGoogle Scholar
  53. Van Ooijen JW (2006) JoinMap 4.0 software for the calculation of genetic linkage maps in experimental populations. Kyzama B V, WageningenGoogle Scholar
  54. Voorrips R (2002) Map Chart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78CrossRefPubMedGoogle Scholar
  55. Wang S, Basten CJ, Zeng ZB (2007) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh. User manual, pp 1–87.
  56. Wang Q, Liu Y, Hu J, Zhang Y, Xie K, Wang B, Tuyen LQ, Song Z, Wu H, Liu Y, Jiang L, Liu S, Cheng X, Wang C, Zhai H, Wan J (2013) Detection of quantitative trait loci (QTLs) for resistances to small brown planthopper and rice stripe virus in rice using recombinant inbred lines. Int J Mol Sci 14:8406–8421. doi: 10.3390/ijms14048406 CrossRefPubMedPubMedCentralGoogle Scholar
  57. Wu H, Liu Y, He J, Liu Y, Jiang L, Liu L, Wang C, Cheng X, Wan J (2014) Fine mapping of brown planthopper (Nilaparvata lugens Stål) resis-tancegene Bph28(t) in rice (Oryza sativa L.). Mol Breed 33:909–918CrossRefGoogle Scholar
  58. Xu XF, Mei HW, Luo LJ, Cheng XN, Li ZK (2002) RFLP facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens). Theor Appl Genet 104:248–253CrossRefPubMedGoogle Scholar
  59. Yang L, Li RB, Li YR, Huang FK, Chen YZ, Huang SS, Huang LF, Liu C, Ma ZF, Huang DH, Jiang JJ (2012) Genetic mapping of bph20(t) and bph21(t) loci conferring brown planthopper resistance to Nilaparvata lugens Stål in rice (Oryza sativa L.). Euphytica 183:161–171CrossRefGoogle Scholar
  60. Zeng Z (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468PubMedPubMedCentralGoogle Scholar
  61. Zheng KL, Subudhi PK, Domingo J, Magpantay G (1995) Rapid DNA isolation for marker assisted selection in rice breeding. Rice Genet Newsl 12:255–258Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Crop Improvement SectionIndian Institute of Rice ResearchHyderabadIndia

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