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Development of chromosome segment substitution lines (CSSLs) of Oryza longistaminata A. Chev. & Röhr in the background of the elite japonica rice cultivar, Taichung 65 and their evaluation for yield traits

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Abstract

Oryza longistaminata (AA genome) is a wild rice species that is phenotypically inferior to cultivated rice but possesses useful alleles that can be used to improve agronomically important traits. Interspecific hybrids that are derived from cultivated rice and wild rice species with AA genome are important contributors of genetic diversity in rice. To illustrate the potential of wild rice relatives as a source of novel alleles for rice improvement, a total of 40 chromosome segment substitution lines (CSSLs) of O. longistaminata in the background of the elite japonica cultivar Taichung 65 were developed and evaluated for yield and various yield-related traits. A number of CSSLs carrying putative quantitative trait loci (QTLs) controlling different yield-related traits were identified during both dry and wet seasons. In particular, 10 major putative QTLs controlling early heading date, plant height, tiller number, panicle length, number of primary branches per panicle, grain number per panicle, grain width, and grain thickness were identified. Interestingly, one of the CSSL lines, LTSL26, with major putative QTLs on chromosomes 1 and 8 that increase grain number per panicle, showed pleiotropic effects on other traits such as plant height, days to flowering, tiller number, number of branches per panicle, and grain length. These results suggest that O. longistaminata is a good source of new alleles that can be used to improve yield-related traits in cultivated rice varieties.

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Acknowledgments

Our sincere thanks go to the members of the Novel Gene Resources Laboratory (previously Wide Hybridization Laboratory), particularly to Mr. Eleazar “Boyet” Manalaysay, who helped in the production of LTSLs and in the evaluation of the materials. This research is supported by the Japan Society for the Promotion of Science (JSPS) Ronpaku Program in collaboration with the International Rice Research Institute, Philippines; Nagoya University, Japan, and the Science and Technology Research Program for Agriculture, Forestry, Fisheries and Food Industry; and is partially supported by JST, CREST.

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Authors and Affiliations

  1. Novel Gene Resources Laboratory, Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

    Joie M. Ramos, Rosalyn B. Angeles-Shim, Junghyun Shim & Kshirod K. Jena

  2. Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi, 464-8601, Japan

    Joie M. Ramos, Tomoyuki Furuta, Kanako Uehara, Niwa Chihiro, Rosalyn B. Angeles-Shim & Motoyuki Ashikari

  3. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India

    Darshan S. Brar

  4. CREST, Japan Science and Technology Agency, Tokyo, Japan

    Motoyuki Ashikari

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  1. Joie M. Ramos
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  2. Tomoyuki Furuta
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  3. Kanako Uehara
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  4. Niwa Chihiro
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  5. Rosalyn B. Angeles-Shim
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  6. Junghyun Shim
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  8. Motoyuki Ashikari
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  9. Kshirod K. Jena
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Correspondence to Kshirod K. Jena.

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Ramos, J.M., Furuta, T., Uehara, K. et al. Development of chromosome segment substitution lines (CSSLs) of Oryza longistaminata A. Chev. & Röhr in the background of the elite japonica rice cultivar, Taichung 65 and their evaluation for yield traits. Euphytica 210, 151–163 (2016). https://doi.org/10.1007/s10681-016-1685-3

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  • DOI: https://doi.org/10.1007/s10681-016-1685-3

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