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Euphytica

, Volume 209, Issue 3, pp 693–707 | Cite as

Resistance to yellow spot in wheat grown under accelerated growth conditions

  • Eric DinglasanEmail author
  • Ian D. Godwin
  • Miranda Y. Mortlock
  • Lee T. Hickey
Article

Abstract

Yellow spot, also known as tan spot (YS), is a serious fungal foliar disease of wheat worldwide. The introduction of resistance to YS in wheat cultivars offers the most durable, economic, and environmentally safe management strategy. Adult plant resistance (APR) is preferred over seedling resistance to control other diseases in wheat and has the potential to offer non-race specific resistance to YS. The search for APR currently relies on screening vast numbers of wheat genotypes in the field, where expression is affected by environmental factors. We report a rapid phenotyping method for APR to YS that combines use of constant light and controlled temperatures to achieve accelerated growth conditions (AGC). A panel comprising 20 spring wheat genotypes was evaluated in four separate experiments: (1) seedling stage under regular greenhouse conditions; (2) adult-plant (AP) stage under AGC; (3) integrated seedling and AP disease assessment; and (4) AP stage in the field. Phenotypes from all AP experiments conducted under controlled and field conditions correlated well (r = 0.71–0.84), but correlations between AP and seedling phenotypes were weaker (r = 0.30–0.62). Moderate to high levels of APR were displayed by some genotypes (e.g. CIMMYT line ‘ZWW10-50’) that were equivalent to levels attained by seedling resistant cultivar ‘Leichhardt’. An integrated cycle requires only seven weeks to complete, and provides a useful tool for breeders and pathologists to efficiently phenotype APR to YS under controlled conditions.

Keywords

Adult-plant resistance Disease screening methods Tan spot Wheat breeding 

Notes

Acknowledgments

This research was partially supported by the University of Queensland through an international PhD scholarship. We acknowledge Mr. Greg Platz (Department of Agriculture and Fisheries) for valuable advice relating to the inoculation and field-based inoculum increase techniques reported in this study and PhD student Mr. Adnan Riaz for assistance with glasshouse experiments.

References

  1. Ali S, Adhikari T, Barbara S, Cole H (2006) Pyrenophora tritici-repentis (tan spot) races in Australia. Phytopathology 96:S4CrossRefGoogle Scholar
  2. Anderson JA, Effertz RJ, Faris JD, Francl LJ, Meinhardt SW, Gill BS (1999) Genetic analysis of sensitivity to a Pyrenophora tritici-repentis necrosis-inducing toxin in durum and common wheat. Phytopathology 89:293–297CrossRefPubMedGoogle Scholar
  3. Antoni EA, Rybak K, Tucker MP, Hane JK, Solomon PS, Drenth A, Shankar M, Oliver RP (2010) Ubiquity of ToxA and absence of ToxB in Australian populations of Pyrenophora tritici-repentis. Aust Plant Pathol 39:63–68CrossRefGoogle Scholar
  4. Bostock RM, Pye MF, Roubtsova TV (2014) Predisposition in plant disease: exploiting the nexus in abiotic and biotic stress perception and response. Annu Rev Phytopathol 52:517–549CrossRefPubMedGoogle Scholar
  5. Cheong J, Wallwork H, Williams KJ (2004) Identification of a major QTL for yellow leaf spot resistance in the wheat varieties Brookton and Cranbook. Aust J Agric Res 55:315–319CrossRefGoogle Scholar
  6. Chu CG, Friesen TL, Xu SS, Faris JD (2008) Identification of novel tan spot resistance loci beyond the known host-selective toxin insensitivity genes in wheat. Theor Appl Genet 117:873–881CrossRefPubMedGoogle Scholar
  7. Chu CG, Chao S, Friesen TL, Faris JD, Zhong S, Xu SS (2010) Identification of novel tan spot resistance QTLs using an SSR-based linkage map of tetraploid wheat. Mol Breed 25:327–338CrossRefGoogle Scholar
  8. Ciuffetti LM, Tuori RP (1999) Advances in the characterization of the Pyrenophora tritici-repentis–wheat interaction. Phytopathology 89:444–449CrossRefPubMedGoogle Scholar
  9. Ciuffetti LM, Manning VA, Pandelova I, Betts MF, Martinez JP (2010) Host-selective toxins, Ptr ToxA and Ptr ToxB, as necrotrophic effectors in the Pyrenophora tritici-repentis–wheat interaction. New Phytol 187:911–919CrossRefPubMedGoogle Scholar
  10. Cox DJ, Hosford RM (1987) Resistant winter wheats compared at differing growth stages and leaf positions for tan spot severity. Plant Dis 71:883–886CrossRefGoogle Scholar
  11. da Luz WC, Bergstrom GC (1987) Interaction between Cochliobolus sativus and Pyrenophora tritici-repentis on wheat leaves. Pythopathology 77:1355–1360CrossRefGoogle Scholar
  12. Dumasalová V, Svobodová L, Hanzalová A (2012) Differentially expressed gene transcripts in wheat and barley leaves upon leaf spot infection. Czech J Genet Plant Breed 48(3):108–119Google Scholar
  13. Effertz RJ, Anderson JA, Francl LJ (2001) Restriction fragment length polymorphism mapping of resistance to two races of Pyrenophora tritici-repentis in adult and seedling wheat. Phytopathology 91:572–578CrossRefPubMedGoogle Scholar
  14. Effertz RJ, Meinhardt SW, Anderson JA, Jordahl JG, Francl LJ (2002) Identification of a chlorosis-inducing toxin from Pyrenophora tritici-repentis and the chromosomal location of an insensitivity locus in wheat. Phytopathology 92:527–533CrossRefPubMedGoogle Scholar
  15. Evans CK, Hunger RM, Siegeris WC (1999) Comparison of greenhouse and field testing to identify wheat resistant to tan spot. Plant Dis 83:269–273CrossRefGoogle Scholar
  16. Faris JD, Friesen TL (2005) Identification of quantitative trait loci for race-nonspecific resistance to tan spot in wheat. Theor Appl Genet 111:386–392CrossRefPubMedGoogle Scholar
  17. Faris JD, Anderson JA, Francl LJ, Jordahl JG (1997) RFLP mapping of resistance to chlorosis induction by Pyrenophora tritici-repentis in wheat. Theor Appl Genet 94:98–103CrossRefPubMedGoogle Scholar
  18. Faris JD, Zhang Z, Lu H, Lu S, Reddy L, Cloutier S, Fellers JP, Meinhardt SW, Rasmussen JB, Xu SS, Oliver RP, Simons KJ, Friesen TL (2010) A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens. Proc Nat Acad Sci USA 107:13544–13549CrossRefPubMedPubMedCentralGoogle Scholar
  19. Faris JD, Abeysekara NS, McClean PE, Xu SS, Friesen TL (2012) Tan spot susceptibility governed by the Tsn1 locus and race nonspecific resistance quantitative trait loci in a population derived from the wheat lines Salamouni and Katepwa. Mol Breed 30:1669–1678CrossRefGoogle Scholar
  20. Faris JD, Liu Z, Xu SS (2013) Genetics of tan spot resistance in wheat. Theor Appl Genet 126:2197–2217CrossRefPubMedGoogle Scholar
  21. Fernandez MR, Clarke JM, DePauw RM (1994) Response of durum wheat kernels and leaves at different growth stages to Pyrenophora tritici-repentis. Plant Dis 78:597–600CrossRefGoogle Scholar
  22. GRDC–NVT (2015) Grains Research and Development Corporation—National Variety Trial. http://www.nvtonline.com.au/
  23. Gurung S, Mamidi S, Bonman JM, Jackson EW, del Río LE, Acevedo M, Mergoum M, Adhikari TB (2011) Identification of novel genomic regions associated with resistance to Pyrenophora tritici-repentis races 1 and 5 in spring wheat landraces using association analysis. Theor Appl Genet 123:1029–1041CrossRefPubMedGoogle Scholar
  24. Hickey LT, Wilkinson PM, Knight CR, Godwin ID, Kravchuk OY, Aitken EB, Bansal UK, Bariana HS, Delacy IH, Dieters MJ (2012) Rapid phenotyping for adult-plant resistance to stripe rust in wheat. Plant Breed 131:54–61CrossRefGoogle Scholar
  25. Hollaway G (2014) The yellow spot of wheat. The State of Victoria Department of Environment and Primary Industries, Melbourne, AustraliaGoogle Scholar
  26. Hosford RM, Jordahl JG Jr, Hammond JJ (1990) Effect of wheat genotype, leaf position, growth stage, fungal isolate, and wet period on tan spot lesions. Plant Dis 74:385–390CrossRefGoogle Scholar
  27. Hua J (2013) Modulation of plant immunity by light, circadian rhythm, and temperature. Curr Opin Plant Biol 16:406–413CrossRefPubMedGoogle Scholar
  28. Istifadah N, McGee PA (2006) Endophytic Chaetomium globosum reduces development of tan spot in wheat caused by Pyrenophora tritici-repentis. Aust Plant Pathol 35:411–418CrossRefGoogle Scholar
  29. Jackson SD (2009) Plant responses to photoperiod. New Phytol 181:517–531CrossRefPubMedGoogle Scholar
  30. Jørgensen LN, Olsen LV (2007) Control of tan spot (Drechslera tritici-repentis) using cultivar resistance, tillage methods and fungicides. Crop Prot 26:1606–1616CrossRefGoogle Scholar
  31. Lamari L, Bernier CC (1989) Evaluation of wheat lines and cultivars to tan spot (Pyrenophora tritici-repentis) based on lesion type. Can J Plant Pathol 11:49–56CrossRefGoogle Scholar
  32. Li HB, Yan W, Liu GR, Wen SM, Liu CJ (2011) Identification and validation of quantitative trait loci conferring tan spot resistance in the bread wheat variety Ernie. Theor Appl Genet 122:395–403CrossRefPubMedGoogle Scholar
  33. Liu Z, Friesen TL, Ling H, Meinhardt SW, Oliver RP, Rasmussen JB, Faris JD (2006) The Tsn-1-ToxA interaction in the wheat-Stagonospora nodorum pathosystem parallels that of wheat-tan spot system. Genome 49:1265–1273CrossRefPubMedGoogle Scholar
  34. Lo Iacono G, van den Bosch F, Gilligan CA (2013) Durable resistance to crop pathogens: an epidemiological framework to predict risk under uncertainty. PLoS Comput Biol 9(1):e1002870CrossRefPubMedPubMedCentralGoogle Scholar
  35. Manning VA, Ciuffetti LM (2005) Localization of Ptr ToxA produced by Pyrenophora tritici-repentis reveals protein import into wheat mesophyll cells. Plant Cell 17:3203–3212CrossRefPubMedPubMedCentralGoogle Scholar
  36. Martinez JP, Oesch NW, Ciuffetti LM (2004) Characterization of the multiple-copy host-selective toxin gene, ToxB, in pathogenic and non-pathogenic isolates of Pyrenophora tritici-repentis. Mol Plant-Microbe Interact 17:467–474CrossRefPubMedGoogle Scholar
  37. Michelmore RW (2003) The impact zone: genomics and breeding for durable disease resistance. Curr Opin Plant Biol 6:397–404CrossRefPubMedGoogle Scholar
  38. Moreno MV, Stenglein SA, Perelló AE (2012): Pyrenophora tritici-repentis, causal agent of tan spot: a review of intraspecific genetic diversity. In: Mahmut Caliskan (ed) The molecular basis of plant genetic diversity, ISBN: 978-953-51-0157-4 In Tech, Available from: http://www.intechopen.com/books/themolecular-basis-of-plant-genetic-diversity/pyrenophora-tritici-repentis-causal-agent-of-tan-spot-areview-ofintraspecific-genetic-diversity
  39. Morris JF, Carver BF, Hunger RM, Klatt AR (2010) Greenhouse assessment of seedling reaction to tan spot in synthetic hexaploid wheat. Crop Sci 50:952–959CrossRefGoogle Scholar
  40. Murray GM, Brennan JP (2009) Estimating disease losses to the Australian wheat industry. Aust Plant Pathol 38:558–570CrossRefGoogle Scholar
  41. Oliver RP, Lord M, Rybak K, Faris JD, Solomon SP (2008) Emergence of tan spot disease caused by toxigenic Pyrenophora tritici-repentis in Australia is not associated with increased deployment of toxin sensitive cultivars. Phytopathology 98:488–491CrossRefPubMedGoogle Scholar
  42. Oliver RP, Rybak K, Solomon PS, Ferguson-Hunt M (2009) Prevalence of ToxA-sensitive alleles of the wheat gene Tsn1 in Australian and Chinese wheat cultivars. Crop Pasture Sci 60:348–352CrossRefGoogle Scholar
  43. Orolaza NP, Lamari L, Ballance GM (1995) Evidence of a host-specific chlorosis toxin from Pyrenophora tritici-repentis, the causal agent of tan spot of wheat. Phytopathology 85:1282–1287CrossRefGoogle Scholar
  44. Pandelova I, Figueroa M, Wilhelm LJ, Manning VA, Mankaney AN (2012) Host-selective toxins of Pyrenophora tritici-repentis induce common responses associated with host susceptibility. PLoS ONE 7(7):e40240CrossRefPubMedPubMedCentralGoogle Scholar
  45. Pavan S, Jacobsen E, Visser RGF, Bai Y (2010) Loss of susceptibility as a novel breeding strategy for durable and broad-spectrum resistance. Mol Breed 25:1–12CrossRefPubMedPubMedCentralGoogle Scholar
  46. Perello A, Moreno V, Simón MR, Sisterna M (2003) Tan spot of wheat (Triticum aestivum L.) infection at different stages of crop development and inoculum type. Crop Prot 22:157–169CrossRefGoogle Scholar
  47. Platt HW, Morrall RAA, Gruen HE (1977) The effects of substrate, temperature, and photoperiod on conidiation of Pyrenophora tritici-repentis. Can J Bot 55:254–259CrossRefGoogle Scholar
  48. Poland JA, Balint-Kurti PJ, Wisser RJ, Pratt RC, Nelson RJ (2009) Shades of gray: the world of quantitative disease resistance. Trends Plant Sci 14:21–29CrossRefPubMedGoogle Scholar
  49. R Core Team (2014) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL http://www.R-project.org/
  50. Raymond PJ, Bockus WW, Norman BL (1985) Tan spot of winter wheat: procedures to determine host response. Phytopathology 75:686–690CrossRefGoogle Scholar
  51. Rees RG, Platz GJ (1979) The occurrence and control of yellow spot of wheat in north-eastern Australia. Aust J Exp Agric Anim Husb 19:369–372CrossRefGoogle Scholar
  52. Rees RG, Platz GJ (1989) Effectiveness of incomplete resistance to Pyrenophora tritici-repentis in wheat. Aust J Agric Res 40:43–48CrossRefGoogle Scholar
  53. Rees RG, Platz GJ (1990) Sources of resistance to Pyrenophora tritici-repentis in bread wheats. Euphytica 45:56–69Google Scholar
  54. Rees RG, Platz GJ, Mayer RJ (1982) Yield losses in wheat from yellow spot: comparison of estimates derived from single tillers and plots. Aust J Agric Res 33:899–908CrossRefGoogle Scholar
  55. Rees RG, Platz GJ, Mayer RJ (1987) Susceptibility of Australian wheats to Pyrenophora tritici-repentis. Aust J Agric Res 39:141–151CrossRefGoogle Scholar
  56. Riede CR, Francl LJ, Anderson JA, Jordahl JG, Meinhardt SW (1996) Additional sources of resistance to tan spot of wheat. Crop Sci 36:771–777CrossRefGoogle Scholar
  57. Ronis A, Semaškienė R (2006) Development of tan spot (Pyrenophora tritici-repentis) in winter wheat under field conditions. Agron Res 4:331–334Google Scholar
  58. Schoeneweiss DF (1975) Predisposition, stress, and plant disease. Annu Rev Phytopathol 13:193–211CrossRefGoogle Scholar
  59. Shackley B, Zaicou-Kunesch C, Dhammu H, Shankar M, Amjad M, Young K (2014) Wheat variety guide for WA 2014, Bulletin 4857. Western Australia Agriculture Authority, Perth, AustraliaGoogle Scholar
  60. Shankar M, Foster D, Jorgensen D (2013) A novel reliable method of field screening for yellow spot resistance. Crop Updates 2013, Grain Industry Association of Western Australia, Perth, AustraliaGoogle Scholar
  61. Singh PK, Mergoum M, Gonzalez-Hernandez JL, Ali S, Adhikari TB, Kianian SF, Elias EM, Hughes GR (2008) Genetics and molecular mapping of resistance to necrosis inducing race 5 of Pyrenophora tritici-repentis in tetraploid wheat. Mol Breed 21:293–304CrossRefGoogle Scholar
  62. Singh PK, Duveiller E, Singh RP (2011) Evaluation of CIMMYT germplasm for resistance to leaf spotting diseases of wheat. Czech J Genet Plant Breed 47:S102–S108Google Scholar
  63. Singh PK, Duveiller E, Singh RP (2012) Resistance breeding for tan Spot (Pyrenophora tritici-repentis) of wheat. In: Sharma I (ed) Disease resistance in wheat. CAB International, Wallingford, pp 136–150CrossRefGoogle Scholar
  64. Sysoeva MI, Markovskaya EF, Shibaeva TG (2010) Plants under continuous light: a review. Plant Stress 4(1):5–17Google Scholar
  65. Tadesse W, Schmolke M, Hsam SLK, Mohler V, Wenzel G, Zeller FJ (2007) Molecular mapping of resistance genes to tan spot [Pyrenophora tritici-repentis race 1] in synthetic wheat lines. Theor Appl Genet 114:855–862CrossRefPubMedGoogle Scholar
  66. Tadesse W, Reents HJ, Hsam SLK, Zeller FJ (2011) Relationship of seedling and adult plant resistance and evaluation of wheat germplasm against tan spot (Pyrenophora tritici-repentis). Genet Resour Crop Evol 58:339–346CrossRefGoogle Scholar
  67. Tomas A, Feng GH, Reeck GR, Bockus WW, Leach JE (1990) Purification of a cultivar-specific toxin from Pyrenophora tritici-repentis, causal agent of tan spot of wheat. Mol Plant-Microbe Interact 3:221–224CrossRefGoogle Scholar
  68. Velez-Ramirez AI, van Ieperen W, Vreugdenhil D, Millenaar FF (2011) Plants under continuous light. Trends Plant Sci 16:310–318CrossRefPubMedGoogle Scholar
  69. Wheat Varieties (2015) Queensland. Grains Research and Development Corporation (GRDC) and Department of Employment, Economic Development and Innovation (DEEDI). www.nvtonline.com.au
  70. Xu SS, Friessen TL, Mujeeb-Kazi A (2004) Seedling resistance to tan spot and Stagonospora nodorum blotch in synthetic hexaploid wheats. Crop Sci 44:2238–2245CrossRefGoogle Scholar
  71. Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421CrossRefGoogle Scholar
  72. Zhang HF, Francl LJ, Jordahl JG, Meinhardt SW (1997) Structural and physical properties of a necrosis-inducing toxin from Pyrenophora tritici-repentis. Phytopathology 87:154–160CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandSt LuciaAustralia
  2. 2.School of Agriculture and Food SciencesThe University of QueenslandSt LuciaAustralia

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