Ectopic expression of the Vigna eylindrica ferritin gene enhanced heat tolerance in transgenic wheat (Triticum aestivum L.)
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The Ferritins are the iron storage proteins which regulate intracellular iron content via the absorption and release of iron, and related to plant oxidative stress and pathogens. However, the possible role of FER in heat stress responses remains unknown. In this study, the black rice (Vigna eylindrica) ferritin gene, VeFER, was cloned and integrated into the genome of wheat cultivar ZY9507 via biolistic transformation. Expression analysis of T3 transgenic plants subjected to heat treatment showed significantly increased VeFER expression in the transgenic lines as compared to the untransformed plants. The MDA content of the transgenic lines was significantly lower than that in the cultivar. Further, the transgenic lines had similar relative electrical conductivities to the heat resistant variety TAM107, but were significantly lower than the untransformed control cultivar and the heat-sensitive variety CS. These results indicate that the VeFER gene plays a crucial role in improving the heat tolerance of transgenic wheat plants, likely by promoting the thermal stability of cell membranes.
KeywordsWheat (Triticum aestivum L.) Ferritin Heat tolerance
Vigna eylindrica ferritin gene
Polymerase chain reaction
quantitative real time PCR
Reverse transcription PCR
This work was financially supported by the National Transgenic Program of China (2011ZX08002001) and National Basic Research Program of China (973 Program) (2009CB118300).
Compliance with Ethical Standards
Conflict of interest
The authors declared that this study “Ectopic Expression of the Vigna eylindrica FER Gene Enhanced Heat Tolerance in Transgenic Wheat (Triticum aestivum L.)” have not been submitted for publication elsewhere. It is also declared that all authors have contributed significantly to the work and that all authors are in agreement with the content of the manuscript. The authors do not have any possible conflict of interest.
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