Mapping of Pi, a gene conferring pink leaf in ornamental kale (Brassica oleracea L. var. acephala DC)
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We previously found that pink leaf is conferred by a single semi-dominant gene in ornamental kale. To map this gene, we constructed an F2 segregating population containing 184 individuals by crossing W02–7, a white-leaved inbred line, with P02–9, a pink-leaved inbred line. We screened 297 simple sequence repeat (SSR) and 437 sequence-related amplified polymorphism (SRAP) markers using bulked segregant analysis. We identified one SSR and seven SRAP markers that linked tightly to the pink-leaf (Pi) gene. To obtain more specific genomic markers, we converted six SRAPs to sequence-characterized amplified region (SCAR) markers. We constructed a genetic linkage map of the Pi locus using one SSR and five SCAR markers, spanning a total interval of 15.0 cM. One SSR marker, Ni2C12, and one co-dominant SCAR marker, Boac04, flanked Pi on either side at distances of 0.6 and 2.4 cM, respectively. Based on the reference genome sequence of Brassica oleracea, we positioned Pi on the top of chromosome C3. We also detected that pink hypocotyl co-segregated with pink inner leaf. These markers provide the basis for fine-scale mapping and cloning of Pi, and may be used for marker-assisted selection in ornamental kale breeding programs.
KeywordsBrassica oleracea L.var. acephala DC Pink leaf Genetic mapping SSR SCAR
We thank Professor Zhongyun Piao (College of Horticulture, Shenyang Agricultural University) for his kindly help in database analysis. This study was funded by National Natural Science Foundation of China (# 31101566).
P Zhu developed molecular markers, carried out sequence analysis, and wrote draft manuscript. M Cheng and Y Xiong carried out the phenotyping and genotyping steps, developed SCAR and morphological markers, and selected recombinants. X Feng and C Liu developed SSR markers and constructed genetic map. Y Kang developed SRAP and SCAR markers. All authors have read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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