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Euphytica

, Volume 207, Issue 2, pp 245–254 | Cite as

Identification of QTLs controlling low-temperature germination of the East European rice (Oryza sativa L.) variety Maratteli

  • Tomohiro Satoh
  • Kouichi Tezuka
  • Tomohiko Kawamoto
  • Shinichi Matsumoto
  • Namiko Satoh-Nagasawa
  • Kenji Ueda
  • Kenji Sakurai
  • Akio Watanabe
  • Hidekazu Takahashi
  • Hiromori Akagi
Article

Abstract

Germinability under low temperature is one of the most important traits in seedling establishment in direct-sowing culture of rice. The objective of this study was the identification of genes responsible for higher and faster germination under low temperature, with the aim of breeding new rice varieties for direct-sowing culture. We identified four quantitative trait loci (QTLs) responsible for germination properties under low temperature in an East European rice variety, Maratteli. Maratteli-derived alleles at qLTG3-1 and qLTG3-2 on chromosome 3 and qLTG11-1 on chromosome 11 increased germination rate under low temperature. In contrast, the Maratteli allele at qLTG1-1 on chromosome 1 reduced the rate. Near-isogenic lines (NILs) with the Akitakomachi genetic background harboring qLTG3-1 and qLTG11-1 alleles from Maratteli accelerated germination and a NIL with qLTG1-1 delayed germination under low temperature, resulting in enhanced or suppressed germination rates, respectively. These QTLs altered the germination profile of Akitakomachi. Hence, Maratteli is a better source of genes for the improvement of germination under low temperature. We conclude that the low temperature germinability of various Japanese varieties inferior in this character can be improved by marker-assisted selection without modification of other superior traits. These NILs may also contribute to uncovering the molecular mechanisms of cold tolerance at the germination stage.

Keywords

Rice (Oryza sativa L.) Germinability at low temperature Direct-sowing QTLs NIL Marker-assisted selection 

Notes

Acknowledgments

We thank Dr. Hisanori Ohta of National Agriculture and Food Research Organization, Agricultural Research Center, for providing seeds of Maratteli rice. This research was supported by a grant from Akita Prefectural University in the industry-academia joint research program.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Tomohiro Satoh
    • 1
  • Kouichi Tezuka
    • 1
  • Tomohiko Kawamoto
    • 2
  • Shinichi Matsumoto
    • 2
  • Namiko Satoh-Nagasawa
    • 3
  • Kenji Ueda
    • 3
  • Kenji Sakurai
    • 3
  • Akio Watanabe
    • 3
  • Hidekazu Takahashi
    • 3
  • Hiromori Akagi
    • 3
  1. 1.Graduate School of Bioresource SciencesAkita Prefectural UniversityShimoshinjyo-NakanoJapan
  2. 2.Akita Agricultural Experiment StationYuwaJapan
  3. 3.Faculty of Bioresource SciencesAkita Prefectural UniversityShimoshinjyo-NakanoJapan

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