, Volume 206, Issue 1, pp 203–223 | Cite as

Integrative detection and verification of QTL for plant traits in two connected RIL populations of high-oil maize

  • G. H. Yang
  • Y. B. Dong
  • Y. L. Li
  • Q. L. Wang
  • Q. L. Shi
  • Q. Zhou


Plant traits play an important role in determining plant density and final grain yield. In this study, two related RIL populations developed from two crosses between one high-oil maize inbred and two normal dent maize inbreds were evaluated for 13 plant traits under four environments. QTL were detected within population and in joint-population analysis, and compared with the result obtained in the two F2:3 generations. Our main objective was to find identical QTL and key genetic regions valuable in further research. Totally, 318 single-population QTL, 142 pairs of digenic epistasis and 412 joint-population QTL were detected. Joint-population analysis could detect much more QTL and increase the accuracy of QTL localization. Consistent QTL across generations, environments and analysis methods were observed for five traits at four bins. QTL for plant height and ear height at bin 3.05, and for leaf area at bin 6.03–6.04 had the highest consistency across most situations. These QTL with high consistency were worthy to be put into marker-assisted selection in trait improvement and to construct near isogenic lines in further research. Maize breeding could be improved by integrating marker assisted selection and phenotypic selection.


High-oil maize Plant traits QTL Single population analysis Joint population analysis QTL consistency 



Composite interval mapping


Ear height


Illinois high oil


Illinois low oil


Joint inclusive composite interval mapping


Leaf angle


Leaf area


Leaf length


Number of leaves above the top ear


Logarithm of odds


Leaf orientation value


Leaf width


Marker-assisted selection


Multiple interval mapping


Nested association mapping


Near isogenic lines


Plant height


Quantitative trait locus/loci


Stalk diameter


Simple sequence repeat


Number of tassel branches


Top height


The ratio of top height to plant height


Tassel length

Supplementary material

10681_2015_1502_MOESM1_ESM.doc (480 kb)
Supplementary material 1 (DOC 480 kb)
10681_2015_1502_MOESM2_ESM.doc (208 kb)
Supplementary material 2 (DOC 208 kb)
10681_2015_1502_MOESM3_ESM.doc (706 kb)
Supplementary material 3 (DOC 706 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • G. H. Yang
    • 1
  • Y. B. Dong
    • 1
  • Y. L. Li
    • 1
  • Q. L. Wang
    • 1
  • Q. L. Shi
    • 1
  • Q. Zhou
    • 1
  1. 1.Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, College of AgricultureHenan Agricultural UniversityZhengzhouChina

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