Euphytica

, Volume 206, Issue 1, pp 203–223 | Cite as

Integrative detection and verification of QTL for plant traits in two connected RIL populations of high-oil maize

  • G. H. Yang
  • Y. B. Dong
  • Y. L. Li
  • Q. L. Wang
  • Q. L. Shi
  • Q. Zhou
Article

Abstract

Plant traits play an important role in determining plant density and final grain yield. In this study, two related RIL populations developed from two crosses between one high-oil maize inbred and two normal dent maize inbreds were evaluated for 13 plant traits under four environments. QTL were detected within population and in joint-population analysis, and compared with the result obtained in the two F2:3 generations. Our main objective was to find identical QTL and key genetic regions valuable in further research. Totally, 318 single-population QTL, 142 pairs of digenic epistasis and 412 joint-population QTL were detected. Joint-population analysis could detect much more QTL and increase the accuracy of QTL localization. Consistent QTL across generations, environments and analysis methods were observed for five traits at four bins. QTL for plant height and ear height at bin 3.05, and for leaf area at bin 6.03–6.04 had the highest consistency across most situations. These QTL with high consistency were worthy to be put into marker-assisted selection in trait improvement and to construct near isogenic lines in further research. Maize breeding could be improved by integrating marker assisted selection and phenotypic selection.

Keywords

High-oil maize Plant traits QTL Single population analysis Joint population analysis QTL consistency 

Abbreviations

CIM

Composite interval mapping

EH

Ear height

IHO

Illinois high oil

ILO

Illinois low oil

JICIM

Joint inclusive composite interval mapping

LA

Leaf angle

LAA

Leaf area

LL

Leaf length

LNE

Number of leaves above the top ear

LOD

Logarithm of odds

LOV

Leaf orientation value

LW

Leaf width

MAS

Marker-assisted selection

MIM

Multiple interval mapping

NAM

Nested association mapping

NILs

Near isogenic lines

PH

Plant height

QTL

Quantitative trait locus/loci

SD

Stalk diameter

SSR

Simple sequence repeat

TB

Number of tassel branches

TH

Top height

THPH

The ratio of top height to plant height

TL

Tassel length

Supplementary material

10681_2015_1502_MOESM1_ESM.doc (480 kb)
Supplementary material 1 (DOC 480 kb)
10681_2015_1502_MOESM2_ESM.doc (208 kb)
Supplementary material 2 (DOC 208 kb)
10681_2015_1502_MOESM3_ESM.doc (706 kb)
Supplementary material 3 (DOC 706 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • G. H. Yang
    • 1
  • Y. B. Dong
    • 1
  • Y. L. Li
    • 1
  • Q. L. Wang
    • 1
  • Q. L. Shi
    • 1
  • Q. Zhou
    • 1
  1. 1.Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, College of AgricultureHenan Agricultural UniversityZhengzhouChina

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