, Volume 206, Issue 2, pp 331–342 | Cite as

Variation and genetic stability analyses of transgenic TaLEA poplar clones from four different sites in China

  • Mengran Liu
  • Shaopeng Yin
  • Dongjing Si
  • Longting Shao
  • Ying Li
  • Mi Zheng
  • Fuwei Wang
  • Shuchun Li
  • Guifeng Liu
  • Xiyang ZhaoEmail author


Scientists have widely applied transgenic technology to plants. We transferred the TaLEA gene to Populus simonii × P. nigra (Xiaohei Poplar), obtained ten transgenic poplar clones and analyzed these clones in a greenhouse and woodland. The heights (H), basal diameters (BD), and diameter at breast high (DBH) were significantly different (P < 0.01) among the 11 test clones (the ten above and one non-transgenic clone, the control). Trees from the Xinhua site, that had low soil pH and conductivity, showed the largest H and DBH. Clone XL-1 showed the highest H, BD and DBH among the test 11 clones for 1-year-old or 3-years-old trees. The phenotypic coefficient of variation and repeatability (R) of all the traits ranged from 19.33 to 41.22 % and 0.772 to 0.965, respectively. AMMI analysis results showed that genotype (G), environment (E) and G × E interaction were highly significantly correlated (P < 0.01). Stability analysis indicated some clones that produced tall or average trees were sensitive to or resistant to adverse environmental conditions, respectively. These results suggested that the number of copies of TaLEA gene or the different integration site of each clone were not exactly same, resulting in a variety of genetic and phenotype effects. The research can provide theoretical basis for tree genetic in saline.


Populus simonii × P. nigra TaLEA Stability Genotype AMMI 



We acknowledge the Fundamental Research Funds for the Central Universities (No. 2572014CA16) and China Postdoctoral Science Foundation (2014M561315) support for the research.


  1. Balestre M, Pinho R, Soouza J, Oliverira R (2009) Genotypic stability and adaptability in tropical maize based on AMMI and GGE biplot analysis. Genet Mol Res 8(4):1311–1322CrossRefPubMedGoogle Scholar
  2. Battaglia M, Olvera CY, Garciarrubio A, Campos F, Covarrubias A (2008) The enigmatic LEA proteins and other hydrophilins. Plant Physiol 148(1):6–24PubMedCentralCrossRefPubMedGoogle Scholar
  3. Bi CX, Guo JZ, Wang HW, Shu QY (2000) The correlation and path analysis on the quantitaty characters of Chinese pine. J Northwest For Univ 15(2):7–12Google Scholar
  4. Burdon R (1977) Genetic correlation as concept for studying genotype-environment interactions in forest breeding. Silvae Genet 26:168–175Google Scholar
  5. Campos F, Zamudio F, Covarrubias A (2006) Two different late embryogenesis abundant proteins from Arabidopsis thaliana contain specific domains that inhibit Escherichia coli growth. Biochem Biophys Res Commun 342:406–413CrossRefPubMedGoogle Scholar
  6. Confalonieri M, Balestrazzi A, Bisoffi S, Carbonera D (2003) In vitro culture and genetic engineering of Populus spp.: synergy for forest tree improvement. Plant Cell Tissue Organ Cult 72:109–138CrossRefGoogle Scholar
  7. Dhillon G, Singh A, Sidhu DS, Brar HS (2012) Variation among poplar clones for growth and crown traits under field conditions at two sites of north-western India. J For Res 24:61–67CrossRefGoogle Scholar
  8. Eberhart S, Russell W (1966) Stability parameters for comparing varieties. Crop Sci 6:36–40CrossRefGoogle Scholar
  9. Fan SH, Liu GL, Zhang Q, Feng HX, Zong YC, Ren HQ (2010) A study on biomass and productivity of Populus × xiaohei plantation on study land in north China. For Res 23(1):71–76Google Scholar
  10. Filichikin S, Wu Q, Busov V, Meilan R, Garcia C, Groover A, Goldfarb B, Ma CP, Dharmawardhana P, Brunner A, Strauss S (2006) Enhancer trapping in woody plants: isolation of the ET304 gene encoding a putative AT-hook motif transcription factor and characterization of the expression patterns conferred by its promoter in transgenic Populus and Arabidopsis. Plant Sci 171:206–216CrossRefGoogle Scholar
  11. Frew M (2003) Yield stability in common bean (Phaseolus vulgaris L.) genotypes. Euphytica 130:147–153CrossRefGoogle Scholar
  12. Gal TZ, Glazer I, Koltai H (2004) An LEA group 3 family member is involved in survival of C. elegans during exposure to stress. FEBS Lett 577:21–26CrossRefPubMedGoogle Scholar
  13. Galau GA, Hughes DW, Dure L (1986) Abscisic-acid induction of cloned cotton late embryogenesis-abundant (LEA) messenger-RNAs. Plant Mol Biol 7:155–170CrossRefPubMedGoogle Scholar
  14. Gauch HG Jr, Zobel RW (1996) AMMI analysis of yield trials. In: Kang MS, Gauch HG (eds) Genotype-by-environment interaction. CRC Press, Boca RatonCrossRefGoogle Scholar
  15. Goncalves P, Bortoletto N, Cardinal A, Gouvea L, Costa R, Moraes M (2005) Age-age correlation for early selection of rubber tree genotypes in Sao Paulo State, Brazil. Genet Mol Biol 28:758–764Google Scholar
  16. Hai PH, Jansson G, Harwood C, Hannrup B, Thinh HH (2008) Genetic variation in growth, stem straightness and branch thickness in clonal trials of Acacia auriculiformis at three contrasting sites in Vietnam. For Ecol Manag 255:156–167CrossRefGoogle Scholar
  17. Hansen J, Roulund H (1997) Genetic parameters for spiral grain, stem form, pilodyn and growth in 13 years old clones of Sitka Spruce (Picea sitchensis (Bong.) Carr.). Silvae Genet 46:107–113Google Scholar
  18. Herschbach C, Zalm E, Schneider A, Jouanin L, Kok L, Rennenberg H (2000) Regulation of sulfur nutrition in wild-type and transgenic poplar over-expressingγ-glutamylcysteine synthetase in the cytosol as affected by atmospheric H2S. Plant Physiol 124:461–473PubMedCentralCrossRefPubMedGoogle Scholar
  19. Hundermark M, Hincha DK (2008) LEA (Late embryogenesis abundant) proteins and their encoding genes in Arabidopsis thaliana. BMC Genom 9:118. doi: 10.1186/1471-2164-9-118 CrossRefGoogle Scholar
  20. Jiang J, Chang YG, Dong JX, Wang ZY, Liu GF (2004) Study on two insecticidal transgenic genes in populous simonii × P. nigra. Plant Physiol Commun 40(6):669–672Google Scholar
  21. Jiang ZH, Fan SH, Feng HX, Zhang Q, Liu GL, Zong YC (2007) Biomass and distribution patterns of Populus × xiaohei plantation in sandy land of north China. Sci Silvae Sin 43(11):15–20Google Scholar
  22. Johson GR (1997) Site-to-site genetic correlations and their implications on breeding zone size and optimum munber of progeny test sites for coastal douglas-fir. Silvae Genet 46:280–285Google Scholar
  23. Kang M, Pham H (1991) Simultaneous selection for high yielding and stable crop genotypes. Agron J 83:161–165CrossRefGoogle Scholar
  24. Kempton RA (1984) The use of the biplots in interpreting variety by environment interactions. J Agric Sci 103:123–135CrossRefGoogle Scholar
  25. Kien ND Jansson, Harwood C, Almqxist C, Thinh HH (2008) Genetic variation in wood basic density and Pilodyn penetration and their relationships with growth, stem straightness and branch size for Eucalyptus urophylla S. T. Blake in Northern Vietnam. NZ J For Sci 38:160–175Google Scholar
  26. Krualee S, Sdoodee S, Eksomtramage T, Sereeprasert V (2012) Stability of fresh fruit bunch of oil palm cross (Elaeis Guineensis Jacq.) in southern Thailand. Sabrao J Breed Genet 44:1–8Google Scholar
  27. Li XX, Wang JJ, Pang ZW, Zhao XJ, Li YC (1998) Evaluation of growth and management density for Yingchun poplar. J Northeast For Univ 26(6):24–27Google Scholar
  28. Li ZX, Zhao XY, Yang CJ, Wang GY, Wang FS, Zhang LF, Zhang LC, Liu GF, Jiang J (2013) Variation and growth adaptability analysis of transgenic populous simonii × P. nigra. clones carrying TaLEA gene. J Beijing For Univ 35(2):57–62Google Scholar
  29. Lin SZ, Zhang ZY, Zhang Q, Lin YZ (2006) Progress in the study of molecular genetic improvements of poplar in China. J Integr Plant Biol 48(9):1001–1007CrossRefGoogle Scholar
  30. Maniee M, Kahrizi D, Mohammadi H (2009) Genetic variability of some morphophysiological traits in durum wheat (Triticum turgidum var. durum). J Appl Sci 9:1383–1387CrossRefGoogle Scholar
  31. Marron N, Ceulemans R (2006) Genetic variation of leaf traits related to productivity in a Populus deltoides × Populus nigra family. Can J For Res 36:390–400CrossRefGoogle Scholar
  32. Misra RC, Das S, Patnaik MC (2009) AMMI model analysis of stability and adaptability of late duration finger millet (Eleusine coracana) genotypes. World Appl Sci J 6:1650–1654Google Scholar
  33. Ortiz R, Wagoire WW, Hill J, Chandra S, Madsen S, Stolen O (2001) Heritability of and correlations among genotype-by-environment stability statistics for grain yield in bread wheat. Theor Appl Genet 103:469–474CrossRefGoogle Scholar
  34. Pliura A, Zhang SY, Mackay J, Bousquet J (2007) Genotypic variation in wood density and growth traits of poplar hybrids at four clonal trials. For Ecol Manag 238:92–106CrossRefGoogle Scholar
  35. Salvaev R, Rekoslavskaya N, Chepinoga A, Mapelli S, Pacovsky R (2006) Transgenic poplar with enhanced growth by introduction of the ugt and acb genes. New For 32:211–229CrossRefGoogle Scholar
  36. Singh S, Cornilescu CC, Tyler RC, Cornilescu G, Tonelli M, Lee M (2005) Solution structure of a late embryogenesis abundant protein (LEA14) from Arabidopsis thaliana, a cellular stress-related protein. Protein Sci 14:2601–2609PubMedCentralCrossRefPubMedGoogle Scholar
  37. Su XH, Chu YG, Li H, Hou YJ, Zhang BY, Huang QJ, Hu ZM, Huang RF, Tian YC (2011) Expression of multiple resistance genes enhances tolerance to environmental stressors in transgenic poplar (Populus × euramericana ‘Guariento’). PLoS One 6(9):e24614PubMedCentralCrossRefPubMedGoogle Scholar
  38. Sumida A, Miyaura T, Hitoshi T (2013) Relationships of tree height and diameter at breast height revisited: analyses of stem growth using 20-year data of an even-aged Chamaecyparis obtuse stand. Tree Physiol 33:106–118PubMedCentralCrossRefPubMedGoogle Scholar
  39. Taniguchi T, Ohmiya Y, Kurita M, Tsubomura M, Kondo T, Park Y, Baba K, Hayashi T (2008) Biosafety assessment of transgenic poplars overexpressing xyloglucanase (AaXEG2) prior to field trials. J Wood Sci 54:408–413CrossRefGoogle Scholar
  40. Velten J, Oliver MJ (2001) Tr288, a rehydrin with a dehydrin twist. Plant Mol Biol 45(6):713–722CrossRefPubMedGoogle Scholar
  41. Wang L, Zhou BR, Wu LL, Lv CY, Qu YJ, Zheng W, Jiang TB (2009) Cloning and expression analysis of a ring zinc-finger gene in populous simonii × P. nigra. Plant Physiol Commun 45(12):1160–1166Google Scholar
  42. Wang S, Liu MR, Huang HJ, Mu HZ, Li ZX, Liu GF (2011) Selection of cold resistant strains from TaLEA gene transferred populous simonii × P. nigra. J Northeast For Univ 39(9): 5–7,16Google Scholar
  43. Xu JR (2006) Quantitative genetics in forestry. Higher Education Press, Beijing, pp 17–18Google Scholar
  44. Yan W (2001) GGE Biplot-A Windows application for graphical analysis of multi-environment trial data and other types of two-way data. Agron J 93:1111–1118CrossRefGoogle Scholar
  45. Yang CP, Liu GF, Liang HW, Zhang H (2001) Study on the transformation of Populous simonii × P. nigra with salt resistance gene BET-A. Sci Silvae Sin 37(6):34–38Google Scholar
  46. Yu QB, Pulkkien P (2003) Genotype-environment interaction and stability in growth of aspen hybrid clones. For Ecol Manag 173:25–35CrossRefGoogle Scholar
  47. Zhao XY, Hou W, Zheng HQ, Zhang ZY (2014) Analyses of genotypic variation in white poplar clones at four sites in China. Silvae Genet 62(4–5):187–195Google Scholar
  48. Zobel RW, Madison JW, Gauch HG (1988) Statistical analysis of a yield trial. Agron J 80:388–393CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Mengran Liu
    • 1
  • Shaopeng Yin
    • 1
  • Dongjing Si
    • 1
  • Longting Shao
    • 1
  • Ying Li
    • 1
  • Mi Zheng
    • 1
  • Fuwei Wang
    • 2
  • Shuchun Li
    • 2
  • Guifeng Liu
    • 1
  • Xiyang Zhao
    • 1
    Email author
  1. 1.State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University)Northeast Forestry UniversityHarbinPeople’s Republic of China
  2. 2.Tree Seedling Management StationThe Forestry Department of Jilin ProvinceChangchunPeople’s Republic of China

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