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Euphytica

, Volume 206, Issue 3, pp 609–617 | Cite as

Genetic analysis and molecular mapping of genes controlling seed coat colour in adzuki bean (Vigna angularis)

  • Yuki Horiuchi
  • Hiroki Yamamoto
  • Reina Ogura
  • Naomi Shimoda
  • Hitoshi Sato
  • Kiyoaki KatoEmail author
Article

Abstract

Seed coat colour is an important agronomic trait in adzuki bean (Vigna angularis). Here, we identified four genes controlling seed coat colour in this species. We conducted a quantitative trait locus (QTL) study of 133 recombinant inbred lines (F6) derived from a cross between V. angularis Shumari (red) and landrace Acc2265 (olive buff), by using simple sequence repeat (SSR) markers. We identified a strong QTL, designated OLB1, which explained 54, 43, and 56 % of the total variance in the L* (lightness), a* (redness), and b* (yellowness) values, respectively. In addition, we identified a minor QTL, designated OLB2, which explained 6 % of the total variance in redness. OLB1 and OLB2 were located on linkage group (LG) 1, >80 cM apart. The F1 phenotype was olive buff, implying that the red colour trait was recessive. Next, we crossed two spontaneous mutants, ‘Shiro-shouzu’ (ivory yellow) and ‘TA230002’ (pale olive buff), to the red-seeded ‘Beni-shouzu’. Genetic analysis of the F1, F2, and F3 progenies derived from each parental cross revealed that the ivory yellow and pale olive buff traits were recessive to the red trait. Molecular mapping studies indicated that the ivory yellow trait was controlled by a single Mendelian gene, designated IVY, located on LG 8. The pale olive buff trait was also controlled by a single Mendelian gene, designated POB, located on LG 10. Our findings will facilitate the development of novel red seed cultivars and the introgression of ivory yellow genes into desirable cultivars, via marker-assisted selection.

Keywords

Adzuki bean Seed quality Seed coat colour Natural variation Molecular map 

Notes

Acknowledgments

We thank Tokachi Agricultural Cooperative Associations and the Hokkaido College of Agriculture for providing the original seeds of ‘TA230002’ and ‘Shiro-shouzu’, respectively.

Conflict of interest

The authors declare that they have no conflict of interest.

Funding

This study was funded by the Japan Beans Fund Association and the Obihiro University of Agriculture and Veterinary Medicine (10089).

Supplementary material

10681_2015_1461_MOESM1_ESM.pptx (1 mb)
Supplementary material 1 (PPTX 1042 kb). F3 seeds of a cross between adzuki bean (Vigna angularis) cultivars ‘Shumari’ (red) and Acc2265 (olive buff)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Yuki Horiuchi
    • 1
  • Hiroki Yamamoto
    • 2
  • Reina Ogura
    • 3
  • Naomi Shimoda
    • 2
  • Hitoshi Sato
    • 1
  • Kiyoaki Kato
    • 2
    Email author
  1. 1.Tokachi Agricultural Experiment Station, Agricultural Research DepartmentHokkaido Research OrganizationMemuroJapan
  2. 2.Department of Agro-Environmental ScienceObihiro University of Agriculture and Veterinary MedicineObihiroJapan
  3. 3.Central Agricultural Experiment Station, Agricultural Research DepartmentHokkaido Research OrganizationNaganumaJapan

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