, Volume 205, Issue 1, pp 269–274 | Cite as

Fine mapping and candidate identification of SST, a gene controlling seedling salt tolerance in rice (Oryza sativa L.)

  • Tao Lan
  • Shujun Zhang
  • Tingting Liu
  • Bin Wang
  • Huazhong Guan
  • Yuanchang Zhou
  • Yuanlin Duan
  • Weiren WuEmail author


Using a recessive mutant with enhanced salt tolerance at seedling stage obtained from an indica rice cultivar R401 by gamma-ray irradiation, a novel gene controlling salt tolerance in rice was previously mapped to a 406-kb region on chromosome 6. We named the gene Seedling Salt Tolerance (SST). In this study, with a large F2 population derived from a cross between mutant sst and a japonica cultivar Nipponbare (salt sensitive), SST was further fine mapped to a 17-kb interval between InDel markers ID27101 and ID27118, in which only one gene (OsSPL10) was predicted. Sequencing analysis indicated that the 232nd base of the coding sequence of OsSPL10 was deleted in the sst allele, resulting in a frameshift mutation. The result strongly suggested that OsSPL10 should be the candidate of SST. OsSPL10 is a member of the SBP-box gene family. This is the first time that the SBP-box gene family is found to be probably involved in the regulation of seedling salt tolerance in plant.


Rice Salt tolerance Gene fine mapping SPL protein 



This work was supported financially by the National Natural Science Foundation of China (Grant No. 31071399), Fujian Natural Science Foundation (Grant No. 2011J01078), and Fujian Science and Technology Project (Grant No. JK2012014).


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Tao Lan
    • 1
    • 2
  • Shujun Zhang
    • 1
  • Tingting Liu
    • 1
  • Bin Wang
    • 1
  • Huazhong Guan
    • 1
  • Yuanchang Zhou
    • 1
  • Yuanlin Duan
    • 1
  • Weiren Wu
    • 1
    • 2
    Email author
  1. 1.Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of CropsFujian Agriculture and Forestry UniversityFuzhouChina
  2. 2.Fujian Provincial Key Laboratory of Crop Breeding by DesignFujian Agriculture and Forestry UniversityFuzhouChina

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