Fine mapping and candidate identification of SST, a gene controlling seedling salt tolerance in rice (Oryza sativa L.)
- 362 Downloads
Using a recessive mutant with enhanced salt tolerance at seedling stage obtained from an indica rice cultivar R401 by gamma-ray irradiation, a novel gene controlling salt tolerance in rice was previously mapped to a 406-kb region on chromosome 6. We named the gene Seedling Salt Tolerance (SST). In this study, with a large F2 population derived from a cross between mutant sst and a japonica cultivar Nipponbare (salt sensitive), SST was further fine mapped to a 17-kb interval between InDel markers ID27101 and ID27118, in which only one gene (OsSPL10) was predicted. Sequencing analysis indicated that the 232nd base of the coding sequence of OsSPL10 was deleted in the sst allele, resulting in a frameshift mutation. The result strongly suggested that OsSPL10 should be the candidate of SST. OsSPL10 is a member of the SBP-box gene family. This is the first time that the SBP-box gene family is found to be probably involved in the regulation of seedling salt tolerance in plant.
KeywordsRice Salt tolerance Gene fine mapping SPL protein
This work was supported financially by the National Natural Science Foundation of China (Grant No. 31071399), Fujian Natural Science Foundation (Grant No. 2011J01078), and Fujian Science and Technology Project (Grant No. JK2012014).
- Gu XY, Mei MT, Yan XL, Zhen SL, Lu YG (2000) Preliminary detection of quantitative trait loci for salt tolerance in rice. Chin J Rice Sci 14:65–70Google Scholar
- Hou HM, Li J, Gao M, Singer SD, Wang H, Mao LY, Fei ZJ, Wang XP (2013) Genomic organization, phylogenetic comparison and differential expression of the SBP-box family genes in grape. PLoS ONE 8:1–15Google Scholar
- Hu S, Tao H, Qian Q, Guo L (2012) Genetics and molecular breeding for salt-tolerance in rice. Rice Genomics Genet 3:39–49Google Scholar
- Lincoln S, Daly M, Lander E (1992) Constructing genetics maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report, CambridgeGoogle Scholar
- Pandit A, Rai V, Bal S, Sinha S, Kumar V, Chauhan M, Gautam RK, Singh R, Sharma PC, Singh AK, Gaikwad K, Sharma TR, Mohapatra T, Singh NK (2010) Combining QTL mapping and transcriptome profiling of bulked RILs for identification of functional polymorphism for salt tolerance genes in rice (Oryza sativa L.). Mol Genet Genomics 284:121–136PubMedCrossRefGoogle Scholar
- Qian YL, Wang H, Chen MY, Zhang LK, Chen BS, Cui JT, Liu HY, Zhu LH, Shi YY, Gao YM, Li ZK (2009) Detection of salt-tolerant QTL using BC2F3 yield selected introgression lines of rice (Oryza sativa L.). Mol Plant Breed 7:224–232Google Scholar
- Shen B, Jiang J, Yu WD, Fan YY, Zhuang JY (2009) QTL analysis of chlorophyll fluorescence parameters in rice seedlings under salt stress. Chin J Rice Sci 23:319–322Google Scholar
- Sun Y, Zang JP, Wang Y, Zhu LH, Mohammadhosein F, Xu JL, Li ZK (2007) Mining favorable salt-tolerant QTL from rice germplasm using a backcrossing introgression line population. Acta Agron Sin 33:1611–1617Google Scholar
- Temnykh S, Declerck G, Lukashova A, Lipovich L, Cartinhour S, McCouch S (2001) Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potential. Genome Res 11:1441–1452PubMedCentralPubMedCrossRefGoogle Scholar
- Toda Y, Tanaka M, Ogawa D, Kurata K, Kurotani KI, Habu Y, Ando T, Sugimoto K, Mitsuda N, Katoh E, Abe K, Miyao A, Hirochika H, Hattori T, Takeda S (2013) RICE SALT SENSITIVE3 Forms a ternary complex with JAZ and class-C bHLH factors and regulates jasmonate-induced gene expression and root cell elongation. Plant Cell 25:1709–1725PubMedCentralPubMedCrossRefGoogle Scholar
- Wang B, Lan T, Wu WR (2007) Mapping of QTLs for Na+ content in rice seedlings under salt stress. Chin J Rice Sci 21:585–590Google Scholar