Euphytica

, Volume 204, Issue 1, pp 63–79 | Cite as

Multiple Fusarium head blight resistance loci mapped and pyramided onto elite spring wheat Fhb1 backgrounds using an IBD-based linkage approach

  • Jonathan T. Eckard
  • Karl D. Glover
  • Mohamed Mergoum
  • James A. Anderson
  • Jose L. Gonzalez-Hernandez
Article

Abstract

Pyramiding QTL for FHB resistance from multiple sources presents an opportunity to enhance the Fhb1 derived resistance of elite spring wheat germplasm. Conventionally, pyramiding QTL using a marker assisted-selection approach requires preliminary mapping studies to identify the resistance QTL from each parental line and validation studies to assess the QTL effects in multiple genetic backgrounds. This study evaluates IBD-based linkage analysis for the purpose of mapping, validation and marker-assisted pyramiding of resistance QTL simultaneously in breeding populations. Eight hundred and twenty nine segregating F1 progeny from 43 four-way crosses among multiple sources of resistance were evaluated in the greenhouse and in field-based progeny tests. IBD-based linkage analysis was conducted using a mixed model extension of Haseman-Elston sib-pair regression. QTL for FHB resistance were identified on chromosomes 2A, 2B, 3B and 7B, cumulatively explaining 18–21 % of the variance for FHB severity in different evaluations. The QTL on chromosome 3B corresponded with Fhb1, and the QTL on chromosomes 2B and 7B are corroborated by previous mapping studies. The QTL mapped to chromosome 2A potentially represents a novel source of resistance derived from elite North American spring wheat germplasm. Marker haplotypes for these QTL regions are being used for marker-assisted selection of resistance alleles in subsequent generations of these breeding populations.

Keywords

QTL mapping QTL validation Identical-by-descent Gene pyramiding Fusarium head blight 

Notes

Acknowledgments

This manuscript was based upon work supported by the USDA-ARS (Agreement ID 59-0200-3-005). This was a cooperative project with the U.S. Wheat & Barley Scab Initiative. Partial funding was provided by the South Dakota Wheat Commission and SDSU Agricultural Experiment Station. Field testing was conducted in cooperation with programs at South Dakota State University, North Dakota State University and University of Minnesota. The authors are grateful for the technical assistance provided by personnel in those programs.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Jonathan T. Eckard
    • 1
  • Karl D. Glover
    • 1
  • Mohamed Mergoum
    • 2
  • James A. Anderson
    • 3
  • Jose L. Gonzalez-Hernandez
    • 1
  1. 1.Department of Plant SciencesSouth Dakota State UniversityBrookingsUSA
  2. 2.Department of Plant SciencesNorth Dakota State UniversityFargoUSA
  3. 3.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSaint PaulUSA

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