Euphytica

, Volume 203, Issue 1, pp 177–184 | Cite as

Soybean cadmium concentration: validation of a QTL affecting seed cadmium accumulation for improved food safety

  • Johann Vollmann
  • Tomáš Lošák
  • Martin Pachner
  • Daisuke Watanabe
  • Ludmila Musilová
  • Jaroslav Hlušek
Article

Abstract

Soybean can accumulate considerable amounts of cadmium depending on soil conditions. As low cadmium concentration of soybean is of great interest in soy food production, a major quantitative trait locus described earlier for controlling cadmium accumulation in the seed was utilized to classify early maturity genotypes with respect to cadmium uptake and to verify their cadmium accumulation level. Marker-selected soybean genotypes were then tested for cadmium accumulation in a pot experiment with three cadmium levels and at three field locations in Austria. Out of 48 soybean genotypes, 19 exhibited the microsatellite marker allele associated with low cadmium accumulation. In the pot experiment, seed cadmium concentration was below 0.15 mg kg−1 in low cadmium accumulating genotypes while it reached over 0.30 mg kg−1 in genotypes carrying the high cadmium allele. In field experiments, cadmium concentration was in the range between 0.03 and 0.16 mg kg−1, and both the cadmium accumulation locus as well as the experimental locations had a significant influence on seed cadmium. Marker-assisted selection of soybean genotypes for low cadmium accumulation could thus contribute to improved food safety which is particularly important for environments with unknown cadmium status of soil.

Keywords

Soybean Seed cadmium concentration Quantitative trait locus Heavy metal accumulation Marker assisted selection 

References

  1. Adams SV, Newcomb PA, Shafer MM, Atkinson C, Aiello Bowles EJ, Newton KM, Lampe JW (2011) Sources of cadmium exposure among healthy premenopausal women. Sci Total Environ 409:1632–1637CrossRefPubMedCentralPubMedGoogle Scholar
  2. Al Sayegh Petkovšek S, Pokorny B (2013) Lead and cadmium in mushrooms from the vicinity of two large emission sources in Slovenia. Sci Total Environ 443:944–954CrossRefGoogle Scholar
  3. Arao T, Ae N, Sugiyama M, Takahashi M (2003) Genotypic differences in cadmium uptake and distribution in soybeans. Plant Soil 251:247–253CrossRefGoogle Scholar
  4. Benitez ER, Hajika M, Yamada T, Takahashi K, Oki N, Yamada N, Nakamura T, Kanamura K (2010) A major QTL controlling seed cadmium accumulation in soybean. Crop Sci 50:1728–1734CrossRefGoogle Scholar
  5. Benitez ER, Hajika M, Takahashi R (2012) Single-base substitution in P1B-ATPase gene is associated with a major QTL for seed cadmium concentration in soybean. J Hered 103:278–286CrossRefPubMedGoogle Scholar
  6. Chen KI, Erh MH, Su NW, Liu WH, Chou CC, Cheng KC (2012) Soyfoods and soybean products: from traditional use to modern applications. Appl Microbiol Biotechnol 96:9–22CrossRefPubMedGoogle Scholar
  7. European Commission (2006) Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union, L364/5 of 20.12.2006Google Scholar
  8. Greger M, Löfstedt M (2004) Comparison of uptake and distribution of cadmium in different cultivars of bread and durum wheat. Crop Sci 44:501–507CrossRefGoogle Scholar
  9. Järup L, Åkesson A (2009) Current status of cadmium as an environmental health problem. Toxicol Appl Pharm 238:201–208CrossRefGoogle Scholar
  10. Jegadeesan S, Yu K, Poysa V, Gawalko E, Morrison MJ, Shi C, Cober E (2010) Mapping and validation of simple sequence repeat markers linked to a major gene controlling seed cadmium accumulation in soybean [Glycine max (L.) Merr]. Theor Appl Genet 121:283–294CrossRefPubMedGoogle Scholar
  11. Li X, Zhang L, Li Y, Ma L, Bu N, Ma C (2012) Changes in photosynthesis, antioxidant enzymes and lipid peroxidation in soybean seedlings exposed to UV-B radiation and/or Cd. Plant Soil 352:377–387CrossRefGoogle Scholar
  12. Mansur LM, Lark KG, Kross H, Oliveira A (1993) Interval mapping of quantitative trait loci for reproductive, morphological, and seed traits of soybean (Glycine max L.). Theor Appl Genet 86:907–913PubMedGoogle Scholar
  13. McLaughlin MJ, Maier NA, Rayment GE, Sparrow LA, Berg G, McKay A, Milham P, Merry RH, Smart MK (1997) Cadmium in Australian potato tubers and soils. J Environ Qual 26:1644–1649CrossRefGoogle Scholar
  14. McLaughlin MJ, Parker DR, Clarke JM (1999) Metals and micronutrients—food safety issues. Field Crops Res 60:143–163CrossRefGoogle Scholar
  15. Meharg AA, Norton G, Deacon C, Williams P, Adomako EE, Price A, Zhu Y, Li G, Zhao FJ, McGrath S, Villada A, Sommella A, Mangala P, De Silva CS, Brammer H, Dasgupta T, Islam MR (2013) Variation in rice cadmium related to human exposure. Environ Sci Technol 47:5613–5618CrossRefPubMedGoogle Scholar
  16. Moon KH, Kim JY, Chang MI, Kim US, Kim SJ, Baek SH (2013) Heavy metal and amino acid contents of soybean by application of sewage and industrial sludge. J Korean Soc Food Sci Nutr 42:268–277CrossRefGoogle Scholar
  17. Öborn I, Jansson G, Johnsson L (1995) A field study on the influence of soil pH on trace element levels in spring wheat (Triticum aestivum), potatoes (Solanum tuberosum) and carrots (Daucus carota). Water Air Soil Pollut 85:835–840CrossRefGoogle Scholar
  18. Salazar MJ, Rodriguez JH, Nieto GL, Pignata ML (2012) Effects of heavy metal concentrations (Cd, Zn and Pb) in agricultural soils near different emission sources on quality, accumulation and food safety in soybean [Glycine max (L.) Merrill]. J Hazard Mater 233–234:244–253CrossRefPubMedGoogle Scholar
  19. SAS (1988) SAS STAT user’s guide, release 6.03. SAS Institute, CaryGoogle Scholar
  20. Sato T, Zahlner V, Berghofer E, Lošák T, Vollmann J (2012) Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples. Plant Breed 131:531–534CrossRefGoogle Scholar
  21. Shute T, Macfie SM (2006) Cadmium and zinc accumulation in soybean: a threat to food safety. Sci Total Environ 371:63–73CrossRefPubMedGoogle Scholar
  22. Spiegel H, Sager M, Oberforster M, Mechtler K, Stüger HP, Baumgarten A (2009) Nutritionally relevant elements in staple foods: influence of arable site versus choice of variety. Environ Geochem Health 31:549–560CrossRefPubMedGoogle Scholar
  23. Sugiyama M, Ae N, Arao T (2007) Role of roots in differences in seed cadmium concentration among soybean cultivars—proof by grafting experiment. Plant Soil 295:1–11CrossRefGoogle Scholar
  24. Vollmann J, Walter H, Sato T, Schweiger P (2011) Digital image analysis and chlorophyll metering for phenotyping the effects of nodulation in soybean. Comput Electron Agric 75:190–195CrossRefGoogle Scholar
  25. Wang X, Jiang GL, Green M, Scott RA, Hyten DL, Cregan PB (2012a) Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean. Mol Breed 30:1163–1179CrossRefGoogle Scholar
  26. Wang Y, Yu KF, Poysa V, Shi C, Zhou YH (2012b) A single point mutation in GmHMA3 affects cadmium (Cd) translocation and accumulation in soybean seeds. Molec Plant 5:1154–1156CrossRefGoogle Scholar
  27. Xiao CW (2008) Health effects of soy protein and isoflavones in humans. J Nutr 138:1244 S–1249 SGoogle Scholar
  28. Zhou Y, Xue M, Yang Z, Gong Y, Yuan J, Zhou C, Huang B (2013) High cadmium pollution risk on vegetable amaranth and a selection for pollution-safe cultivars to lower the risk. Front Environ Sci Eng 7:219–230CrossRefGoogle Scholar
  29. Zhuang P, Li ZA, Zou B, Xia HP, Wang G (2013) Heavy metal contamination in soil and soybean near the Dabaoshan mine, South China. Pedosphere 23:298–304CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Johann Vollmann
    • 1
  • Tomáš Lošák
    • 2
  • Martin Pachner
    • 1
  • Daisuke Watanabe
    • 1
  • Ludmila Musilová
    • 2
  • Jaroslav Hlušek
    • 2
  1. 1.Division of Plant Breeding, Department of Crop SciencesUniversity of Natural Resources and Life Sciences Vienna (BOKU)Tulln an der DonauAustria
  2. 2.Department of Environmentalistics and Natural Resources, Faculty of Regional Development and International StudiesMendel University in BrnoBrnoCzech Republic

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