Molecular diagnosis to identify new sources of resistance to sclerotinia blight in groundnut (Arachis hypogaea L.)
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Sclerotinia blight, caused by soil-borne fungus Sclerotinia minor Jagger, is one of the destructive diseases in groundnut. Pathogen affected plants usually displays lesions, wilt and collapse which cause high yield losses. Traditional field screening is time and resources consuming. Molecular markers associated with resistance genes offer an alternative selection technique which is relatively easy, more definite and not influenced by environmental fluctuations. In the present investigation, a marker-assisted diagnosis was done to screen 256 diverse germplasm for the presence or absence of SSR markers reported resistance or susceptibility to sclerotinia blight. One hundred and forty two genotypes from different botanical varieties were recognized as new potential sources of resistance to sclerotinia blight for field evaluation. The banding pattern related to the disease resistance is observed at high frequency in the variety vulgaris (39.4 %) and less distributed in the varieties fastigiata (38.0 %) and hypogaea (19.7 %) among the resistant genotypes in the collection. These genotypes had same banding pattern as reported for resistance germplasm. This work reports the successful application of marker-assisted diagnosis as a tool to identify resistance to sclerotinia blight in diverse collections.
KeywordsSclerotinia minor Jagger Disease resistance Pathogen Molecular markers Marker-assisted selection
This study was supported by the Ministry of Science, Industry and Technology of Turkey with the grant number of SANTEZ-01527-STZ-2012-2. We are grateful to International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Gene bank, Hyderabad, India for supplying genetic material and to the Scientific Research Projects Coordination Unit of Akdeniz University for continuous supports.
Conflict of interest
The authors declare that there are no conflicts of interest.
- Arus P, Moreno-Gonzalez J (1993) Marker-assisted selection. In: Hayward MD, Bosemark NO, Romagosa I (eds) Plant breeding: principles and prospects. Chapman and Hall, Cambridge, pp 315–331Google Scholar
- Doyle JJ, Doyle JL (1990) A rapid total DNA preparation procedure for fresh plant tissue. Focus 12:13–15Google Scholar
- FAO (2012) FAOSTAT. FAO, Rome. http://faostat.fao.org/site/567/default.aspx. Accessed 18 June 2014
- Gil SV, Harob R, Oddinoc C, Kearneyc M, Zuzac M, Marinellic A, Marcha GJ (2008) Crop management practices in the control of peanut diseases caused by soilborne fungi. Crop Prot 27:1–9Google Scholar
- Gregory W, Krapovickas A, Gregory M (1980) Structure, variation, evolution, and classification in Arachis. In: Summerfield R, Bunting A (eds) Advances in legume science. Royal Botanic Gardens, Kew, London, pp 469–481Google Scholar
- Krapovickas SA, Rigoni VA (1994) Taxonomia del genero Arachis (Leguminosae). Bonplandia 8:1–186Google Scholar
- Laemmlen F (2001) Sclerotinia diseases. Agriculture and Natural Resources, University of California Publication, 8042:1–5Google Scholar
- Liao B, Holbrook C (2007) Groundnut. In: Singh RJ (ed) Genetics resources, chromosome engineering and crop improvement, Oilseed Crops, vol 4. CRC Press, Boca Raton, pp 231–289Google Scholar
- Melouk HA, Backman PA (1995) Management of soil borne fungal pathogens. In: Melouk HA, Shokes FM (eds) Peanut health management. APS, Minnesota, pp 75–82Google Scholar
- Murty UR, Jahnavi MR (1983) Breeding potential of interspecific tetraploids in Arachis L. In: Feakin SD (ed) Proceedings of an international workshop on cytogenetics of Arachis. ICRISAT, Patancheru, pp 125–130Google Scholar
- Nagy ED, Chu Y, Guo Y, Khanal S, Tang S, Li Y, Dong W, Timper P, Taylor C, Ozias-Akins P, Holbrook CC, Beilinson V, Nielsen NC, Stalker HT, Knapp SJ (2010) Recombination is suppressed in an alien introgression on chromosome 5A of peanut harboring Rma, a dominant root knot nematode resistance gene. Mol Breed 26:357–370CrossRefGoogle Scholar
- Nigam SN, Aruna R (2008) Improving breeding efficiency for early maturity in peanut. Plant Breed Rev 30:295–316Google Scholar
- Porter DM, Melouk HM (1997) Sclerotinia blight. In: Kokalis-Burelle N, Porter DM, Rodriguez-Kabana R, Smith DH, Subrahmanyam P (eds) Compendium of peanut diseases, 2nd edn. American Phytopathological Society Press, St. Paul, pp 34–35Google Scholar