Whitebacked planthopper Sogatella furcifera (Horváth) (Homoptera: Delphacidae) resistance in rice variety Sinna Sivappu
- 426 Downloads
Whitebacked planthopper (WBPH) along with brown planthopper (BPH) has emerged as a major pest of rice in several Asian countries. Development and cultivation of varieties resistant to both planthoppers is an ecologically acceptable strategy to manage these pests. Sinna Sivappu, a Sri Lankan landrace, was reported to be resistant to both planthoppers. While inheritance of BPH resistance has been reported, the genetics of WBPH resistance in this variety is not known. Using a mapping population of 255 F2:3 families from Taichung Native (TN)1/Sinna Sivappu cross and 128 polymorphic simple sequence repeat (SSR) markers, genes or quantitative trait loci (QTLs) for WBPH resistance quantified in ten phenotypic tests were identified, adopting classical Mendelian segregation, correlation and QTL analyses. The inheritance pattern suggested that a single recessive gene controlled regulation of seedling damage score. Antixenosis or nymphal preference was influenced by two complementary recessive genes, whereas tolerance in terms of days to wilt was under the influence of a single dominant gene. Several of these phenotypic tests recorded high degree of positive or negative correlation between them, suggesting dependence or redundancy of the tests. QTL analysis revealed 13 loci associated with nine traits. Five major-effect QTLs were detected for damage score (chromosome 6), nymphal survival (chromosome 12), and days to wilt (three QTLs on chromosome 4). We suggest involvement of four WBPH resistance genes in Sinna Sivappu, designated as wbph9(t), wbph10(t), wbph11(t), and Wbph12(t). One of the recessive genes could be allelic to any of the recessive genes reported in cluster C on chromosome 6 which might confer resistance to both BPH and WBPH.
KeywordsWhitebacked planthopper SSR markers Novel gene(s) QTLs Oryza sativa L.
We thank the Project Director, Directorate of Rice Research, Hyderabad for the facilities and encouragement. This work was partly supported by a grant from the Department of Biotechnology (DBT F.No.BT/AB/FG-2 (PH-II) Sep-2009), Government of India.
- Bentur JS, Viraktamath BC (2008) Rice planthoppers strike back. A report on second international rice conference on rice planthoppers held at IRRI, Philippines during 23–25 June 2008. Curr Sci 95(4):441Google Scholar
- Directorate of Rice Research (DRR) (1999) Annual Report 1998. Directorate of Rice Research, HyderabadGoogle Scholar
- Heinrichs EA, Medrano FG, Rapusas HR (1985) Genetic evaluation for insect resistance in rice. International Rice Research Institute, Los Baños, pp 1–356Google Scholar
- IRRI (International Rice Research Institute) (1996) Standard evaluation system for rice. International Rice Research Institute, ManilaGoogle Scholar
- Kalode MB, Viswanathan PRK, Seshu DV (1975) Standard test to characterize host plant resistance to brown planthopper in rice. Indian J Plant Prot 3:204–206Google Scholar
- Kalode MB, Krishna TS, Pophalay DJ, Laxminarayana A (1977) A note on new multiple resistant donors to major insect pests of rice (Oryza sativa L.). Indian J Agric Sci 47:626–627Google Scholar
- Sidhu N, Basal UK, Shukla KK, Saini RG (2005) Genetics of resistance to whitebacked planthopper in five rice stocks. SABRAO J Breed Genet 37:43–49Google Scholar
- Sogawa K, Liu G, Qiang Q (2009) Prevalence of whitebacked planthoppers in Chinese hybrid rice and whitebacked planthopper resistance in Chinese japonica rice. In: Heong KL, Hardy B (eds) Planthoppers: New Threats to the Sustainability of Intensive Rice Production Systems in Asia. International Rice Research Institute, Los Baños, pp 257–280Google Scholar
- Van Ooijen JW (2006) JoinMap 4.0 Software for the calculation of genetic linkage maps in experimental populations. Kyzama BV, Wageningen, The NetherlandsGoogle Scholar
- Wang S, Basten CJ, Zeng ZB (2007) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC. User manual pp 1–87. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm. Accessed 10 Dec 2013
- Zheng K, Huang N, Bennett J, Khush GS (1995) PCR-based marker assisted selection in rice breeding. IRRI Discussion Paper Series No. 12. International Rice Research Institute, ManilaGoogle Scholar