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Euphytica

, Volume 197, Issue 2, pp 201–210 | Cite as

Molecular cytogenetic characteristics of a translocation line between common wheat and Thinopyrum intermedium with resistance to powdery mildew

  • Xueqin Tang
  • Dong Shi
  • Jie Xu
  • Yinglu Li
  • Wenjing Li
  • Zhenglong Ren
  • Tihua FuEmail author
Article

Abstract

Powdery mildew caused by Blumeria graminis (DC) Speer f. sp. tritici Em. Marchal is a serious disease of wheat (Triticum aestivum L.) in Southwestern China. A line of common wheat designated 08-723 isolated from the progeny of a hybrid between common wheat and Thinopyrum intermedium (Host) Barkworth & Dewey, was highly resistant to the existing powdery mildew races in the region. This line had a similar phenotype to its wheat parent, and it showed normal bivalent pairing at metaphase I of meiosis. It was analyzed by genomic in situ hybridization, fluorescence in situ hybridization and sequential C-banding-GISH to determine the amount, location and origin of the alien chromatin present. The results revealed that line 08-723 is homozygous for a two-point translocation replacing chromosome 6A of wheat. The translocation chromosome appears to have a normal 6AL arm; its short arm has a short terminal segment of ca. 10 % in length originating from an unidentified B-genome chromosome of wheat and a long proximal segment of ca. 90 % of the arms’ length originating from one of the St-genome chromosomes of Th. intermedium. Genetic analysis of powdery mildew resistance in F1, F2 and F2:3 populations from a cross of 08-723 with a susceptible wheat line indicated that the resistance was controlled by a single dominant gene and in a sample of F2 plants it always associated with the translocated chromosome. The gene responsible for resistance on the translocated chromosome may provide an alternate source of resistance in wheat breeding programs.

Keywords

Double translocation ISH Powdery mildew resistance Sequential C-banding-GISH Thinopyrum intermedium 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China. We particularly thank Prof. Shulan Fu for providing the pSc119.2 and pAs1 repetitive sequences, and the genomic DNA of Th. elongatum, T. urartu, A. speltoides and A. Tauschii. We also thank Prof. Yingze Niu for revising the manuscript.

References

  1. Ayala-Navarrete LI, Mechanicos AA, Gibson JM, Singh D, Bariana HS, Fletcher J, Shorter S, Larkin PJ (2013) The Pontin series of recombinant alien translocations in bread wheat: single translocations integrating combinations of Bdv2, Lr19 and Sr25 disease-resistance genes from Thinopyrum intermedium and Th. ponticum. Theor Appl Genet 126(10):2467–2475PubMedCrossRefGoogle Scholar
  2. Chen Q (2005) Detection of alien chromatin introgression from Thinopyrum into wheat using S genomic DNA as a probe-A landmark approach for Thinopyrum genome research. Cytogenet Gen Res 109:350–359CrossRefGoogle Scholar
  3. Chen Q, Friebe B, Conner RL, Laroche A, Thomas JB, Gill BS (1998) Molecular cytogenetic characterization of Thinopyrum intermedium-derived wheat germplasm specifying resistance to wheat streak mosaic virus. Theor Appl Genet 96:1–7CrossRefGoogle Scholar
  4. Chen XM, Luo YH, Xia XC, Xia LQ, Chen X, Ren ZL, He ZH, Jia JZ (2005) Chromosomal location of powdery mildew resistance gene Pm16 in wheat using SSR marker analysis. Plant Breed 124:225–228CrossRefGoogle Scholar
  5. Everts KL, Leath S (1992) Effect of early season powdery mildew on development, survival, and yield contribution of tillers of winter wheat. Phytopathology 82:1273–1278CrossRefGoogle Scholar
  6. Fedak G, Han F (2005) Characterization of derivatives from wheat-Thinopyrum wide crosses. Cytogenet Gen Res 109:350–359CrossRefGoogle Scholar
  7. Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87CrossRefGoogle Scholar
  8. Fu SL, Lv ZL, Qi B, Guo X, Li J, Liu B, Han FP (2012) Molecular cytogenetic characterization of wheat- Thinopyrum elongatum addition, substitution and translocation lines with a novel source of resistance to wheat fusarium head blight. J Genet Genomics 39:103–110PubMedCrossRefGoogle Scholar
  9. Gill BS, Friebe B, Endo TR (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome 34:830–839CrossRefGoogle Scholar
  10. Hao YF, Liu AF, Wang YH, Feng DS, Gao JR, Li XF, Liu SB, Wang HG (2008) Pm23: a new allele of Pm4 located on chromosome 2AL in wheat. Theor Appl Genet 117(8):1205–1212PubMedCrossRefGoogle Scholar
  11. Hao M, Luo JT, Yang M, Zhang LQ, Yan ZH, Yuan ZW, Zheng YL, Zhang HG, Liu DC (2011) Comparison of homoeologous chromosome pairing between hybrids of wheat genotypes Chinese Spring ph1band Kaixian luohanmai with rye. Genome 54:959–964PubMedCrossRefGoogle Scholar
  12. He R, Chang Z, Yang Z, Yuan Z, Zhan H, Zhang X, Liu J (2009) Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat. Theor Appl Genet 118:1173–1180PubMedCrossRefGoogle Scholar
  13. Huang XQ, Röder MS (2004) Molecular mapping of powdery mildew resistance genes in wheat: a review. Euphytica 137:203–223CrossRefGoogle Scholar
  14. Jiang J, Gill BS (1993) A “zebra” chromosome arising from multiple translocations involving non-homologous chromosomes. Chromosoma 102(9):612–617PubMedCrossRefGoogle Scholar
  15. Kato A, Lamb JC, Birchler JA (2004) Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proc Natl Acad Sci USA 101:13554–13559PubMedCentralPubMedCrossRefGoogle Scholar
  16. Komuro S, Endo R, Shikata K, Kato A (2013) Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure. Genome 56(3):131–137PubMedCrossRefGoogle Scholar
  17. Li HJ, Wang XM (2009) Thinopyrum ponticum and Th. intermedium: the promising source of resistance to fungal and viral diseases of wheat. J Genet Genomics 36:557–565PubMedCrossRefGoogle Scholar
  18. Liu SB, Wang HG (2005) Characterization of a wheat-Thinopyron intermedium substitution line with resistance to powdery mildew. Euphytica 143:229–233CrossRefGoogle Scholar
  19. Liu SB, Wang HG, Zhang XY, Li XF, Li DY, Duan XY, Zhou YL (2005) Molecular cytogenetic identification of a wheat- Thinopyron intermedium (Host) Barkworth & DR Dewey partial amphiploid resistant to powdery mildew. J Integr Plant Biol 47(6):726–733CrossRefGoogle Scholar
  20. Liu J, Chang ZJ, Zhang XJ, Yang ZJ, Li X, Jia JQ, Zhan HX, Guo HJ, Wang JM (2013) Putative Thinopyrum intermedium -derived stripe rust resistance gene Yr50 maps on wheat chromosome arm 4BL. Theor Appl Genet 126:265–274PubMedCrossRefGoogle Scholar
  21. Lukaszewski AJ (1993) Reconstruction in wheat of complete chromosomes 1B and 1R from the 1RS.1BL translocation of ‘Kavkaz’ origin. Genome 36:821–824PubMedCrossRefGoogle Scholar
  22. Lukaszewski AJ (1995a) Physical distribution of translocation breakpoints in homoeologous recombinants induced by the absence of the Ph1 gene in wheat and triticale. Theor Appl Genet 90:714–719PubMedCrossRefGoogle Scholar
  23. Lukaszewski AJ (1995b) Chromatid and chromosome type breakage-fusion-bridge cycles in wheat (Triticum aestiuum L.). Genetics 140:1069–1085PubMedCentralPubMedGoogle Scholar
  24. Luo MC, Dubcovsky J, Goyal S, Dvorak J (1996) Engineering of interstitial foreign chromosome segments containing the K+/Na+ selectivity gene Kna1 by sequential homoeologous recombination in durum wheat. Theor Appl Genet 93:1180–1184PubMedCrossRefGoogle Scholar
  25. Luo P, Luo H, Chang Z, Zhang H, Zhang M, Ren Z (2009) Characterization and chromosomal location of Pm40 in common wheat: a new gene for resistance to powdery mildew derived from Elytrigia intermedium. Theor Appl Genet 118:1059–1064PubMedCrossRefGoogle Scholar
  26. Mahelka V, Kopeck D, Paštová L (2011) On the genome constitution and evolution of intermediate wheatgrass (Thinopyrum intermedium: Poaceae, Triticeae). BMC Evol Biol 11:127–143PubMedCentralPubMedCrossRefGoogle Scholar
  27. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalogue of gene symbols for wheat. In: Proceedings of the 11th international wheat genetic symposium. University of Sydney Press, AustraliaGoogle Scholar
  28. McIntyre CL, Pereira S, Moran LB, Appels R (1990) New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome 33:635–640PubMedCrossRefGoogle Scholar
  29. Nagaki K, Tsujimoto H, Isono K, Sasakuma T (1995) Molecular characterization of a tandem repeat, Afa family, and its distribution among Triticeae. Genome 38:479–486PubMedCrossRefGoogle Scholar
  30. Niewoehner AS, Leath S (1998) Virulence of Blumeria graminis f. sp. tritici on winter wheat in the eastern United States. Plant Dis 82:64–68CrossRefGoogle Scholar
  31. Qi LL, Pumphrey MO, Friebe B, Zhang P, Qian C, Bowden RL, Rouse MN, Jin Y, Gill BS (2011) A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theor Appl Genet 123:159–167PubMedCrossRefGoogle Scholar
  32. Rayburn AL, Gill BS (1986) Isolation of a D genome specific repeated DNA sequence from Aegilops squarrosa. Plant Mol Biol Rep 4:102–109CrossRefGoogle Scholar
  33. Riley R, Chapman V, Johnson R (1968a) The incorporation of alien disease resistance in wheat by genetic interference with the regulation of meiotic chromosome synapsis. Genet Res Camb 12:198–219CrossRefGoogle Scholar
  34. Riley R, Chapman V, Johnson R (1968b) Introduction of yellow rust resistance of Aegilops comosa into wheat by genetically induced homoeologous recombination. Nature 217:383–384CrossRefGoogle Scholar
  35. Robertson WMRB (1916) Chromosome studies. I. Taxonomic relationships shown in the chromosomes of Tettegidae and Acrididiae: V-shaped chromosomes and their significance in Acrididae, Locustidae and Grillidae: chromosomes and variations. J Morphol 27:179–331CrossRefGoogle Scholar
  36. Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann Bot 64(3):315–324Google Scholar
  37. Sears ER (1952) Misdivision of univalents in common wheat. Chromosoma 4:535–550PubMedCrossRefGoogle Scholar
  38. Sears ER (1954) Aneuploids of common wheat. Research Bulletin 572. University of Missouri Agricultural Experimental Station, Columbia, pp 1–59Google Scholar
  39. Sears ER (1956) The transfer of leaf rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp Biol 9:1–22Google Scholar
  40. Sepsi A, Molnár I, Szalay D, Molnár-Láng M (2008) Characterization of a leaf rust-resistant wheat-Thinopyrum ponticum partial amphiploid BE-1, using sequential multicolor GISH and FISH. Theor Appl Genet 116(6):825–834PubMedCrossRefGoogle Scholar
  41. Song W, Xie H, Liu Q, Xie CJ, Ni ZF, Yang S, Sun QX, Liu XY (2007) Molecular identification of Pm12 -carrying introgression lines in wheat using genomic and EST-SSR markers. Euphytica 158:95–102CrossRefGoogle Scholar
  42. Trask BJ (1991) Fluorescence in situ hybridization: applications in cytogenetics and gene mapping. Trends Genet 7(5):149–154PubMedCrossRefGoogle Scholar
  43. Wang RRC, Zhang XY (1996) Characterization of the translocated chromosome using fluorescence in situ hybridization and random amplified polymorphic DNA on two Triticum aestivum-Thinopyrum intermedium translocation lines resistant to wheat streak mosaic or barley yellow dwarf virus. Chromosome Res 4(8):583–587PubMedCrossRefGoogle Scholar
  44. Weimarck A (1974) Elimination of wheat and rye chromosomes in a strain of octoploid triticale as revealed by Giemsa banding technique. Hereditas 77:281–286PubMedCrossRefGoogle Scholar
  45. Xiao MG, Song FJ, Jiao JF, Wang XM, Xu HX, Li HJ (2013) Identification of the gene Pm47 on chromosome 7BS conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi. Theor Appl Genet 126:1397–1403PubMedCrossRefGoogle Scholar
  46. Xu LS, Wang MN, Cheng P, Kang ZS, Hulbert SH, Chen XM (2013) Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat. Theor Appl Genet 126(2):523–533PubMedCrossRefGoogle Scholar
  47. Xue F, Wang CY, Li C, Duan XY, Zhou YL, Zhao NJ, Wang YJ, Ji WQ (2012) Molecular mapping of a powdery mildew resistance gene in common wheat landrace Baihulu and its allelism with Pm24. Theor Appl Genet 125(7):1425–1432PubMedCrossRefGoogle Scholar
  48. Zeng J, Cao W, Fedak G, Sun S, McCallum B, Fetch T, Xue A, Zhou Y (2013) Molecular cytological characterization of two novel durum–Thinopyrum intermedium partial amphiploids with resistance to leaf rust, stem rust and Fusarium head blight. Hereditas 151(1):10–16CrossRefGoogle Scholar
  49. Zhao YL, Fu TH, Ren ZL (2001) Influence of bands on critical treatment factors in the Giemsa-C banding procedure for Triticeae chromosomes. J Sichuan Agri Univ 10(3):206–210Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Xueqin Tang
    • 1
  • Dong Shi
    • 1
  • Jie Xu
    • 1
  • Yinglu Li
    • 1
  • Wenjing Li
    • 1
  • Zhenglong Ren
    • 1
  • Tihua Fu
    • 1
    Email author
  1. 1.Agronomy CollegeSichuan Agricultural UniversityWenjiangPeople’s Republic of China

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