Characterization of Chinese wheat germplasm for resistance to Fusarium head blight at CIMMYT, Mexico
- 716 Downloads
Fusarium head blight (FHB) poses a challenge for wheat breeders worldwide; there are limited sources of resistance and the genetic basis for resistance is not well understood. In the mid-1980s, a shuttle breeding and germplasm exchange program launched between CIMMYT-Mexico and China, enabled the incorporation of FHB resistance from Chinese bread wheat germplasm into CIMMYT wheat. Most of the Chinese wheat materials conserved in the CIMMYT germplasm bank had not been fully characterized for FHB reaction under Mexican environments, until 2009, when 491 Chinese bread wheat lines were evaluated in a FHB screening nursery in Mexico, and 304 (61.9 %) showed FHB indices below 10 %. Subsequent testing occurred in 2010 for plant height (PH), days to heading (DH), and leaf rust response. In 2012, 140 elite lines with good agronomic types were further evaluated for field FHB reaction and deoxynivalenol (DON) accumulation. Most of the tested lines showed good resistance: 116 (82.9 %) entries displayed FHB indices lower than 10 %, while 89 (63.6 %) had DON contents lower than 1.0 ppm. Significant negative correlations were observed between FHB traits (FHB index, DON content, and Fusarium damaged kernels) and PH, DH, and anther extrusion. A subset of 102 elite entries was selected for haplotyping using markers linked to 10 well known FHB quantitative trait loci (QTL). 57 % of the lines possessed the same 2DL QTL marker alleles as Wuhan 1 or CJ 9306, and 26.5 % had the same 3BS QTL allele as Sumai 3. The remaining known QTL were of low frequency. These materials, especially those with none of the above tested resistance QTL (26.5 %), could be used in breeding programs as new resistance sources possessing novel genes for FHB resistance and DON tolerance.
KeywordsBread wheat Germplasm Scab Resistance breeding DON content Anther extrusion
The first author is grateful for the financial support from a CGIAR scholarship, provided by the Research Council of Norway, through NFR project 208340/H30—Breeding for Fusarium resistance in wheat. The authors acknowledge the pedigree information received from Drs. Hongxiang Ma, Jiangsu Academy of Agricultural Sciences, Nanjing; Ennian Yang, Sichuan Academy of Agricultural Sciences, Chengdu; and Siheng Liu, Fujian Agricultural University, Fuzhou; and editing assistance from Ms Emma Quilligan (CIMMYT).
- All-China Cooperation of Research on Wheat Scab (1984) Studies on scab resistance identification in wheat germplasm resources. Crop Genetic Resources 4:2–7 (in Chinese)Google Scholar
- CIMMYT (2005) Laboratory protocols: CIMMYT applied molecular genetics laboratory, 3rd edn. CIMMYT, MexicoGoogle Scholar
- Gilchrist L, Rajaram S, Mujeeb-Kazi A, van Ginkel M, Vivar H, Pfeiffer W (1996) Fusarium scab screening program at CIMMYT. In: Dubin HJ, Gilchrist L, Reeves J, McNab A (eds) Fusarium head scab: global status and future prospects. CIMMYT, Mexico D.F., pp 7–12Google Scholar
- He ZH, van Ginkel M, Gilchrist L, Rajaram S (2000) Progress of China/CIMMYT shuttle breeding and germplasm exchange aimed at combining high yield potential with scab resistance. Proc Natl Fusarium Head Blight Forum, Cincinnati, Ohio, USA, pp 264–268Google Scholar
- He ZH, Rajaram S, Xin ZY, Huang GZ (eds) (2001) A history of wheat breeding in China. CIMMYT, MexicoGoogle Scholar
- Liu ZZ, Wang ZY, Huang DC, Zhao WJ, Huang XM (1997) Improvement of CIMMYT wheat germplasm for scab resistance and its utilization in Shanghai. In: He Z, Rajaram S (eds) China/CIMMYT collaboration on wheat breeding and germplasm exchange: Results of 10 years of shuttle breeding (1984–94). Wheat Special Report No 46. CIMMYT, Mexico, pp 28–32Google Scholar
- Lu W, Cheng S, Liu Y (2001) Wheat scab research in China. Science Publishing Company, Beijing (in Chinese)Google Scholar
- Ortiz R, Braun H-J, Crossa J, Crouch JH, Davenport G, Dixon J, Dreisigacker S, Duveiller E, He Z, Huerta J, Joshi AK, Kishii M, Kosina P, Manes Y, Mezzalama M, Morgounov A, Murakami J, Nicol J, Ortiz Ferrara G, Ortiz-Monasterio JI, Payne TS, Peña RJ, Reynolds MP, Sayre KD, Sharma RC, Singh RP, Wang J, Warburton M, Wu H, Iwanaga M (2008) Wheat genetic resources enhancement by the International Maize and Wheat Improvement Center (CIMMYT). Genet Resour Crop Evol 55:1095–1140CrossRefGoogle Scholar
- Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838Google Scholar
- Zhou C (2003) Breeding for Fusarium head blight in wheat. In: Zhuang Q (ed) Chinese wheat improvement and pedigree analysis. China Agriculture Press, Beijing, pp 444–469 (in Chinese) Google Scholar