Euphytica

, Volume 195, Issue 3, pp 383–395 | Cite as

Characterization of Chinese wheat germplasm for resistance to Fusarium head blight at CIMMYT, Mexico

  • Xinyao He
  • Pawan K. Singh
  • Norbert Schlang
  • Etienne Duveiller
  • Susanne Dreisigacker
  • Thomas Payne
  • Zhonghu He
Article

Abstract

Fusarium head blight (FHB) poses a challenge for wheat breeders worldwide; there are limited sources of resistance and the genetic basis for resistance is not well understood. In the mid-1980s, a shuttle breeding and germplasm exchange program launched between CIMMYT-Mexico and China, enabled the incorporation of FHB resistance from Chinese bread wheat germplasm into CIMMYT wheat. Most of the Chinese wheat materials conserved in the CIMMYT germplasm bank had not been fully characterized for FHB reaction under Mexican environments, until 2009, when 491 Chinese bread wheat lines were evaluated in a FHB screening nursery in Mexico, and 304 (61.9 %) showed FHB indices below 10 %. Subsequent testing occurred in 2010 for plant height (PH), days to heading (DH), and leaf rust response. In 2012, 140 elite lines with good agronomic types were further evaluated for field FHB reaction and deoxynivalenol (DON) accumulation. Most of the tested lines showed good resistance: 116 (82.9 %) entries displayed FHB indices lower than 10 %, while 89 (63.6 %) had DON contents lower than 1.0 ppm. Significant negative correlations were observed between FHB traits (FHB index, DON content, and Fusarium damaged kernels) and PH, DH, and anther extrusion. A subset of 102 elite entries was selected for haplotyping using markers linked to 10 well known FHB quantitative trait loci (QTL). 57 % of the lines possessed the same 2DL QTL marker alleles as Wuhan 1 or CJ 9306, and 26.5 % had the same 3BS QTL allele as Sumai 3. The remaining known QTL were of low frequency. These materials, especially those with none of the above tested resistance QTL (26.5 %), could be used in breeding programs as new resistance sources possessing novel genes for FHB resistance and DON tolerance.

Keywords

Bread wheat Germplasm Scab Resistance breeding DON content Anther extrusion 

Notes

Acknowledgments

The first author is grateful for the financial support from a CGIAR scholarship, provided by the Research Council of Norway, through NFR project 208340/H30—Breeding for Fusarium resistance in wheat. The authors acknowledge the pedigree information received from Drs. Hongxiang Ma, Jiangsu Academy of Agricultural Sciences, Nanjing; Ennian Yang, Sichuan Academy of Agricultural Sciences, Chengdu; and Siheng Liu, Fujian Agricultural University, Fuzhou; and editing assistance from Ms Emma Quilligan (CIMMYT).

Supplementary material

10681_2013_1002_MOESM1_ESM.xlsx (36 kb)
Supplementary material 1 (XLSX 35 kb)
10681_2013_1002_MOESM2_ESM.docx (17 kb)
Supplementary material 2 (DOCX 16 kb)
10681_2013_1002_MOESM3_ESM.xlsx (31 kb)
Supplementary material 3 (XLSX 30 kb)

References

  1. All-China Cooperation of Research on Wheat Scab (1984) Studies on scab resistance identification in wheat germplasm resources. Crop Genetic Resources 4:2–7 (in Chinese)Google Scholar
  2. Anderson JA (2007) Marker-assisted selection for Fusarium head blight resistance in wheat. Int J Food Microbiol 119:51–53PubMedCrossRefGoogle Scholar
  3. Bai GH, Shaner G (2004) Management and resistance in wheat and barley to Fusarium head blight. Annu Rev Phytopathol 42:135–161PubMedCrossRefGoogle Scholar
  4. Bai GH, Kolb FL, Shaner G, Domier LL (1999) Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopathology 89:343–348PubMedCrossRefGoogle Scholar
  5. Bourdoncle W, Ohm HW (2003) Quantitative trait loci for resistance to Fusarium head blight in recombinant inbred wheat lines from the cross Huapei 57-2/Patterson. Euphytica 131:131–136CrossRefGoogle Scholar
  6. Buerstmayr H, Steiner B, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schneider B, Lemmens M (2003) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread. Theor Appl Genet 107:503–508PubMedCrossRefGoogle Scholar
  7. Buerstmayr H, Adam G, Lemmens M (2012) Resistance to head blight caused by Fusarium spp. in wheat. In: Sharma I (ed) Disease resistance in wheat. CABI, Wallingford, pp 236–276CrossRefGoogle Scholar
  8. CIMMYT (2005) Laboratory protocols: CIMMYT applied molecular genetics laboratory, 3rd edn. CIMMYT, MexicoGoogle Scholar
  9. Cuthbert PA, Somers DJ, Brulé-Babel A (2007) Mapping of Fhb2 on chromosome 6BS: a gene controlling Fusarium head blight field resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet 114:429–437PubMedCrossRefGoogle Scholar
  10. Duveiller E, Mezzalama M, Murakami J, Lewis J, Ban T (2008) Global fusarium networking. Cereal Res Commun 36:11–19CrossRefGoogle Scholar
  11. Emrich K, Wilde F, Miedaner T, Piepho HP (2008) REML approach for adjusting the Fusarium head blight rating to a phenological date in inoculated selection experiments of wheat. Theor Appl Genet 117:65–73PubMedCrossRefGoogle Scholar
  12. Gilchrist L, Rajaram S, Mujeeb-Kazi A, van Ginkel M, Vivar H, Pfeiffer W (1996) Fusarium scab screening program at CIMMYT. In: Dubin HJ, Gilchrist L, Reeves J, McNab A (eds) Fusarium head scab: global status and future prospects. CIMMYT, Mexico D.F., pp 7–12Google Scholar
  13. Graham S, Browne RA (2009) Anther extrusion and Fusarium head blight resistance in European wheat. J Phytopathol 157:580–582CrossRefGoogle Scholar
  14. He ZH, van Ginkel M, Gilchrist L, Rajaram S (2000) Progress of China/CIMMYT shuttle breeding and germplasm exchange aimed at combining high yield potential with scab resistance. Proc Natl Fusarium Head Blight Forum, Cincinnati, Ohio, USA, pp 264–268Google Scholar
  15. He ZH, Rajaram S, Xin ZY, Huang GZ (eds) (2001) A history of wheat breeding in China. CIMMYT, MexicoGoogle Scholar
  16. He X, Singh PK, Duveiller E, Schlang N, Dreisigacker S, Singh RP (2013) Identification and characterization of international Fusarium head blight screening nurseries of wheat at CIMMYT, Mexico. Eur J Plant Pathol 136:123–134CrossRefGoogle Scholar
  17. Jiang GL, Shi JR, Ward RW (2007) QTL analysis of resistance to Fusarium head blight in the novel wheat germplasm CJ 9306. I. Resistance to fungal spread. Theor Appl Genet 116:3–13PubMedCrossRefGoogle Scholar
  18. Kumar S, Stack RW, Friesen TL, Faris JD (2007) Identification of a novel Fusarium head blight resistance quantitative trait locus on chromosome 7A in tetraploid wheat. Phytopathology 97:592–597PubMedCrossRefGoogle Scholar
  19. Li T, Bai G, Wu S, Gu S (2011) Quantitative trait loci for resistance to Fusarium head blight in a Chinese wheat landrace Haiyanzhong. Theor Appl Genet 122:1497–1502PubMedCrossRefGoogle Scholar
  20. Li T, Bai GH, Wu SY, Gu SL (2012) Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu. Euphytica 185:93–102CrossRefGoogle Scholar
  21. Lin F, Kong ZX, Zhu HL, Xue SL, Wu JZ, Tian DG, Wei JB, Zhang CQ, Ma ZQ (2004) Mapping QTL associated with resistance to Fusarium head blight in the Nanda 2419 x Wangshuibai population. I. Type II resistance. Theor Appl Genet 109:1504–1511CrossRefGoogle Scholar
  22. Liu ZZ, Wang ZY, Huang DC, Zhao WJ, Huang XM (1997) Improvement of CIMMYT wheat germplasm for scab resistance and its utilization in Shanghai. In: He Z, Rajaram S (eds) China/CIMMYT collaboration on wheat breeding and germplasm exchange: Results of 10 years of shuttle breeding (1984–94). Wheat Special Report No 46. CIMMYT, Mexico, pp 28–32Google Scholar
  23. Liu S, Chalhoub B, Dolezel J, Zhang JX, Trick HN, Gill BS, Pumphrey MO, Anderson JA (2008) Toward positional cloning of Fhb1, a major QTL for Fusarium head blight resistance in wheat. Cereal Res Commun 36:195–201CrossRefGoogle Scholar
  24. Liu SY, Hall MD, Griffey CA, McKendry AL (2009) Meta-analysis of QTL associated with Fusarium head blight resistance in wheat. Crop Sci 49:1955–1968CrossRefGoogle Scholar
  25. Liu Y, He Z, Appels R, Xia X (2012) Functional markers in wheat: current status and future prospects. Theor Appl Genet 125:1–10PubMedCrossRefGoogle Scholar
  26. Lu W, Cheng S, Liu Y (2001) Wheat scab research in China. Science Publishing Company, Beijing (in Chinese)Google Scholar
  27. Lu Q, Lillemo M, Skinnes H, He X, Shi J, Ji F, Dong Y, Bjornstad A (2013) Anther extrusion and plant height are associated with Type I resistance to Fusarium head blight in bread wheat line ‘Shanghai-3/Catbird’. Theor Appl Genet 126:317–334PubMedCrossRefGoogle Scholar
  28. Mesterhazy A, Bartok T, Mirocha CG, Komoroczy R (1999) Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breed 118:97–110CrossRefGoogle Scholar
  29. Mesterhazy A, Bartok T, Lamper C (2003) Influence of wheat cultivar, species of Fusarium, and isolate aggressiveness on the efficacy of fungicides for control of Fusarium head blight. Plant Dis 87:1107–1115CrossRefGoogle Scholar
  30. Ortiz R, Braun H-J, Crossa J, Crouch JH, Davenport G, Dixon J, Dreisigacker S, Duveiller E, He Z, Huerta J, Joshi AK, Kishii M, Kosina P, Manes Y, Mezzalama M, Morgounov A, Murakami J, Nicol J, Ortiz Ferrara G, Ortiz-Monasterio JI, Payne TS, Peña RJ, Reynolds MP, Sayre KD, Sharma RC, Singh RP, Wang J, Warburton M, Wu H, Iwanaga M (2008) Wheat genetic resources enhancement by the International Maize and Wheat Improvement Center (CIMMYT). Genet Resour Crop Evol 55:1095–1140CrossRefGoogle Scholar
  31. Otto CD, Kianian SF, Elias EM, Stack RW, Joppa LR (2002) Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat. Plant Mol Biol 48:625–632PubMedCrossRefGoogle Scholar
  32. Paul PA, Lipps PE, Madden LV (2005) Relationship between visual estimates of Fusarium head blight intensity and deoxynivalenol accumulation in harvested wheat grain: a meta-analysis. Phytopathology 95:1225–1236PubMedCrossRefGoogle Scholar
  33. Ruckenbauer P, Buerstmayr H, Lemmens M (2001) Present strategies in resistance breeding against scab (Fusarium spp.). Euphytica 119:121–127CrossRefGoogle Scholar
  34. Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838Google Scholar
  35. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRefGoogle Scholar
  36. Shen X, Zhou M, Lu W, Ohm H (2003) Detection of Fusarium head blight resistance QTL in a wheat population using bulked segregant analysis. Theor Appl Genet 106:1041–1047PubMedGoogle Scholar
  37. Skinnes H, Semagn K, Tarkegne Y, Marøy AG, Bjørnstad Å (2010) The inheritance of anther extrusion in hexaploid wheat and its relationship to Fusarium head blight resistance and deoxynivalenol content. Plant Breed 129:149–155CrossRefGoogle Scholar
  38. Somers DJ, Fedak G, Savard M (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome 46:555–564PubMedCrossRefGoogle Scholar
  39. Steiner B, Lemmens M, Griesser M, Scholz U, Schondelmaier J, Buerstmayr H (2004) Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theor Appl Genet 109:215–224PubMedCrossRefGoogle Scholar
  40. Wan YF, Yen C, Yang JL (1997) Sources of resistance to head scab in Triticum. Euphytica 94:31–36CrossRefGoogle Scholar
  41. Wegulo SN, Bockus WW, Nopsa JH, De Wolf ED, Eskridge KM, Peiris KHS, Dowell FE (2011) Effects of integrating cultivar resistance and fungicide application on Fusarium head blight and deoxynivalenol in winter wheat. Plant Dis 95:554–560CrossRefGoogle Scholar
  42. Xue S, Li GQ, Jia HY, Xu F, Lin F, Tang MZ, Wang Y, An X, Xu HB, Zhang LX, Kong ZX, Ma ZQ (2010) Fine mapping Fhb4, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet 121:147–156PubMedCrossRefGoogle Scholar
  43. Xue S, Xu F, Tang M, Zhou Y, Li G, An X, Lin F, Xu H, Jia H, Zhang L, Kong Z, Ma Z (2011) Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet 123:1055–1063PubMedCrossRefGoogle Scholar
  44. Yang ZP, Gilbert J, Procunier JD (2006) Genetic diversity of resistance genes controlling Fusarium head blight with simple sequence repeat markers in thirty-six wheat accessions from east asian origin. Euphytica 148:345–352CrossRefGoogle Scholar
  45. Yu J-B, Bai G-H, Cai S-B, Dong Y-H, Ban T (2008) New Fusarium head blight-resistant sources from Asian wheat germplasm. Crop Sci 48:1090–1097CrossRefGoogle Scholar
  46. Zhang X, Pan H, Bai G (2012) Quantitative trait loci responsible for Fusarium head blight resistance in Chinese landrace Baishanyuehuang. Theor Appl Genet 125:495–502PubMedCrossRefGoogle Scholar
  47. Zhou C (2003) Breeding for Fusarium head blight in wheat. In: Zhuang Q (ed) Chinese wheat improvement and pedigree analysis. China Agriculture Press, Beijing, pp 444–469 (in Chinese) Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Xinyao He
    • 1
    • 2
  • Pawan K. Singh
    • 1
  • Norbert Schlang
    • 1
  • Etienne Duveiller
    • 1
  • Susanne Dreisigacker
    • 1
  • Thomas Payne
    • 1
  • Zhonghu He
    • 3
  1. 1.Global Wheat ProgramInternational Maize and Wheat Improvement Center (CIMMYT)MexicoMexico
  2. 2.Department of Plant and Environmental SciencesNorwegian University of Life SciencesÅsNorway
  3. 3.International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAASBeijingChina

Personalised recommendations