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Euphytica

, Volume 191, Issue 1, pp 85–98 | Cite as

VvGAI1 polymorphisms associate with variation for berry traits in grapevine

  • Alba M. VargasEmail author
  • Loïc Le Cunff
  • Patrice This
  • Javier Ibáñez
  • M. Teresa de Andrés
Article

Abstract

Gibberellins have important effects over different developmental processes in plants and the exogenous application of GA3 has been extensively used in grapevine to improve certain quality traits (to control berry size, set and weight, to decrease cluster compactness and to induce the absence of seeds in grapes). In this study, we performed association analyses using a core collection of 127 table grape accessions in order to identify causal SNPs in the gene VvGAI1, a negative regulator of gibberellins response. Seventeen quantitative descriptors, related with fertility, bunch and berry traits were used for the association analysis with VvGAI1. Fifteen polymorphisms and 15 haplotypes were identified, and some of them associated significantly with important traits for table grape: berry texture, juice yield, and bunch weight. The genetic structure found in the collection of varieties was consistent with their origins, and was included in the association test. In addition, nucleotide and haplotypic diversity, linkage disequilibrium and the possible existence of selection over the gene were also evaluated to support the association test.

Keywords

Association analysis Table grape Vitis vinifera VvGAI1 

Notes

Acknowledgments

This study was made possible with the funding from the GrapeGen Project (joint venture between Genome Canada and Genoma España), and the AGL2010-15694 from the MICINN (Spain). A.M. Vargas was funded by a predoctoral fellowship from IMIDRA. We thank M. Carmen Fajardo, Carlos González Guillén, Nuria Rodríguez Jiménez, Concepción López Rivas, M. Dolores Vélez, Silvia Hernáiz and Paz Fernández for their technical assistance in the morphological descriptions.

Supplementary material

10681_2013_866_MOESM1_ESM.docx (52 kb)
Supplementary material 1 (DOCX 52 kb)
10681_2013_866_MOESM2_ESM.tif (110 kb)
Supplementary material 2 (TIFF 109 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Alba M. Vargas
    • 1
    Email author
  • Loïc Le Cunff
    • 2
    • 3
  • Patrice This
    • 2
  • Javier Ibáñez
    • 1
    • 4
  • M. Teresa de Andrés
    • 1
  1. 1.Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario (IMIDRA), Finca El EncínAlcalá de HenaresSpain
  2. 2.INRA UMR 1097 Diversité et Adaptation des Plantes CultivéesMontpellierFrance
  3. 3.UMT Geno-Vigne® (IFV, Supagro, INRA)MontpellierFrance
  4. 4.CSIC-Universidad de La Rioja-Gobierno de La RiojaLogroñoSpain

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