Euphytica

, Volume 190, Issue 3, pp 439–445 | Cite as

Reaction of sugar beet S1 lines and cultivars to different isolates of Macrophomina phaseolina and Rhizoctonia solani AG-2-2IIIB

Article

Abstract

Interactions of 17 sugar beet lines and cultivars with four isolates of Macrophomina phaseolina (the causal agent of charcoal rot) and one isolate of Rhizoctonia solani (the causal agent of crown and root rot) were studied in separate experiments under greenhouse conditions. The isolates of Macrophomina were taken from their host plants, sugar beet (two isolates), soybean and sesame. In the first experiment, the colonized toothpick was used as inoculum. In the second experiment, six-month-old sugar beet plants were inoculated with barley seeds colonized with M. phaseolina. For the inoculation of sugar beet lines with R. solani, the colonized corn seeds were used. Root symptoms were recorded four weeks after inoculation, by estimating the proportion of the root surface infected by the pathogens, using a 1–9 standard scale. Our results showed a significant difference among lines and cultivars in their resistance to these two pathogens. Line B8618 was found to be considerably resistant to the isolates of the both pathogens. The inoculation methods of Macrophomina isolates had no significant effect on the results. The interaction between isolate and cultivar was not also significant in Macrophomina-resistant lines. Therefore, it appears that the response of sugar beet lines to the tested fungal isolates was not differential. These resistant lines showed a high resistance to all the tested M. phaseolina isolates. Our results revealed that the Macrophomina-resistant lines also showed resistance to R. solani. Furthermore, the sugar beet drought tolerant lines (M293, M362 and M345) were susceptible to the tested M. phaseolina and R. solani isolates.

Keywords

Charcoal rot Drought Rhizoctoniasolani Sugar beet 

References

  1. Ahmad Y, Hameed A, Ghaffar A (2006) Enzymatic activity of fungal pathogens in corn. Pak J Bot 38(4):1305–1316Google Scholar
  2. Ahmadi M, Majidi-Heravan E, Sadeghian SY, Mesbah M, Darvish F (2011) Drought tolerance variability in S1 pollinator lines developed from a sugar beet open population. Euphytica 178:339–349CrossRefGoogle Scholar
  3. Alaghebandzadeh N, Rezaiee S, Mahmoudi B, Zamanizadeh H (2008) Pathogenic and genotypic analysis among Iranian isolates of Macrophomina phaseolina. Phytopathol 98:S11Google Scholar
  4. Almeida AMR, Abdelnoor RV, Arias CAA, Carvalho VP, Jacoud Filho DS, Marin SRR, Benato LC, Pinto MC, Carvalho CGP (2003) Genotypic diversity among brazilian isolates of Macrophomina phaseolina revealed by RAPD. Fitopatologia Brasileira 28:279–285CrossRefGoogle Scholar
  5. Amadioha AC (1998) Cellulolytic enzyme production by Rhizoctonia bataticola. Arch Phytopath Pflanz 31:415–421CrossRefGoogle Scholar
  6. Ashby SF (1927) Macrophomina phaseolina (Maubl.) Comb. Nov. The pycnidial stage of Rhizoctonia bataticola (Taub.) Butl. Trans Br Mycol Soc 12:141–147CrossRefGoogle Scholar
  7. Asher MJC, Hanson L (2006) Fungal and bacterial diseases. In: AP Draycott (Ed.) Sugar beet Blackwell publishing, pp 286–316Google Scholar
  8. Banihashemi Z (1998) Phytophthora rot of sugar beet root and sunflower stem in province of Fars Iran. Iran J Plant Pathol 4:239Google Scholar
  9. Beas-Fernandez R, De Santiago-De Santiago A, Hernandez-Delgado S, Mayek-Perez N (2006) Characterization of Mexican and non-Mexican isolates of Macrophomina phaseolina based on morphological characteristics, pathogenicity on bean seeds and endoglucanase genes. J Plant Pathol 88(1):53–60Google Scholar
  10. Bugbee WM (1993) A pectin lyase inhibitor protein from cell walls of sugar beet. Phytopathology 83:63–68CrossRefGoogle Scholar
  11. Buttner G, Pfahler B, Marlander B (2004) Greenhouse and field techniques for testing sugar beet for resistance to Rhizoctonia root and crown rot. Plant Breed 123:158–166CrossRefGoogle Scholar
  12. Cook DA, Scott RK (1993) The sugar beet crop: science into practice. Champan and Hall, New YorkCrossRefGoogle Scholar
  13. Ershad D (2009) Fungi of Iran. Iranian research institute of plant protection, Tehran 531 pp.Google Scholar
  14. Gaskill JO, Mumford DL, Ruppel EG (1970) Preliminary report on breeding for combined resistance to leaf spot, curly top and Rhizoctonia. J Am Soc Sugar Beet Tech 16:207–213CrossRefGoogle Scholar
  15. Hecker RJ, Ruppel EG (1977) Rhizoctonia root rot resistance in sugar beet: breeding and related research. J Am Soc Sugar Beet Technol 19:246–256Google Scholar
  16. Holliday P, Punithalingam E (1970) Macrophomina phaseolina. Descriptions of pathogenic fungi and Bacteria No. 275, Commonweslth Mycological Institiue, EnglandGoogle Scholar
  17. Jones RW, Canada S, Wang H (1998) Highly variable minichoromosomes and highly conserved endoglucanase genes in the phytopathogenic fungus Macrophomina phaseolina. Can J Bot 76:694–698Google Scholar
  18. Mahmoudi, SB, Soltani J (2005) Sugar beet root rot in Iran. Newsletter of Iranian Sugar Industries Research and Training Center, 16(178): 14–18 Google Scholar
  19. Mahmoudi SB, Mesbah M, Alizadeh A (2004) Pathogenic variability of Rhizoctonia solani in sugar beet. Iran J Plant pathol 40:253–280Google Scholar
  20. Mahmoudi SB, Mesbah M, Rahimian H, Noruzi P (2005) Genetic diversity of sugar beet isolates of Rhizoctonia solani revealed by RAPD-PCR and ITS-rDNA analysis. Iran J Plant pathol 41:523–542Google Scholar
  21. Marcus L, Barash I, Sneh B, Koltin Y, Finkler A (1986) Purification and characterization of pectinolytic enzymes produced by virulent and hypovirulent isolates of Rhizoctonia solani. Physiol Mol Plant Pathol 29:325–336CrossRefGoogle Scholar
  22. Martin FN, English JT (1997) Population genetics of soilborne fungal plant pathogens. Phytopathol 87:446–447CrossRefGoogle Scholar
  23. Mayek-Perez N, Garcia–Espinosa R, Lopez-Castaneda C, Acosta-Gallegos JA, Simpson J (2002) water relation, histopathology and growth of common bean (Phaseolus vulgaris L.) during pathogenesis of Macrophomina phaseolina under drought stress. Physiol Mol Plant Pathol 60:185–195Google Scholar
  24. Mayek-Perez N, Lopez-Castaneda C, Gonzalez-Chavira M, Garcia–Espinosa R, Acosta-Gallegos JA, De la Martinez-Vega O, Simpson J (2001) Variability of Mexican isolates of Macrophomina phaseolina on basis of pathogenesis and AFLP genotype. Physiol Mol Plant Pathol 59:257–264CrossRefGoogle Scholar
  25. McDonald BA, Linde C (2002) Pathogen population genetics, evolutionary potential, and durable resistance. Annu Rev Phytopathol 40:349–379PubMedCrossRefGoogle Scholar
  26. Naito S, Sugitomo T (1981) Histopathological observation on root rot of sugar beet by different anastomosis groups of Rhizoctonia solani. Hokkaido Natl Agr Exp Sta Res Bull 131:95–110Google Scholar
  27. Pearson CAS, Leslie JF, Schwenk FW (1987) Host preference correlated with chlorate resistance in Marophomina phaseolina. Plant Dis 71:828–831CrossRefGoogle Scholar
  28. Raeyatpanah S, Alavi SV, Arab G (2007) Reaction of some soybean advanced lines to charcoal rot disease, Macrophomina phaseolina (Tassi) Goid in East Mazandaran. Seed Plant J 23:181–189Google Scholar
  29. Raoufi M, Farrokhonejad R, Mahmoudi SB (2003) Identification and pathogenicity of Fusarium species associated with sugar beet root and crown rot in Iran. Sugar Beet J 19(2):109–122Google Scholar
  30. Ruppel EG (1972) Correlation of cultural characters and source of isolates with pathogenicity of Rhizoctonia solani from sugar beet. Phytopathol 62:202–205CrossRefGoogle Scholar
  31. Scholten OE, Panella LW, DeBock TSM, Lange W (2001). A greenhouse test for screening sugarbeet (Beta vulgaris) for resistance to Rhizoctonia solani. Eur J Plant Pathol 107: 161–166Google Scholar
  32. Schuster ML, Jensen SG, Sayre RM (1958) Toothpick method of inoculating sugar beets for determining pathogenicity of Rhizoctonia solani. J Am Soc Sugar Beet Technol 10:142–149CrossRefGoogle Scholar
  33. Sheikholeslami M, Hajaroud G, Okhovat M (1998) Fungi causing sugar beet post-harvest root rot in Kermanshah. Iran J Plant Pathol 34:84–92Google Scholar
  34. Tomkins CM (1938) Charcoal rots of sugar beet. Hilgardia 12(1):75–81Google Scholar
  35. van den Boogert PHJF, Bonants PJM, Schneider JHM (1998) Molecular detection of pathogenic subgroups of Rhizoctonia silani AG-2-2. 7th Int Cong Plant Pathol: 3.3.74Google Scholar
  36. Vandermark G, Martinez O, Pecina V, Alvarado MJ (2000) Assessment of genetic relationships among isolates of Macrophomina phaseolina using a simplified AFLP technique and two different methods of analysis. Mycologia 92:659–664Google Scholar
  37. Whitney ED, Duffus JE (1986) Compendium of beet diseases and insects. APS pressGoogle Scholar
  38. Windels CE, Panella LW, Ruppel EG (1995) Sugar beet germplasm resistant to Rhizoctonia root and crown rot withstands disease caused by several pathogenic isolates of Rhizoctonia solani AG-2-2. Sugar beet. Research and Extension Reports, 26: 179–185Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Sugar Beet Seed InstituteKarajIslamic Republic of Iran
  2. 2.Islamic Azad University, Science and Research BranchTehranIran

Personalised recommendations