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Identification of major QTLs associated with stable resistance of tomato cultivar ‘Hawaii 7996’ to Ralstonia solanacearum

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Bacterial wilt caused by Ralstonia solanacearum is one of the most devastating diseases of tomato. Tomato cultivar ‘Hawaii 7996’ has been shown to have stable resistance against different strains under different environments. This study aimed to locate quantitative trail loci (QTLs) associated with stable resistance using 188 recombinant inbred lines (RILs) derived from ‘Hawaii 7996’ and ‘West Virginia 700.’ A new linkage map with good genome coverage was developed, mainly using simple sequence repeat markers developed from anchored bacterial artificial chromosome or scaffold sequences of tomato. The population was evaluated against phylotype I and phylotype II strains at seedling stage or in the field in Indonesia, Philippines, Taiwan, Thailand, and Reunion. Two major QTLs were identified to be associated with stable resistance. Bwr-12, located in a 2.8-cM interval of chromosome 12, controlled 17.9–56.1 % of total resistance variation. The main function of Bwr-12 was related to suppression of internal multiplication of the pathogen in the stem. This QTL was not associated with resistance against race 3-phylotype II strain. Bwr-6 on chromosome 6 explained 11.5–22.2 % of the phenotypic variation. Its location differed with phenotype datasets and was distributed along a 15.5-cM region. The RILs with the resistance allele from both Bwr-12 and Bwr-6 had the lowest disease incidence, which was significantly lower than the groups with only Bwr-12 or Bwr-6. Our studies confirmed the polygenic nature of resistance to bacterial wilt in tomato, and that stable resistance in ‘Hawaii 7996’ is mainly associated with Bwr-6 and Bwr-12.

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  1. Ashrafi H, Kinkade M, Foolad MR (2009) A new genetic linkage map of tomato based on a Solanum lycopersicum × S. pimpinellifolium RIL population displaying locations of candidate pathogen response genes. Genome 52:935–956

  2. Balatero C (2000) Genetic analysis and molecular mapping of bacterial wilt resistance in tomato (Lycopersicon esculentum Mill.). Ph.D. Dissertation, University of the Philippines Los Baños, Philippines, p 147

  3. Balatero CH, Hautea DM, Narciso JO, Hanson PM (2005) QTL mapping for bacterial wilt resistance in Hawaii 7996 using AFLP, RGA, and SSR markers. In: Allen C, Prior P, Hayward AC (eds) Bacterial wilt disease and the Ralstonia solanacearum species complex. APS Press, St. Paul, pp 301–308

  4. Burr B, Burr FA (1991) Recombinant inbreds for molecular mapping in maize: theoretical and practical considerations. Trends Genet 7:55–60

  5. Carmeille A, Prior P, Kodja H, Chiroleu F, Luisetti J, Besse P (2006a) Evaluation of resistance to race 3, biovar 2 of Ralstonia solanacearum in tomato germplasm. J Phytopathol 154:398–402

  6. Carmeille A, Caranta C, Dintinger J, Prior P, Luisetti J, Besse P (2006b) Identification of QTLs for Ralstonia solanacearum race 3-phylotype II resistance in tomato. Theor Appl Genet 113:110–121

  7. Cellier G, Prior P (2010) Deciphering phenotypic diversity of Ralstonia solanacearum strains pathogenic to potato. Phytopathology 100:1250–1261

  8. Danesh D, Aarons S, Mcgill GE, Young ND (1994) Genetic dissection of oligogenic resistance to bacterial wilt in tomato. Mol Plant Microbe Interact 7:464–471

  9. Deberdt P, Oliver J, Thoquet P, Quénéhervé P, Prior P (1999) Evaluation of bacterial wilt resistance in tomato lines nearly isogenic for the Mi gene for resistance to root-knot. Plant Pathol 48:415–424

  10. Denny TP (2006) Plant pathogenic Ralstonia species. In: Gnanamanickam SS (ed) Plant-associated bacteria. Springer, Dordrecht, pp 573–644

  11. Deslandes L, Pileur F, Liaubet L et al (1998) Genetic characterization of RRS1, a recessive locus in Arabidopsis thaliana that confers resistance to the bacterial soilborne pathogen Ralstonia solanacearum. Mol Plant Microbe Interact 11:659–667

  12. Deslandes L, Olivier J, Peeters N, Feng, DX, Khounlotham M, Boucher C, Somssich I, Genin S, Marco Y (2003) Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus. Proc Natl Acad Sci USA 100(13):8024–8029

  13. Dickinson M, Jones DA, Jones JDG (1993) Close linkage between the Cf-2/Cf-5 and Mi resistance loci in tomato. Mol Plant Microbe Interact 6:341–347

  14. Elphinstone JG (2005) The current bacterial wilt situation: a global overview. In: Allen C, Prior P, Hayward AC (eds) Bacterial wilt disease and the Ralstonia solanacearum species complex. APS Press, St. Paul, pp 9–28

  15. Fegan M, Prior P (2005) How complex is the “Ralstonia solanacearum species complex”? In: Allen C, Prior P, Hayward AC (eds) Bacterial wilt disease and the Ralstonia solanacearum species complex. APS Press, St. Paul, pp 449–461

  16. Geethanjali S, Chen KY, Pastrana DV, Wang JF (2010) Development and characterization of tomato SSR markers from genomic sequences of anchored BAC clones on chromosome 6. Euphytica 173:85–91

  17. Geethanjali S, Kadirvel P, de la Peña R, Rao ES, Wang JF (2011) Development of tomato SSR markers from anchored BAC clones of chromosome 12 and their application for genetic diversity analysis and linkage mapping. Euphytica 178:283–295

  18. Grimault V, Prior P (1993) Bacterial wilt resistance in tomato associated with tolerance of vascular tissues to Pseudomonas solanacearum. Plant Pathol 42:589–594

  19. Grimault V, Anais G, Prior P (1994) Distribution of Pseudomonas solanacearum in the stem tissues of tomato plants with different levels of resistance to bacterial wilt. Plant Pathol 43:663–668

  20. Hai TTH, Esch E, Wang JF (2008) Resistance to Taiwanese race 1 strains of Ralstonia solanacearum in wild tomato germplasm. Eur J Plant Pathol 122:471–479

  21. Hanson PM, Wang JF, Licardo O, Hanudin, Mah SY, Hartman GL, Lin YC, Chen JT (1996) Variable reaction of tomato lines to bacterial wilt evaluated at several locations in Southeast Asia. HortScience 31:143–146

  22. Hayward AC (1991) Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu Rev Phytopathol 29:65–87

  23. Jaunet T, Wang JF (1999) Variation in genotype and aggressiveness diversity of Ralstonia solanacearum race 1 isolated from tomato in Taiwan. Phytopathology 89:320–327

  24. Joehanes R, Nelson JC (2008) QGene 4.0, an extensible Java QTL-analysis platform. Bioinformatics 24:2788–2789

  25. Kado CI, Heskett MG (1970) Selective media for isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas, and Xanthomonas. Phytopathology 60:969–976

  26. Kelman A (1954) The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology 44:693–695

  27. Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

  28. Lebeau A, Daunay MC, Frary A, Palloix A, Wang JF, Dintinger J, Chiroleu F, Wicker E, Prior P (2011) Bacterial wilt resistance in tomato, pepper, and eggplant: genetic resources respond to diverse strains in the Ralstonia solanacearum species complex. Phytopathology 101:154–165

  29. Lin CH, Hsu ST, Tzeng KC, Wang JF (2009) Detection of race 1 strains of Ralstonia solanacearum in field samples in Taiwan using a BIO-PCR method. Eur J Plant Pathol 124:75–85

  30. Lopes CA, Quezado Soares AM, De Melo PE (1994) Differential resistance of tomato cultigens to biovars I and III of Pseudomonas solanacearum. Plant Dis 78:1091–1094

  31. Mangin B, Thoquet P, Olivier J, Grimsley NH (1999) Temporal and multiple quantitative trait loci analyses of resistance to bacterial wilt in tomato permit the resolution of linked loci. Genetics 151:1165–1172

  32. Prior P, Steva H, Cadet P (1990) Aggressiveness of strains of Pseudomonas solanacearum from the French West Indies (Martinique and Guadeloupe) on tomato. Plant Dis 74:962–965

  33. Scott JW, Wang JF, Hanson PM (2005) Breeding tomatoes for resistance to bacterial wilt, a Global view. Acta Hortic 695:161–172

  34. Seifi A, Kaloshian I, Vossen J et al (2011) Linked, if not the same, Mi-1 homologues confer resistance to tomato powdery mildew and root-knot nematodes. Mol Plant Microbe Interact 24:441–450

  35. Thoquet P, Olivier J, Sperisen C, Rogowsky P, Laterrot H, Grimsley NH (1996a) Quantitative trait loci determining resistance to bacterial wilt in tomato cultivar Hawaii 7996. Mol Plant Microbe Interact 9:826–836

  36. Thoquet P, Olivier J, Sperison C et al (1996b) Polygenic resistance of tomato plants to bacterial wilt in the French West Indies. Mol Plant Microbe Interact 9:837–842

  37. Tomato Genome Consortium (2012) The tomato genome sequence provide insights into fleshy evolution. Nature 485:635–641

  38. Truong HTH, Graham E, Esch E, Wang JF, Hanson P (2010) Distribution of DArT markers in a genetic linkage map of tomato. Korean J Hortic Sci Technol 28:664–671

  39. Tsai JW, Hsu ST, Chen LC (1985) Bacteriocin-producing strains of Pseudomonas solanacearum and their effect on development of bacterial wilt of tomato. Plant Prot Bull 27:267–278

  40. Vailleau F, Sartorel E et al (2007) Characterization of the interaction between the bacterial wilt pathogen Ralstonia solanacearum and the model legume plant Medicago truncatula. Mol Plant Microbe Interact 20:159–167

  41. Van Ooijen JW (2006) JoinMap Version 4.0, software for the calculation of genetic linkage maps. Kyazma B.V., Wageningen

  42. Vasse J, Frey P, Trigalet A (1995) Microscopic studies of intercellular infection and protoxylem invasion of tomato roots by Pseudomonas solanacearum. Mol Plant Microbe Interact 8:241–251

  43. Verlaan MG, Szinay D, Hutton SF, de Jong H, Kormelink R, Visser RG, Scott JW, Bai Y (2011) Chromosomal rearrangements between tomato and Solanum chilense hamper mapping and breeding of the TYLCV resistance gene Ty-1. Plant J 68:1093–1103

  44. Wang JF, Hanson PM, Barnes JA (1998). Worldwide evaluation of an international set of resistance sources to bacterial wilt in tomato. In: Prior P, Allen C, and Elphinstone J (eds). Bacterial wilt disease: molecular and ecological aspects. Springer, Berlin, pp 269–275

  45. Wang JF, Olivier J, Thoquet P, Mangin B, Sauviac L, Grimsley NH (2000) Resistance of tomato line Hawaii 7996 to Ralstonia solanacearum Pss4 in Taiwan is controlled mainly by a major strain-specific locus. Mol Plant Microbe Interact 13:6–13

  46. Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

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The authors would like to thank Ms. Dolores R. Ledesma for conducting statistical analyses and Ms. Chiou-fen Hsu for technical assistance. This study is supported by funding provided by GTZ 81070160: Deutsche Gesellschaft für Internationale Zusammenarbeit GmbH of Germany and COA 98as-1.2.1-s-ag: Council of Agriculture of Taiwan.

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Correspondence to Jaw-Fen Wang.

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Wang, J., Ho, F., Truong, H.T.H. et al. Identification of major QTLs associated with stable resistance of tomato cultivar ‘Hawaii 7996’ to Ralstonia solanacearum . Euphytica 190, 241–252 (2013). https://doi.org/10.1007/s10681-012-0830-x

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  • Bacterial wilt
  • Simple sequence repeat
  • Linkage map
  • Solanum lycopersicum